Serological and genetic analysis of ABO ambiguous blood group samples

Xu, Jun; Zhu, Yu; Xiong, Ting; Zhao, Ling; Yao, Yun; Zhou, Xiaoyu

doi:10.46701/bg.2020022020112

Cited by 1 publication

(1 citation statement)

References 5 publications

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“…The ratio of the ABO subgroup is about 0.047% in Chinese [2] . The identification of ABO subgroups requires additional experiments to assist in the interpretation [3] , as forward and reverse ABO typing are inconsistent among ABO subgroup samples [4] . Therefore, genetic analyses of ABO coding genes, as well as molecular typing are required when ABO subgroups are encountered, where serological typing could not determine the sample blood type.…”

Section: Discussionmentioning

confidence: 99%

Serological and genetic analysis of a rare CisAB01/O01 blood group

Liu¹,

Yi²,

Gao³

et al. 2021

Blood and Genomics

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The paper aims to analyze a rare blood sample in Ganzhou City Hospital with CisAB subtype and explore a feasible pattern for blood typing of rare blood type patients, so as to ensure clinical transfusion safety. The routine serological methods were used for ABO forward and reverse blood typing and the fluorescence real-time PCR technique was used for sample genotyping. A human ABO blood group 6-7 exon sequencing kit was used for sequence analysis. The nucleic acid sequence of the sample was compared with reference sequences. The forward typing results demonstrated that the sample was ABw, RhD positive. The sample exhibited 4+ agglutination with anti-H and anti-AB antibodies. Reverse typing by microcolumn gel method showed an AB result, but the serum sample demonstrated weak agglutination with B cell under room temperature, 4 °C and 37 °C in saline when tested with tube method respectively. The serological results matched with the A 2 B 3 serotype. The fluorescent realtime PCR genotyping results displayed A/O01. The sequence analysis demonstrated deletion of guanine in 261position 467C>T (heterozygote) and 803G>T (heterozygote) mutation respectively. The mutation caused the A glycosyltransferase peptide chain to change from proline to leucine (P156L) at 156 and from glutamate to alanine (G268A) at 268. The result demonstrated that the sample's genotype was CisAB01/O01. The mutation of glycosyltransferase coding gene leads to an abnormal serological reaction pattern. Only by combining the results of genetic analysis can we get the true sample blood type and better ensure the safety of clinical blood transfusion.

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Section: Discussionmentioning

confidence: 99%