Serological Diagnosis of Dengue

Mosquito-Borne Dengue Fever-An Update

“…The major advance laboratory tools used for detecting dengue infection involve; (a) nucleic acid amplification tests (NAAT) to identify the specific virus serotype; (b) genomic sequences and viral isolation from mosquito cell lines and (c) ELISA to detect antigen and antibodies (Huang et al 2014) even though it is still the assay for immunity studies that is most frequently utilized (Lima et al 2022). In order to get around the limitations of PRNT, newer tests have been created such as the ELISA-based spot and microneutralization test, the fluorescent antibody cell sorter that based on dentritic cell specific intercellular adhesion of molecule-3-grab-bing Nonintegrin expressor DC assay.…”

Development and assessment of a multiepitope synthetic antigen for the diagnosis of Dengue virus infection

“… 13 However, there is conflicting evidence regarding the cross-reactivity of these tests with other flavivirus compromising their specific reliability. 14 Therefore, there is a need to develop cost-effective, safe, and simple diagnostic tests that combine sensitivity and specificity. The initial clinical evaluation is critical, as symptoms can mislead medical doctors, leading to incorrect treatment.…”