Serological and Immunohistochemical Detection of Helicopacter pylori Infection among Egyptian Hepatitis C Virus Patients

Abstract

Background and study aim: Helicobacter pylori infection is distributed worldwide. H. pylori colonize the liver and increase the severity of the liver pathogenesis. The aim of the present study was to detect the H. pylori in the HCV patients using serology and immunohistochemistry diagnostic methods. The aim was extended to evaluate the hematological and biochemical changes during the co-infection. Materials and Methods: ELISA and immunohistochemistry diagnostic methods were utilized to examine patients chronically infected with HCV for H. pylori, and some hematological and biochemical parameters were detected. Results: Overall prevalence of H. pylori infection was 10.81%, 8.00%, using ELISA, and immunohistochemistry, respectively. In the current study, no significant difference based on gender, residence, age, biochemical assessment and HCV RNA load was observed. Liver cirrhosis at patient coinfected with H. pylori and HCV recorded high percentage compared to those with chronic HCV mono-infection. A significant increase in the relative lymphocyte count was detected in patients with concomitant H. pylori and chronic HCV infections compared to patients with chronic HCV mono-infection. Conclusion: Because H. pylori infection is frequent among Egyptian HCV infected patients, regular screening and treatment for H. pylori among this category is extremely important.

Value of Helicobacter pylori Eradication on Platelet Count in chronic Hepatitis C Virus-Infected Patients with thrombocytopenia

“…In our study, H. pylori infection recorded prevalence among HCV patients with thrombocytopenia 65%( 65/100) versus 35% (35/100) H. pylori negative this agreement with Takashima et al (2016) 1 The research included sixty-five patients with HCVrelated CLD and thrombocytopenia, of which 30 (46%) were H. pylori-positive. in contrast to Ibrahim et al, (2018) 9 Using ELISA and IHC, respectively, the average incidence of H. pylori was 10.81 % and 8.00 %. Aljarad S et al, (2018) 10 and Lee et al, (2020) 11 reported the frequency of Helicobacter pylori infection was 72% and 54.3% respectively in ITP patients in contrast to Sultan et al, (2016) 12 show frequency of Helicobacter pylori infection in ITP 11.1%.…”

Serological and Immunohistochemical Prevalence of Human Cytomegalovirus Co-infection among Hepatitis C Virus Patients Admitted to Kafer El Shiekh Liver and Heart Institute, Egypt

“…Agreement between ELISA and immunohistochemistry was calculated according to Ibrahim et al [34][35][36][37].…”