Serological and ecological studies ofRhizobium spp. (Cicer arietinum L.) by immunofluorescence and ELISA technique: Competitive ability for nodule formation between rhizobium strains

Arsac, J. F.; Cleyet-Marel, Jean-Claude

doi:10.1007/bf02374334

Cited by 19 publications

(8 citation statements)

References 11 publications

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“…In a study in France, 100% of the nodules formed were derived from the introduced strain at one field site with a low pH, whereas only 11 % of the investigated nodules were derived from that strain in another soil type, when initially inoculated with the same inoculant level, 5 x lo7 bacteria per seed (Arsac & Cleyet-Marel, 1986). More knowledge of the underlying reasons for the success or failure of the inoculants in different environments is essential.…”

Section: R E S U L T Smentioning

confidence: 99%

Competition in Soil between Effective and Ineffective Strains of Rhizobium leguminosarum biovar trifolii in the Nodulation of Red Clover, Trifolium pratense L., Studied with ELISA

Márrtensson¹,

Gustafsson²,

Ljunggren³

1987

Microbiology

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The ability of two strains of Rhizobium leguminosarurn biovar trifolii (formerly R. trifolii) to compete for nodule sites in soil with indigenous R. 1. biovar trifolii was studied using ELISA. In pot experiments with soil containing effective indigenous strains, one of the inoculated strains, 285, was able to form nodules on red clover (Trifolium pratense L.), not only on the tap root but also on later-formed roots. Under field conditions, also in a soil containing effective indigenous R. 1. biovar trifolii strains, both inoculated strains were detectable in minor proportions in the nodules of the established red clover. The inoculated strains survived in the soil during the field season. As the indigenous strains were effective, no increase in dry matter production was obtained in this experiment. However, in pot experiments in a soil with ineffective indigenous red clover bacteria, the other inoculated strain, 7612, known to be an effective nitrogen-fixing strain, was less successful and was only detectable incidentally. Although the nodule occupancy of the successful competitor strain, 285, only ranged between 7% and 27% on the upper tap root and was merely occasional on the other parts of the root in the 'ineffective' soil, increases in dry matter production were obtained, indicating that the nodules on a specific plant have varying significance for the apparent nitrogen fixation.

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Section: R E S U L T Smentioning

confidence: 99%

Competition in Soil between Effective and Ineffective Strains of Rhizobium leguminosarum biovar trifolii in the Nodulation of Red Clover, Trifolium pratense L., Studied with ELISA

Márrtensson¹,

Gustafsson²,

Ljunggren³

1987

Microbiology

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“…It is possible, however, to select rhizobial strains for competitiveness (Amarger & Lobreau, 1982), and there are indications that selected, highly effective, and competitive strains can be used to increase N2 fixation and yields where native rhizobial populations are low or ineffective (Arsac & Cleyet-Marel, 1986 ;Beck, 1990Beck, , 1992 . Successful nodulation by inoculant strains is often site-specific depending on soil factors and the composition of the indigenous rhizobial population .…”

Section: Controlled Nodulationmentioning

confidence: 99%

Screening techniques and improved biological nitrogen fixation in cool season food legumes

et al. 1993

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Dinitrogen fixation and legume productivity are greatly influenced through the interactions of legume host, Rhizobium, and the above-and below-ground environment . The benefits of improving legume N2 fixation include reduced reliance on soil N, leading to more sustainable agricultural systems and reduced requirements for fertilizer N, enhanced residual benefits to subsequent crops, and increased legume crop yields . Most of the gains in N2 fixation to date have been derived from management of legume cropping systems and through inoculation of legume seed with competitive and symbiotically effective rhizobia . Further gains are possible by developing plant cultivars with tolerance to soil abiotic factors, increased plant yield, and a broader and more effective matching of plant host and rhizobia . Techniques for screening material for superior N2 fixation and examples of programs to increase fixed N, with attention to the major abiotic stresses, are discussed .

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“…Segments were carefully removed from the medium surface, placed in sterile water and crushed. P. brassicacearum cells were specifically counted by indirect immunofluorescence test on glass slides using a polyclonal antiserum produced with whole cells of P. brassicacearum (Arsac & Cleyet-Marel, 1986;Schmidt et al, 1968). the antiserum specificity was ensured by testing all the strains used for competition experiments at different serum and strain dilutions.…”

Section: F Root Colonizationmentioning

confidence: 99%

Bacteria from the rhizosphere and roots ofBrassica napusinfluence its root growth promotion byPhyllobacterium brassicacearum

Larcher¹,

Rapior²,

Cleyet-Marel³

2008

Acta Botanica Gallica

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Abstract.-Phyllobacterium brassicacearum is a plant growth promoting bacterium (PGPB) that stimulates Brassica napus root morphogenesis in absence of bacterial competition. We evaluated in which extent indigenous bacteria associated with B. napus roots and rhizosphere may interfere with the plant growth promoting ability of P. brassicacearum. Thus, bacteria from B. napus roots and rhizosphere were isolated and co-inoculated with the PGPB at different proportions. The root growth promoting ability of P. brassicacearum was impaired only when it was co-inoculated in low proportion together with the indigenous bacteria. This was not linked to a decrease in its population size on roots. Hence, this study highlights that the efficacy of a PGPB is not primarily dependent on the extent of its root colonization but also depends on its initial size compared to the indigenous bacteria. Key words : Brassica napus -Phyllobacterium brassicacearum -plant growth promoting bacteria (PGPB).Résumé.-Phyllobacterium brassicacearum est une bactérie favorisant la croissance des plantes (BFCP) qui stimule la morphogenèse racinaire chez Brassica napus en absence de compétition bactérienne. Nous avons évalué l'impact des bactéries indigènes associées aux racines et à la rhizosphère de B. napus sur la capacité de P. brassicacearum à promouvoir la croissance des plantes. Pour cela, des bactéries associées aux racines et à la rhizosphère de B. napus ont été isolées et co-inoculées avec la BFCP à différentes proportions. La capacité de P. brassicacearum à favoriser la croissance racinaire a été altérée seulement lorsqu'il a été inoculé en proportion minoritaire avec les bactéries indigènes. Cela n'était pas lié à une diminution de sa population sur les racines. Ainsi, cette étude illustre que l'efficacité d'une PGPB ne repose pas seulement sur son potentiel de colonisation racinaire, mais dépend aussi de sa représentativité par rapport aux bactéries indigènes.Mots clés : Brassica napus -Phyllobacterium brassicacearum -bactéries favorisant la croissance des plantes (BFCP).

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Serological and ecological studies ofRhizobium spp. (Cicer arietinum L.) by immunofluorescence and ELISA technique: Competitive ability for nodule formation between rhizobium strains

Cited by 19 publications

References 11 publications

Competition in Soil between Effective and Ineffective Strains of Rhizobium leguminosarum biovar trifolii in the Nodulation of Red Clover, Trifolium pratense L., Studied with ELISA

Competition in Soil between Effective and Ineffective Strains of Rhizobium leguminosarum biovar trifolii in the Nodulation of Red Clover, Trifolium pratense L., Studied with ELISA

Screening techniques and improved biological nitrogen fixation in cool season food legumes

Bacteria from the rhizosphere and roots ofBrassica napusinfluence its root growth promotion byPhyllobacterium brassicacearum

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