Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies

Abstract

A panel of monoclonal antibodies to herpes simplex virus glycoproteins was used for serological analysis of 130 strains. Based on specific immunological determinants, strains of each serotype clustered into subgroups. Monoclonal antibodies were suitable reagents for serotyping and have potential application to epidemiology of herpes simplex virus infections.

In Vitro Selection of Variants of Herpes Simplex Virus Type 1 Which Differ in Cytopathic Changes

“…The gene for glycoprotein C (gC) of HSV-1 (24) is one of the dispensable genes for in vitro growth. Although the coincidence of gC-negative and syncytial phenotypes is often observed during virus propagation in cultured cells (9,20,25), the possibility of gC as a fusion inhibitor in a direct manner was ruled out by marker rescue experiments (13). Certain mutations in the UL27 (glycoprotein B) and UL53 (glycoprotein K) genes were reported to confer a syncytial phenotype (5,10).…”

Glycoprotein gB of herpes simplex virus expresses type‐common and type‐specific antigenic determinants in vivo

“…The numerous in vitro properties of the mouse monoclonal antibodies used in this investigation have been described in previous publications [ Pereira et al, 1982a;Pellet et al, 1985b;Chapsal and Pereira, 1988;Kousoulas et al, 1988, 19891. Relevant < 0.0001 H368 7/10 (70) 9/12 (75) H120 9/10 (90) 8/10 (80) H157 9/10 (90) 10110 (100) ties.…”

“…These glycoproteins appear on the surface of virus-infected cells, where they stimulate the production of and react with various components of immune surveillance [Norrild, 19801. Because of the potential clinical significance of these virus-induced antigens during primary and recurrent herpesvirus disease, much effort has been directed toward their precise immunological characterization. Of particular interest is glycoprotein gB, which has been shown to be essential for HSV-1 infectivity [DeLuca et al, 1982;Little et al, 1981;Manservigi et al, 1977;Sarmiento et al, 1979) and a target for antibody-mediated virus neutralization IEberle and Courtney, 1980;Pereira et al, 1982a I. Additional clinical evidence suggests that gly-1990 WILEY-LISS, INC. coprotein gB is a major immunogen during infection that consistently produces the strongest antibody response in human sera when compared with other virusspecific antigens [Eberle and Mou, 1983 I.…”

Identification and typing of herpes simplex virus types 1 and 2 by monoclonal antibodies, sensitivity to the drug (E)‐5‐(2‐bromovinyl)‐2′‐deoxyuridine, and restriction endonuclease analysis of viral DNA

“…Thus these reports have, for the most part, had to use at least two or more antibodies for both HSV-1 and HSV-2. The added complication of the host cell's glycosylation machinery has led us to seek other markers besides those for the major glycoproteins [Pereira et al, 1981[Pereira et al, , 1982Goldstein et al, 1983;Balachandran et al, 1982a;Peterson et al, 1983;Nilheden et al, 19831. While still not characterized, the late emergence of the antigens after infection, their occurrence on the unenveloped purified nucleocapsid, and the predominant speckled nuclear immunofluorescent patterns argue strongly against either antibody's being one of the major glycoproteins even though the tentative molecular weight is consistent with gC (HSV-1) or gA/B [Balachandran et al, 1982al. Which late HSV protein has such specificity is an open question.…”

“…While several of these reported monoclonal antibodies show some degree of specificity, a single, clearly type 2specific antibody with the desired degree of reactivity and potency for diagnostic purposes does not seem to be available. A panel of antibodies was required in order to detect and identify HSV-1 and HSV-2 [Pereira et al, 1982;Peterson et al, 1983;Nilheden et al, 19831. Furthermore, while the comparison of monoclonal antibody typings with some other techniques is reported, no detailed reports are yet available as to the utilization of monoclonal antibodies to type for drug sensitivity except for a brief report on penile HSV isolates [Docherty et al, 19841. Reported here are the results of studies demonstrating the immunological specificities of two HSV monoclonal antibodies and their successful use in a simple, highly accurate typing of HSV.…”