Serological Detection Of Enterotoxin From Food-Poisoning Strains Of Staphylococcus Aureus

Simkovicová, M

doi:10.1099/00222615-4-1-19

Cited by 62 publications

(25 citation statements)

References 23 publications

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“…Similar results were reported by Bergdoll et al (1982) when testing six strains isolated from food connected in food poisoning. No particular association between enterotoxigenicity and bacteriophage group has been reported, but staphylococci implicated in food poisoning are most likely to be lysed by phages ofgroup III (Simkovicova & Gilbert, 1971). Non-typable strains formed the largest group amongst our clinical and food poisoning strains (Tables 2 and 4) which supports the findings obtained by Payne & Wood (1974) while testing strains isolated from foods.…”

Section: Discussionsupporting

confidence: 80%

Enterotoxin production, phage typing and serotyping ofStaphylococcus aureusstrains isolated from clinical materials and food

Melconian¹,

Brun²,

Fleurette³

1983

J. Hyg.

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SUMMARYThe production of enterotoxins A, B, C and F by strains of Staphylococcus aureus isolated from various clinical sources and from isolates implicated in food poisoning was investigated.One hundred and ninety one of the 374 clinical strains (51-1 %o) were found to be enterotoxigenic; ofthese, 81 (27-7 %) strains produced enterotoxin A, 57 (15-3 %) strains produced enterotoxin B, 23 (6-2 00) strains produced enterotoxin C, and 64 (17-1 %) strains produced enterotoxin F. These enterotoxigenic strains were most frequently lysed by phages of group III (21P5 00) or were not typable (22 %).Eighteen of the 29 strains implicated in food poisoning were enterotoxigenic. The correlation of antigens and bacteriophage patterns with enterotoxigenicity was determined: enterotoxin A being related to a4 antigen, enterotoxin B to phages of 94/96 complex with cl, o antigens, and enterotoxin F to phages of group I with 2632, k1k2, m antigens.

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Section: Discussionsupporting

confidence: 80%

Enterotoxin production, phage typing and serotyping ofStaphylococcus aureusstrains isolated from clinical materials and food

Melconian¹,

Brun²,

Fleurette³

1983

J. Hyg.

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“…Each isolate was transferred to the Food Hygiene Laboratory where tests for production of enterotoxins A-E and TSST-1 were performed using the double gel diffusion technique [18]. Exfoliative toxins were not sought.…”

Section: Microbiological Methodsmentioning

confidence: 99%

“…Surely, there are clinical symptoms that can separate TSST-1 disease from SEB disease within TSS that can be defined. The association of SEB with burns patients has been suggested since the early Scandinavian studies [18].…”

Section: Toxic Shock Syndronmementioning

confidence: 99%

Enterotoxins and toxic-shock syndrome toxin-1 in non-enteric staphylococcal disease

Marples¹,

Wieneke

1993

Epidemiol. Infect.

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SUMMARYOver the 7 years 1985-91, 997 strains of Staphylococcus aureus from 962 patients with diseases other than food poisoning have been tested for the production of enterotoxins and toxic shock syndrome toxin-I (TSST-1) and phage typed. In all, 128 cases could be classified as confirmed or probable toxic shock syndrome (TSS) but a further 199 cases were classified as possible or unconfirmed TSS. In 219 cases, an alternative diagnosis could be supported and 45 cases were classified as sudden infant death syndrome. In 371 cases, insufficient information for classification was available.Strains of phage group I producing TSST-1 were associated with menstrual TSS. Many menstrual TSS cases were aged less than 20 and were using nonintroducer tampons.When all strains were reviewed, strong associations were observed between

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“…The enterotoxins and antisera of A, B and C were diluted in 0-02 M phosphate buffer in saline, pH 7*4, containing 0-02 % thiomersal, and those of D were diluted in Difco Brain-Heart Infusion (BHI) broth in saline. Towards the end of the work 10 % of BHI broth was added to the diluents used for enterotoxins A, B and C and their antisera to increase the sensitivity of the gel-diffusion test (Casman, Bennett, Dorsey & Stone, 1969 (Hobbs, Kendall & Gilbert, 1968) using a modified Miles & Misra (1938) Gilbert, 1971).…”

Section: Control Enterotoxins and Antiseramentioning

confidence: 99%

“…Slide gel double-diffusion test for enterotoxin Slight modifications were made to the method described by gimkoviiova & Gilbert (1971). Two test areas were prepared on an agar-coated slide between three parallel double layers of tape set 2 cm.…”

Section: Control Enterotoxins and Antiseramentioning

confidence: 99%

Serological detection of enterotoxin in foods implicated in staphylococcal food poisoning

1972

Self Cite

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SUMMARYTwo methods are described for the extraction of enterotoxin from foods incriminated in incidents of staphylococcal food poisoning. Enterotoxin was detected serologically in 12 of 24 food samples from 20 separate incidents: eight samples contained enterotoxin A, three contained D and one both A and B. The amount of enterotoxin in nine foods, based on 100 % recovery, varied from 0-02 to 0 09 ,tg./g.

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Serological Detection Of Enterotoxin From Food-Poisoning Strains Of Staphylococcus Aureus

Cited by 62 publications

References 23 publications

Enterotoxin production, phage typing and serotyping ofStaphylococcus aureusstrains isolated from clinical materials and food

Enterotoxin production, phage typing and serotyping ofStaphylococcus aureusstrains isolated from clinical materials and food

Enterotoxins and toxic-shock syndrome toxin-1 in non-enteric staphylococcal disease

Serological detection of enterotoxin in foods implicated in staphylococcal food poisoning

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