Serological Diagnosis of Staphylococcal Bacteraemia

Christensson, Bértil; Herzog, Christian; Just, Vroni; Berger, Rosmarie; Just, M

doi:10.1016/s0140-6736(86)90579-9

Cited by 6 publications

(9 citation statements)

References 8 publications

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“…Since many antibodies against S. aureus also cross-react with simila/" structures in other, mainly Gram-positive, bacteria, the interpretation of a positive serological result should always be done with precaution if cultures are negative. However, it has been shown previously that once S. aureus bacteraemia is confirmed by positive blood cultures, various serological tests or test combinations can help to discriminate between patients with an uncomplicated from those with a complicated course of infection [13].…”

Section: Discussionmentioning

confidence: 99%

“…The clinical diagnosis of compli-cated septicaemia may be difficult to establish. The results from staphylococcal serology has previously been shown to add valuable information [6][7][8][9][12][13][14][15][16][17][18]. No single serological assay could be used to diagnose all patients with complicated septicaemia, with or without endocarditis, but the combined use of two or more tests was shown to increase both the specificity and the sensitivity of diagnosis [6,7,13,18].…”

Section: Introductionmentioning

confidence: 99%

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Antibody response to Staphylococcus aureus whole cell, lipase and staphylolysin in patients with S. aureus infections

Ryding¹

1992

FEMS Microbiology Letters

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Three assays to measure antibodies against Staphylococcus aureus whole cells, lipase and staphylolysin were used to try to discriminate between complicated and uncomplicated S. aureus septicaemia. Sera were examined from 8 patients with S. aureus endocarditis, 23 patients with complicated S. aureus septicaemia, 12 patients with uncomplicated S. aureus septicaemia and 93 febrile non‐septicaemic controls. No single assay could distinguish between complicated and uncomplicated S. aureus septicaemia. If the criterion for a positive result is defined as positive antibody level in the anti‐lipase ELISA as well as in at least 1 of the other 2 assays, 10/31 patients with S. aureus endocarditis or complicated septicaemia were positive compared to 0/93 non‐septicaemic patients and 0/12 patients with uncomplicated S. aureus septicaemia. Therefore, the combined use of serological assays in the diagnosis of complicated S. aureus septicaemia, one of which is the anti‐lipase ELISA, is recommended.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibody response to Staphylococcus aureus whole cell, lipase and staphylolysin in patients with S. aureus infections

Ryding¹

1992

FEMS Microbiology Letters

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“…The humoral antibody response [8][9][10]] to 5. aureus exotoxins, exoenzymes, cellular (cell wall) components, such as peptidoglycan (PG), teichoic acid (TA), and capsules [11], has not been resolved completely. Various investigators examined patient sera for anti bodies against certain S. aureus exotoxins, specifically a-hemolysin [12][13][14][15], enterotoxins A, B, and C [12], toxic shock syndrome toxin-1 [12], staphylolysin [16], and lipase [9,14,[16][17][18].…”

Section: Introductionmentioning

confidence: 99%

“…Various investigators examined patient sera for anti bodies against certain S. aureus exotoxins, specifically a-hemolysin [12][13][14][15], enterotoxins A, B, and C [12], toxic shock syndrome toxin-1 [12], staphylolysin [16], and lipase [9,14,[16][17][18]. Previously, various authors had tested various cell wall components of .S' .…”

Section: Introductionmentioning

confidence: 99%

“…Previously, various authors had tested various cell wall components of .S' . au reus for immunogenicity and sérodiagnostic utility, such as protein A [19], PG [9,[20][21][22][23], and teichoic acid or lipoteichoic acid [13,14,22,[24][25][26][27][28][29][30][31][32][33][34][35][36]. Perousal of the literature indi cated that the enzyme-linked immunosor bent assay (ELISA) largely had replaced oth er methods such as Ouchterlony double dif fusion in agar [26], countercurrent immunoelectrophoresis [29], and the radioimmu noassay [37], Most important, none of the tests for antibodies against exoenzymes, exo toxins, or cell wall components was capable of separating patients with complicated in fection (e.g., endocarditis, septicemia, osteo…”

Section: Introductionmentioning

confidence: 99%

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Immunobiology of Methicillin-Resistant <i>Staphylococcus aureus</i>: Immune Response of Rabbits and Patients to Systemic Infection

Traub

Bauer²,

Leonhard³

1996

Chemotherapy

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Teichoic acid (TA) and peptidoglycan (PG) extracted from Staphylococcus aureus strains ATCC 25923 and Lafferty as well as formalinized cells of these two strains and several clinical methicillin-resistant S. aureus (MRSA) isolates were immunogenic for New Zealand White rabbits. Rabbits which had recovered from experimental bacteremia due to MRSA seroconverted, i.e. demonstrated raised titers of antibodies against TA and PG of the S. aureus strain Lafferty and against whole cells (WC) and ultrasound cell lysates (UCL) of MRSA isolates No. 1 and 2 (representative of nosocomial MRSA strain I), as determind with enzyme-linked immunosorbent assays. Furthermore, sera from 2 long-term survivor rabbits recognized four polypeptides (apparent molecular weights = 38.9, 33.9, 30.9, and 28.2 kDa) shared by UCL extracts from MRSA isolates No. 1 and 2, as determined with immunoblots. Neither normal nor immune rabbit sera augmented the bactericidal activity of fresh defibrinated human blood (65% v/v) against selected MRSA isolates and S. aureus strain ATCC 25923. Sera from 12 patients with documented systemic infection due to MRSA outbreak strain I were examined for IgM and IgG antibodies against TA, WC, and UCL antigens. Three patient sera exhibited raised IgM antibodies against TA; 7 of 12 patient sera showed increased IgG anti-TA titers. Only 1 patient had a markedly raised IgM anti-WC titer, whereas 4 and 10 of the patients had increased IgG titers against WC from MRSA isolates No. 1 and 2, respectively. However, all 12 patients had raised IgG titers against UCL from MRSA isolate No. 2 versus 4 of 12 patients with elevated IgG titers against UCL from MRSA isolate No. 1. Immunoblots with 3 selected patient sera revealed IgG antibodies to be more multifaceted than IgM antibodies. Sera from 11 of the 12 patients contained antimicrobial drug(s); yet only 5 of these 11 sera (used at 10% v/v in broth) killed inocula of MRSA isolate No. 43. None of the 12 patient sera (10% v/v) enhanced the bactericidal activity of human blood against selected MRSA isolates. Neither three commercial intravenously applicable IgG preparations nor an IgG anti-α-hemolysin formulation (employed at 10% v/v) augmented the bactericidal activity of fresh defibrinated human blood against selected MRSA isolates comprising MRSA outbreak strain I.

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Evaluation of seven different enzyme-linked immunosorbent assays for serodiagnosis of Staphylococcus aureus bacteremia

Ryding

Espersen

Söderquist

et al. 2002

Diagnostic Microbiology and Infectious Disease

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Serological Diagnosis of Staphylococcal Bacteraemia

Cited by 6 publications

References 8 publications

Antibody response to Staphylococcus aureus whole cell, lipase and staphylolysin in patients with S. aureus infections

Antibody response to Staphylococcus aureus whole cell, lipase and staphylolysin in patients with S. aureus infections

Immunobiology of Methicillin-Resistant <i>Staphylococcus aureus</i>: Immune Response of Rabbits and Patients to Systemic Infection

Evaluation of seven different enzyme-linked immunosorbent assays for serodiagnosis of Staphylococcus aureus bacteremia

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