Serological and molecular investigation of Ehrlichia spp. and Anaplasma spp. in ticks and blood of dogs, in the Thrace Region of Turkey

Çetinkaya, Handan; Matur, Erdal; Akyazı, İbrahim; Ekiz, Elif Ergül; Aydın, Levent; Toparlak, Müfit

doi:10.1016/j.ttbdis.2016.02.021

Cited by 50 publications

(32 citation statements)

References 78 publications

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“…The positive results we found support previous reports of dogs as a host for A. phagocytophilum (Woldehiwet, 2010;Atif, 2015;Çetinkaya et al, 2016). The previous report of A. phagocytophilum in dogs by PCR worldwide (serological studies excluded) vary from 0.5 to 6.3% (Çetinkaya et al, 2016), which is much less than the 27% prevalence we found.…”

Section: Discussionsupporting

confidence: 88%

“…The previous report of A. phagocytophilum in dogs by PCR worldwide (serological studies excluded) vary from 0.5 to 6.3% (Çetinkaya et al, 2016), which is much less than the 27% prevalence we found. This difference could be due to: (1) A. phagocytophilum strain, (2) the presence of this pathogen in the rest of the vertebrate host community in the study area, and (3) previous studies have found that dogs with access to veterinary care might have low prevalence values, opposite to our study area (Jensen et al, 2007;Bowman et al, 2009;Çetinkaya et al, 2016;Movilla et al, 2016). We need further investigation to support the first two hypotheses and to discard that the high prevalence are due to the low sample size.…”

Section: Discussionmentioning

confidence: 66%

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Infection of Anaplasma phagocytophilum and Ehrlichia spp. in Opossums and Dogs in Campeche, Mexico: The Role of Tick Infestation

2017

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In recent years, some tick-borne diseases such as anaplasmosis and ehrlichiosis became widespread worldwide, threatening the health of humans, domestic animals and wildlife. The aims of this study were to determine the presence of Anaplasma phagocytophilum, Ehrlichia canis, and Ehrlichia chaffeensis in 102 opossums (Didelphis spp.) and 44 owned free-ranging dogs in southeastern Mexico using a specific polymerase chain reaction (PCR). A. phagocytophilum was detected in opossums and dogs with a prevalence of 3 and 27%, respectively. E. canis was only present in 7% of dogs, while we didn't detect E. chaffeensis in any host. We report the first evidence of infections of A. phagocytophilum in Didelphis virginiana and D. marsupialis in Mexico. The infection rates and patterns we found of A. phagocytophilum suggest that dogs are more directly involved in the ecology of this pathogen than opossums. Despite the small prevalence found, our results are of public health concern because of the zoonotic capabilities of A. phagocytophilum, the high tick infestation rates found and because both opossums and free-ranging dogs can achieve high population densities in the region.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 66%

Infection of Anaplasma phagocytophilum and Ehrlichia spp. in Opossums and Dogs in Campeche, Mexico: The Role of Tick Infestation

2017

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“…Several surveys have previously suggested high exposure rates in other Mediterranean countries, including Spain (3%‐55%), Italy (7%‐47%), Portugal (4%‐26%), and Tunisia (54%) . High seroprevalence has also been reported in countries bordering Greece, including Albania (21%), Bulgaria (21%‐38%), and Turkey (21%‐28%) . In contrast, E canis seroreactivity declines precipitously in countries north of the Mediterranean basin (0.5%‐10%) .…”

Section: The Associations Between the Presence Of Anti‐ehrlichia Canimentioning

confidence: 99%

Seroprevalence and risk factors associated with Ehrlichia canis in a hospital canine population

Chochlios

Angelidou

Kritsepi‐Konstantinou

et al. 2019

Veterinary Clinical Pathol

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Ehrlichia canis (E canis) is the principal cause of canine monocytic ehrlichiosis (CME) and the only Ehrlichia spp that has been identified in the dogs of Europe. 1 Clinical recovery is the expected outcome of acutely infected dogs entering the subclinical phase, which could last several months to years; however, an unpredictable proportion of dogs will eventually develop the chronic phase, typically characterized by aplastic pancytopenia and a high mortality. 2 Fever, depression, anorexia, lymphadenomegaly, splenomegaly, mucosal pallor, ocular abnormalities, bleeding tendencies, and thrombocytopenia or bi-pancytopenia are common clinical and hematologic findings in CME. 1,2 The indirect fluorescent antibody (IFA) test and several in-clinic assays have enabled the comprehensive evaluation of exposure to E canis in dogs living in or traveling across endemic areas. 3 Several Abstract Background: Ehrlichia canis (E canis) infection has been documented in a few small canine case series in Greece. However, there is limited information on the prevalence of exposure to, or the potential risk factors associated with E canis seroreactivity in a large native canine population. Objectives:The objectives of this study were to evaluate E canis seroprevalence in dogs admitted to a veterinary teaching hospital, and to investigate the potential association between seropositivity and signalment, health status, the serologic assays used, and selected clinical and clinicopathologic abnormalities. Methods:The medical records of 850 client-owned dogs, tested using three in-office serologic assays, were retrospectively reviewed. Results:The E canis seroprevalence was significantly higher in sick (54.9%) compared with healthy (33.9%) dogs. Seropositivity differed significantly between the serologic assays used in this study (ImmunoComb vs SNAP 3Dx/SNAP 4Dx). Dogs presenting with bleeding tendencies, anemia, thrombocytopenia, leukopenia, pancytopenia, and hyperproteinemia were more likely to be E canis seropositive, and the median hematocrit (HCT), white blood cell (WBC), neutrophil, and platelet values were significantly lower in seropositive compared with seronegative dogs. Conclusions:A high E canis seroprevalence was documented in a canine population living in an endemic area. Selected clinicopathologic variables might be useful indicators of E canis exposure and could allow the prioritization of serologic testing in the clinical setting. K E Y W O R D Sdog, monocytic ehrlichiosis, serology

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“…To date, two large Babesia species, namely B. canis and B. vogeli and one small Babesia species, B. gibsoni have been identifi ed in dogs [16,17,[19][20][21][22][23]. Canine monocytic erhlichiosis caused by E. canis was also reported in Turkey [16,17,24,25].…”

Section: Introductionmentioning

confidence: 99%

A Retrospective Epidemiological Study: The Prevalence of Ehrlichia canis and Babesia vogeli in Dogs in the Aegean Region of Turkey

et al. 2019

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Among tick-borne diseases, Ehrlichia canis and Babesia piroplasm cause important diseases in dogs where the distributions of the pathogen, vector and host overlap. The primary aim of the present study was to detect the prevalence of Babesia spp. and E. canis using PCR and reverse line blot (RLB) hybridization assay in a total of 379 samples comprising stray and owned dogs and to compare the diagnostic sensitivity of the two tests. Overall, 41.4% of dogs were infected with B. vogeli and/or E. canis as single (35.4%) and mixed (6.1%) infections. The majority of Babesia positive dogs (74.1%) were co-infected with E. canis. PCR detected a higher (P= 0.000) number of positivity in some provinces compared to RLB. To the best of our knowledge, these findings provide the first molecular evidence for the existence of B. vogeli in the Aegean Region, Turkey. The present study pinpoints the distribution and prevalence of E. canis and B. vogeli in the Aegean region of Turkey as of 2004 and as such establishes a baseline. This is of pivotal importance for future studies aimed to demonstrate changes in the dynamics of E. canis and B. vogeli infections in the region.

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Serological and molecular investigation of Ehrlichia spp. and Anaplasma spp. in ticks and blood of dogs, in the Thrace Region of Turkey

Cited by 50 publications

References 78 publications

Infection of Anaplasma phagocytophilum and Ehrlichia spp. in Opossums and Dogs in Campeche, Mexico: The Role of Tick Infestation

Infection of Anaplasma phagocytophilum and Ehrlichia spp. in Opossums and Dogs in Campeche, Mexico: The Role of Tick Infestation

Seroprevalence and risk factors associated with Ehrlichia canis in a hospital canine population

A Retrospective Epidemiological Study: The Prevalence of Ehrlichia canis and Babesia vogeli in Dogs in the Aegean Region of Turkey

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