Serological, Enterotoxin-Producing and Biochemical Properties of Escherichia Coli Isolated from Piglets with Neonatal Diarrhea in Norway

Lund, Arve; Fossum, Kåre; Liven, Eivind

doi:10.1186/bf03546824

Cited by 11 publications

(4 citation statements)

References 11 publications

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“…This correlation is noteworthy, as the production of LT enterotoxin is rarely observed in strains lacking the F4 antigen or producing the F6 antigen (8,19,24,31). However, F4-negative, LT-positive 08 strains have been reported on several occasions (8,17,18). Our results suggest that LT enterotoxin may be an important virulence factor for the E. coli 08:KX105 strains.…”

Section: Discussionmentioning

confidence: 49%

Virulence properties of enterotoxigenic Escherichia coli O8: KX105 strains isolated from diarrheic piglets

et al. 1988

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Twenty-two enterotoxigenic Escherichia coli (ETEC) O8:KX105 strains isolated from 1- to 7-week-old diarrheic piglets were examined for virulence properties. Thirteen strains caused acute watery diarrhea in orally infected, colostrum-deprived newborn piglets, whereas the remaining nine did not. The enteropathogenic strains colonized the small intestine, albeit with lower intensity than classical porcine ETEC. They produced the heat-stable STb and heat-labile LT enterotoxins, whereas the nonenteropathogenic strains produced the STb enterotoxin alone. None of the E. coli O8:KX105 strains exhibited mannose-resistant hemagglutination with erythrocytes from 12 species. Ten of the enteropathogenic and two of the nonenteropathogenic strains were positive for mannose-sensitive hemagglutination. These strains produced rodlike fimbriae 3 to 5 nm in diameter, whereas no fimbriae were detected on the other strains. None of the 22 strains produced the fimbrial antigens F4, F5, F41, F2, F3, FY(Att 25), and F165. Of the 13 enteropathogenic strains, 10 expressed the F6 antigen in the intestines of infected piglets but not in cultures. The other three enteropathogenic strains apparently lacked all of the known fimbrial antigens from porcine ETEC.

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Section: Discussionmentioning

confidence: 49%

Virulence properties of enterotoxigenic Escherichia coli O8: KX105 strains isolated from diarrheic piglets

et al. 1988

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“…It appears that E . coli strains belonging to 0 groups 149 and 64 associated with the adhesion fimbria antigens F4 and F5 respectively, are those mainly responsible for E. coli diarrhea in pigs in Norway at the present time (Lund et al 1982). For diagnostic purposes, it is important to determine the frequency in which enterotoxigenic E. coli strains are found in the intestines of pigs dying from diarrhea.…”

Section: Discussionmentioning

confidence: 99%

“…Enterotoxigenic strains of E. coli inducing diarrhea, usually possess the ability to adhere to the intestinal surface and to produce enterotoxins (Satterwith et al 1978, Back et al 1980, DeBoy et at, 1981, Lund et al 1982, Gaastra & Graaf 1982. The adhesive fimbria of E .…”

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confidence: 99%

Enterotoxigenic Escherichia Coli in The Jejunum of Piglets with Neonatal Diarrhea

Olsvik¹,

Fossum²

1984

Acta Vet Scand

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“…Final viable counts (the average of two duplicates) were obtained as described. 27 Experimental animals and experimental design. Healthy newborn, colostrum-deprived Large White piglets were obtained from sows held at the Pinjarra Hills Piggery Unit at The University of Queensland.…”

Section: In Vitro Adhesion Assaymentioning

confidence: 99%

Pathogenicity of Vietnamese Enterotoxigenic Escherichia coli Strains in Colostrum-deprived One-day-old Piglets

Wilkie

Driesen³

et al. 2006

Vet Pathol

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Abstract. Preweaning colibacillosis is a major cause of economic loss to the swine industry in Vietnam. The aim of this study was to examine the enteropathogenicity of representative enterotoxigenic Escherichia coli (ETEC) strains obtained during an earlier epidemiologic survey conducted in five provinces in North Vietnam. This included isolates belonging to serotype O8 that produced heat-stable and heat-labile enterotoxins but did not produce any of the recognized fimbriae (F4, F5, F6, F41, F18). In vitro hemagglutination (unique mannose-resistant hemagglutination activity with guinea pig, sheep, human, and chicken red blood cells at 37uC, but not at 18uC) and enterocyte brush border attachment assays suggested that the F-ETEC strains produced an unidentified colonization factor that promoted adherence to the intestinal epithelium. Colostrum-deprived 1-day-old piglets challenged with an Fstrain (1-2 3 10 9 bacteria) developed acute watery diarrhea within 4 hours of inoculation and suffered up to 20% weight loss, with comparable severity to piglets challenged with conventional F4 and F5 strains. At necropsy, viable counts and histopathologic examination of intestinal sections demonstrated colonization of the duodenum, jejunum, and ileum by F4-positive strains. In comparison, the F-and F5-positive strains attached exclusively to the ileum. Transmission electron micrographs of negatively stained F-cells grown at 37uC demonstrated the presence of fimbriae. These results confirm the presence of a potentially new pathogenic ETEC fimbrial type in piggeries in Vietnam, with a unique hemagglutination property and attachment characteristics similar to ETEC bearing F5 fimbriae.

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Serological, Enterotoxin-Producing and Biochemical Properties of Escherichia Coli Isolated from Piglets with Neonatal Diarrhea in Norway

Cited by 11 publications

References 11 publications

Virulence properties of enterotoxigenic Escherichia coli O8: KX105 strains isolated from diarrheic piglets

Virulence properties of enterotoxigenic Escherichia coli O8: KX105 strains isolated from diarrheic piglets

Enterotoxigenic Escherichia Coli in The Jejunum of Piglets with Neonatal Diarrhea

Pathogenicity of Vietnamese Enterotoxigenic Escherichia coli Strains in Colostrum-deprived One-day-old Piglets

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