Serological and Molecular Detection of Avian Pneumovirus in Chickens with Respiratory Disease in Jordan

Gharaibeh, Saad; Algharaibeh, G. R.

doi:10.1093/ps/86.8.1677

Cited by 18 publications

(18 citation statements)

References 18 publications

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“…Out of 347 serum samples obtained from broiler chickens, 167 (48.1%) were positive to aMPV antibodies, which represented 20 (83.3%) of 24 examined broiler flocks. Gharaibeh and Algharaibeh (2007) reported that out of 38 chicken flocks in Jordan tested by ELISA, 18 locks (47.4%) were found to have positive antibody titer for aMPV, the positive flocks comprised 21.7, Goyal et al (2003) reported that the average seroprevalence rate of aMPV in Minnesota turkeys was 36.3%. A higher rate of seropositivity (83.3% of samples and 100% of broiler flocks) was observed in NW; this area had the highest concentration of broiler flocks.…”

Section: Discussionmentioning

confidence: 99%

“…In Poland, Minta et al (1995) used ELISA to detect seroprevalence to avian pneumovirus in sera collected from 39 broiler breeder flocks aged 12-96 weeks, 56.4% of broiler breeder flocks were positive. Gharaibeh and Algharaibeh (2007) reported that 100% of broiler breeder flocks in Jordan tested by ELISA were found to have positive antibodies to aMPV.…”

Section: Discussionmentioning

confidence: 99%

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Seroprevalence of avian metapneumovirus infection in broiler and broiler breeder chickens in Iran

Rahimi¹

2011

Vet. Med. - Czech

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Avian metapneumovirus causes an acute highly contagious upper respiratory tract infection primarily of turkeys and chickens. The disease can cause significant economic losses in turkey and chicken flocks, particularly when exacerbated by secondary pathogens. The purpose of this study was to determine the prevalence of avian metapneumovirus antibodies in broiler and broiler breeder flocks in Kermanshah province, west of Iran. All the flocks had not been vaccinated against avian metapneumovirus. The province were divided into four geographic areas; southwest, southeast, northwest, and northeast. Flocks in each area, and 14-15 birds in each flock, were randomly sampled. The blood samples were taken regardless of the presence of any signs of respiratory or any other clinical disease in the flocks. A total of 435 blood samples were collected from 30 commercial chicken flocks (24 broiler flocks, aged between six and eight weeks, and six broiler breeder flocks, aged between 56 and 72 weeks). The presence of antibodies against avian metapneumovirus in each serum sample was tested twice by enzyme-linked immunosorbent assay using a commercial kit which was able to determine antibodies against A, B and C subtypes of avian metapneumovirus. Out of 347 serum samples obtained from broiler chickens, 167 (48.1%) were positive to avian metapneumovirus antibodies, which represented 20 (83.3%) of 24 examined broiler flocks. Out of 88 samples obtained from broiler breeder chickens, 82 (93.2%) were positive to avian metapneumovirus antibodies, which belonged to six (100%) of examined broiler breeder flocks. Detection of anti-avian metapneumovirus antibodies among broiler breeder (100%) was higher than broiler (83.3%) flocks. A higher rate of seropositivity (83.3% of samples and 100% of broiler flocks) was observed in northwest. The results of this study may indicate the possible involvement of avian metapneumovirus in the respiratory disease we are seeing in chickens in Iran. Its prevalence has to be investigated in other parts of Iran. Future work may and should include the use of molecular methods and isolation of the virus. Isolation of avian metapneumovirus will allow the possibility of making autogenous vaccines.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Seroprevalence of avian metapneumovirus infection in broiler and broiler breeder chickens in Iran

Rahimi¹

2011

Vet. Med. - Czech

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“…12 We have documented the presence of aMPV subtype B in Jordanian chicken flocks. 8 In this study, we investigate the susceptibility of pigeons and sparrows to this subtype B aMPV and their possible role in transmission among chicken farms.…”

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Avian Metapneumovirus Subtype B Experimental Infection and Tissue Distribution in Chickens, Sparrows, and Pigeons

Gharaibeh

Shamoun

2011

Vet Pathol

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Avian metapneumovirus (aMPV) is a respiratory virus that infects a range of avian hosts, including chickens and turkeys. Migratory and local wild birds are implicated in aMPV spread among farms, countries, and seasonal outbreaks of the disease. A subtype B aMPV isolate from commercial chicken flocks suffering from respiratory disease was experimentally inoculated oculonasally into 7-week old chickens, young pigeons, and sparrows. Chickens showed minimal tracheal rales, whereas pigeons and sparrows were asymptomatic. Shedding of aMPV was detected by reverse transcription polymerase chain reaction on homogenates from nasal turbinates. At 5 days postinfection, 5 of 5 chickens, 2 of 5 pigeons, and 1 of 5 sparrows were positive; at 10 or 15 days, none were positive. At 2 and 5 days, aMPV antigens were localized at the ciliated boarder of respiratory epithelium in nasal cavity and trachea of chickens, as well as to the conjunctival epithelium. Pigeons had detectable viral antigens in only the trachea at 2 and 5 days; sparrow tissues did not show any positive staining. At the end of the experiment, at 21 days postinfection, 14 of 15 inoculated chickens seroconverted against aMPV, but none of the inoculated pigeons or sparrows did. The authors believe that pigeons and sparrows have the ability to transmit the virus between chicken farms, although they do not consider pigeons and sparrows as natural hosts for aMPV, given that they failed to seroconvert. In conclusion, pigeons and sparrows are partially susceptible to aMPV infection, probably acting more as mechanical vectors because infection is only temporary and short-lived.

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“…Virus isolation can be performed in tracheal organ cultures, chicken embryo fibroblasts, or Vero cells, but it is time-consuming and often unsuccessful [11]. Avian metapneumovirus RNA can be detected for a short period (2 to 10 d postinfection) by reverse-transcription polymerase chain reaction in tracheal and choanal swabs [12,13]. Antibodies to AMPV are detectable for many weeks by enzyme-linked immunosorbent assay (ELISA), which is more rapid and economical than virus isolation or reverse-transcription polymerase chain reaction [14][15][16].…”

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Serological survey of antibodies against avian metapneumovirus in Korean chicken flocks

Park¹,

Lee²,

Shin³

2011

Journal of Applied Poultry Research

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Laboratory tests to identify the viral genome, the antigen, or specific antibodies are used for a definitive diagnosis of avian metapneumovirus (AMPV) infection. Methods that can be used for antibody determination are the virus neutralization test and the enzyme-linked immunosorbent assay. To monitor the presence of AMPV in Korean chickens, sera were taken from chickens in 20 flocks. Of the 197 serum samples tested by enzyme-linked immunosorbent assay, 144 (73.1%) were positive for AMPV. The incidence of AMPV antibodies was higher in broilers (97.3%; 36 of 37 chickens) than in layers (67.5%; 108 of 160 chickens). Age was a factor, with very few positive samples obtained from young birds and the highest positive rates obtained for birds more than 100 d old.

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Serological and Molecular Detection of Avian Pneumovirus in Chickens with Respiratory Disease in Jordan

Cited by 18 publications

References 18 publications

Seroprevalence of avian metapneumovirus infection in broiler and broiler breeder chickens in Iran

Seroprevalence of avian metapneumovirus infection in broiler and broiler breeder chickens in Iran

Avian Metapneumovirus Subtype B Experimental Infection and Tissue Distribution in Chickens, Sparrows, and Pigeons

Serological survey of antibodies against avian metapneumovirus in Korean chicken flocks

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