Serological and molecular detection of tobacco veinal necrosis isolates ofPotato virus Y (PVYN) from potatoes grown in the Western United States

Crosslin, J. M.; Hamm, Philip B.; Shiel, P. J.; Hane, D. C.; Brown, Charles R.; Berger, P. H.

doi:10.1007/bf02871955

Cited by 37 publications

(20 citation statements)

References 22 publications

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“…The PVA isolate was collected from field infected Russet Burbank and was used because resistance to PVA has been shown to be tightly linked to the Ry adg gene (6.8 cM distal to the gene) with concurrent expression of resistance to both PVY and PVA having been documented (Hamalainen et al 1998). The PVY N isolate (designated RR-1) from field infected Ranger Russet was verified to strain identity by Crosslin et al (2005). To perform the graft, a 10 cm tall stem was decapitated 4 cm below the growing point.…”

Section: Graft Challenge Of Select Breeding Program Clones and Cultivarsmentioning

confidence: 99%

Characterization of Broad Spectrum Potato Virus Y Resistance in a Solanum tuberosum ssp. andigena-Derived Population and Select Breeding Clones Using Molecular Markers, Grafting, and Field Inoculations

et al. 2009

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PVY causes yield and quality loss in potato. The Ry adg gene from Solanum tuberosum ssp. andigena has been shown to provide extreme resistance to PVY; defined as resistance against all strains. However, Ry adg gene clones have not been screened against PVY N:O , a newly detected North America strain. Three Ry adg -diagnostic molecular markers were tested in tetraploid progeny of a PVY resistant x susceptible cross and in diverse clones/cultivars. Multiple isolates of PVY strains (PVY NTN , PVY N:O , PVY O ) were used for mechanical inoculations of the progeny. In addition, PVY O , PVY N , and PVA were used for graft inoculation on a separate clone/cultivar set. Progeny segregated 1:1 for PVY resistance; fitting a gene model simplex for Ry adg . Marker positive progeny were resistant to all PVY strains, including PVY N:O . Marker presence was also in agreement with PVY resistance in the clones/cultivars. These findings show that these markers can identify resistance to all known PVY strains in North America.Resumen El virus Y de la papa (PVY) causa reducción en la calidad y el rendimiento. Se ha demostrado que el gen Ry adg de Solanum tuberosum ssp. andigena provee resistencia extrema contra el PVY; definida como resistencia contra todas las cepas. Sin embargo, no se ha investigado la resistencia de los clones del gen Ry adg contra PVY N:O , una cepa de Norte América recientemente detectada. Tres marcadores moleculares de diagnostico del gen Ry adg fueron probados en progenie tetraploide de un cruce de PVY resistente x susceptible y en diversos clones/cultivares. Múltiples cepas aisladas de PVY (PVY NTN , PVY N:O , PVY O ) fueron utilizadas para inoculaciones mecánicas de la progenie y PVY O , PVY N y PVA fueron usados para inoculación de injertos en un subconjunto de clones/ cultivares. La progenie segregó 1:1 para resistencia contra PVY; encajando en un modelo genético simplex para Ry adg . Las progenies positivas a los marcadores fueron resistentes a todas las cepas de PVY, incluyendo PVY N:O . La presencia de marcadores tambien concordaba con la resistencia contra PVY en clones/cultivares. Estos hallazgos demuestran que estos marcadores pueden identificar la resistencia a todas las cepas de PVY conocidas en Norte América.

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Section: Graft Challenge Of Select Breeding Program Clones and Cultivarsmentioning

confidence: 99%

Characterization of Broad Spectrum Potato Virus Y Resistance in a Solanum tuberosum ssp. andigena-Derived Population and Select Breeding Clones Using Molecular Markers, Grafting, and Field Inoculations

et al. 2009

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“…The necrotic strains were until recently on the list of quarantine pests for the North American Plant Protection Organization (NAPPO) countries (Canada, Mexico, and U.S.) (Anonymous 2003) and detection often resulted in regulatory actions and suspension of cross-border trade. Numerous reports of necrotic strains present in field grown potatoes (Baldauf et al 2006;Crosslin et al 2006;Crosslin et al 2005;Karasev et al 2008;Lorenzen et al 2006a;Nie and Singh 2003;Nie et al 2004;Piche et al 2004;Xu 2008;Xu et al 2005) and the adoption of a US-Canada Binational Tuber Necrotic Virus Management Plan in 2006 has resulted in the removal of necrotic strains of PVY from the quarantine list. Currently the entire PVY complex is managed primarily by seed certification standards.…”

Section: Introductionmentioning

confidence: 97%

Serological Properties of Ordinary and Necrotic Isolates of Potato virus Y: A Case Study of PVYN Misidentification

Karasev

Николаева

et al. 2009

Am. J. Pot Res

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In the course of a multi-year survey of Potato virus Y (PVY) incidence and diversity in the U.S. seed potato crop, an unusual PVY variant was identified in low but significant levels in multiple states. This variant, PVY O -O5, was initially detected by a commercially available PVY N -specific monoclonal antibody, 1F5. This antibody is widely used by U.S. Seed Certification programs to test for PVY N and is one of two antibodies designated by the North American Plant Protection Organization (NAPPO) for preshipment testing of tuber lots that are to be transported between countries. Consequently, PVY N positives identified by the 1F5 antibody have triggered quarantine actions, prevented cross-border shipments and impacted trade. Here, we demonstrate by a variety of methods that the PVY O -O5 is a variant within the ordinary PVY strain (PVY O ). Specifically, the PVY O -O5 variant likely arose due to a single amino acid substitution within the capsid protein. This variant does not induce vein necrosis in tobacco or tuber necrosis in susceptible varieties of potato. Furthermore, it is identified by RT-PCR based diagnostics as PVY O and it has a typical PVY O genome sequence. We demonstrate that another PVY N specific monoclonal antibody, SASA-N, recognizes an epitope distinct from that recognized by 1F5, and correctly identifies the PVY O -O5 variants as belonging to the PVY O serotype. Since the PVY O -O5 variant is present in many seed producing states and misidentification of PVY O -O5 as PVY N/NTN has clear quarantine implications for export shipments of potato, the limitations of the commercially available monoclonal antibodies should be considered in any certification or phytosanitary testing program.Resumen A lo largo del estudio de varios años sobre la incidencia y diversidad del virus Y de la papa (PVY) en los cultivos de papa para semilla en los Estados Unidos (EU), se identificó a una variante inusual a niveles bajos pero significativos en múltiples estados. Esta variante, PVY O -O5, se detectó inicialmente con un anticuerpo monoclonal comercialmente disponible específico para PVY N , el 1F5. Este anticuerpo es ampliamente usado por los Programas de Certificación de Semilla en los EU para PVY N , y es uno de los dos anticuerpos designados por la Organización Norteamericana de Protección de Plantas (NAPPO) para pruebas de pre-envío de lotes de tubérculos que serán transportados entre países. Consecuentemente, los PVY N positivos identificados con el anticuerpo 1F5 han disparado acciones cuarentenarias, evitando envíos trans-fronteras y han impactado al comercio. Aquí, nosotros demostramos con diversos métodos que PVY O -O5 es una variante del PVY ordinario (PVY O ). Específicamente, la variante PVY O -O5 es probable que haya surgido debido a una substitución de un aminoácido dentro de la proteína de la cápside. Esta variante no induce necrosis de las venas en tabaco o necrosis del A. V. Karasev : O. V. Nikolaeva : X. Hu : Z. Sielaff : tubérculo en variedades susceptibles de papa. Aún mas, se le identifica c...

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“…Some of them have been maintained as stock "cultures" for many years. These included the PVY isolates which have been used in numerous experiments (Crosslin et al 2005;2006a, b;Lorenzen et al 2006). Similarly, the Tobacco rattle virus (TRV) infected tobacco (cv.…”

Section: Methodsmentioning

confidence: 99%

“…The enzyme-linked immunosorbent assay (ELISA), in particular, has been widely adopted for this purpose (Clark and Adams 1977;Crosslin et al 2005). More recently, however, the reverse transcription polymerase chain reaction (RT-PCR) has been adopted for detection of many viruses and viroids due to its specificity and sensitivity (Singh 1999b;Robinson 1992;Candresse et al 1998).…”

Section: Introductionmentioning

confidence: 97%

Standardized RT-PCR Conditions for Detection and Identification of Eleven Viruses of Potato and Potato spindle tuber viroid

Crosslin

Hamlin

2011

Am. J. Pot Res

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Serological and molecular detection of tobacco veinal necrosis isolates ofPotato virus Y (PVYN) from potatoes grown in the Western United States

Cited by 37 publications

References 22 publications

Characterization of Broad Spectrum Potato Virus Y Resistance in a Solanum tuberosum ssp. andigena-Derived Population and Select Breeding Clones Using Molecular Markers, Grafting, and Field Inoculations

Characterization of Broad Spectrum Potato Virus Y Resistance in a Solanum tuberosum ssp. andigena-Derived Population and Select Breeding Clones Using Molecular Markers, Grafting, and Field Inoculations

Serological Properties of Ordinary and Necrotic Isolates of Potato virus Y: A Case Study of PVYN Misidentification

Standardized RT-PCR Conditions for Detection and Identification of Eleven Viruses of Potato and Potato spindle tuber viroid

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