Serological characterization of viruses isolated from experimental mucosal disease

Shimizu, Mitsugu; Satou, Kunio; Nishioka, Nobuyoshi; Yoshino, Tomoo; Momotani, Eiichi; Ishikawa, Yoshiharu

doi:10.1016/0378-1135(89)90087-4

Cited by 32 publications

(25 citation statements)

References 10 publications

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“…For the titration of cp-BVDV, the plaques were counted by eye under the microscope after 2 days. For the titration of ncp-BVDV, an agarose overlay was used, and plaques were counted by peroxidase immunostaining after hybridization with an anti-BVDV antibody and a secondary antibody conjugated with peroxidase, as described previously (39). The 50% and 90% inhibitory concentrations (IC 50 and IC 90 ) were determined as the concentrations at which the number of plaques was halved or reduced by 90%, respectively, compared to untreated infected control cells.…”

Section: Methodsmentioning

confidence: 99%

Effects of Interferon, Ribavirin, and Iminosugar Derivatives on Cells Persistently Infected with Noncytopathic Bovine Viral Diarrhea Virus

Durantel

Carrouée-Durantel

Branza‐Nichita

et al. 2004

Antimicrob Agents Chemother

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Persistent infection with hepatitis C virus (HCV) is a major cause of chronic hepatitis in humans. In chronic carriers, the viral infection induces liver damage that predisposes the patient for cirrhosis and can lead to hepatocellular carcinoma. Current chemotherapies are limited to alpha interferon (IFN-␣) used either alone or in combination with ribavirin (RBV). In addition to its limited efficacy, this treatment is frequently poorly tolerated because of its side effects. The urgently needed development of new drugs is made difficult by the lack of an in vitro or in vivo infectivity model, and no cell line has been found so far to reliably and reproducibly support HCV infection. For this reason, the closely related pestivirus bovine viral diarrhea virus (BVDV) has sometimes been used as a surrogate in vitro infectivity model. In this study we used an MDBK cell line persistently infected with noncytopathic BVDV to assess the antiviral effect of IFN-␣ and RBV, the two drugs currently in clinical use against HCV. The same system was then used to evaluate the potential of two classes of iminosugar derivates to clear noncytopathic BVDV infection from MDBK cells. We show that treatment with long-alkyl-chain deoxynojirimycin derivatives, which are inhibitors of the endoplasmic reticulum (ER)-resident ␣-glucosidases, can greatly reduce the amount of secreted enveloped viral RNA. Long-alkyl-chain deoxygalactonojirimycin derivatives, which do not inhibit ER ␣-glucosidases, were less potent but still more effective in this system than IFN-␣ or ribavirin. Hepatitis C virus (HCV) causes persistent infection in ap-proximately 70 to 80% of people infected and is responsible for liver injuries that can lead to cirrhosis and hepatocellular carcinoma (2, 34). While current therapeutic strategies against HCV infection rely mainly on either naked or pegylated alpha interferon (IFN-␣) alone or in combination with ribavirin (RBV) (19,28,34,37,43), considerable efforts are being made to develop new molecules which show better efficacy, particularly for refractory patients, who represent on average 50% of patients treated (19,28,34,42,43). Depending on the HCV genotype, the percentage of refractory patient varies from 80% (genotype 1b) to 20% (genotype 3).Molecules targeting viral activities, such as protease-or polymerase-specific inhibitors, are traditionally the most attractive candidates for drug development, though this strategy faces resistance problems, especially when dealing with fastmutating viruses like HCV and human immunodeficiency virus. The problem of drug-resistant viral escape mutants has also been observed for hepatitis B virus (13,18,35,36). Alternatively, a host cell target could be chosen which would have to be more important for the survival of the virus than for that of the host. Such a target would arguably be more difficult for the virus to mutate around.The feasibility of such an approach has recently been investigated with deoxynojirimycin (DNJ)-based iminosugar derivatives as antiviral agents (6,7,17,29,30...

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Section: Methodsmentioning

confidence: 99%

Effects of Interferon, Ribavirin, and Iminosugar Derivatives on Cells Persistently Infected with Noncytopathic Bovine Viral Diarrhea Virus

Durantel

Carrouée-Durantel

Branza‐Nichita

et al. 2004

Antimicrob Agents Chemother

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“…BVDV is spread widely among cattle populations in every part of the from their blood. The antigenicity of the isolated virus was closer to that of the Nose strain [12] than to the KS86-1 (+) strain [17].…”

Section: Animalsmentioning

confidence: 96%

“…12, KS86-1 (+) and KS86-1 (-) strains were used for antiviral assays of cytokines. Nose and KS86-1 (+), isolated from cattle with clinical symptoms of mucosal disease, are cytopathogenic (CP) strains [12,17]. No.…”

Section: Animalsmentioning

confidence: 99%

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Anti-Viral Effect of Interferon-.ALPHA. on Bovine Viral Diarrhea Virus.

Sentsui

Takami

Nishimori

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. To get basic information to control persistent virus infection among domestic animals by cytokines, the antiviral activity of four natural human cytokines against bovine viral diarrhea virus (BVDV) was evaluated. Normal bovine peripheral blood mononuclear leukocytes (PBML) and fetal bovine muscular cells (FBMC) were treated with varying doses of human interferon (IFN)-α, IFN-γ, tumor necrosis factor (TNF)-α and TNF-β. The antiviral activity in treated cells was measured by the titration of virus infectivity in comparison with non-treated controls. IFN-α significantly suppressed virus growth in both PBML and FBMC. The growth of two cytopathogenic and two noncytopathogenic strains was suppressed in the presence of more than 10 3 u/ml of IFN-α. Addition of either TNF-α or TNF-β to IFN-α did not potentiate the suppressive effect. IFN-α also suppressed the replication of BVDV in PBML from cattle persistently infected with BVDV. -KEY WORDS: bovine viral diarrhea virus, cytokine, interferon, persistent infection, pestivirus.

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“…The titer of NCP BVDV was determined with an ordinary 50% tissue culture infectious dose (TCID 50 ) on microtiter plates. The titer of the NCP BVDV was measured by an interference test in which the cytopathogenic (CP) Nose strain was used as the challenge virus [16]. Bovine fetal muscular (BFM) cell cultures were used for NCP BVDV isolation and titration.…”

Section: Collection Of Virus Samples During In Vitro Culture and Virumentioning

confidence: 99%

Bovine Viral Diarrhea Virus Replication in Bovine Follicular Epithelial Cells Derived from Persistently Infected Heifers.

Tsuboi

Imada

1998

The Journal of Veterinary Medical Science

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ABSTRACT. Bovine follicle fluid and oocytes surrounded by follicular epithelial (FE) cells were collected from ovaries of two heifers persistently infected with bovine viral diarrhea virus (BVDV). BVDV was present in the follicle fluid at a higher titer than in serum. The oocytes were matured in vitro under culture conditions of 39°C in humidified air containing 5% CO 2 . In vitro fertilization was performed after 24 hr in culture (the day of insemination was defined as day l), and culture was continued through day 10. BVDV was present in the culture medium at titers of 10 2.25 to 10 3.25 TC I D 50 /0.l ml. The virus was also detected in FE cells collected on day 10. Viral antigen was demonstrated in the cytoplasm of FE cells by the indirect immunofluorescence technique. However, no BVDV was detected in the embryos on day 10. These findings suggested that the oocytes or embryos were unlikely to be infected with BVDV, but that the FE cells were infected with BVDV and supported virus replication in cattle persistently infected with BVDV. -KEY WORDS: bovine viral diarrhea virus, follicular epithelial cell, follicular fluid, in vitro fertilization, persistent infection.

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Serological characterization of viruses isolated from experimental mucosal disease

Cited by 32 publications

References 10 publications

Effects of Interferon, Ribavirin, and Iminosugar Derivatives on Cells Persistently Infected with Noncytopathic Bovine Viral Diarrhea Virus

Effects of Interferon, Ribavirin, and Iminosugar Derivatives on Cells Persistently Infected with Noncytopathic Bovine Viral Diarrhea Virus

Anti-Viral Effect of Interferon-.ALPHA. on Bovine Viral Diarrhea Virus.

Bovine Viral Diarrhea Virus Replication in Bovine Follicular Epithelial Cells Derived from Persistently Infected Heifers.

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