Serological analysis of feline calicivirus isolates from the United States and United Kingdom

Lauritzen, Alice; Jarrett, Oswald; Sabara, Marta

doi:10.1016/s0378-1135(96)01252-7

Cited by 66 publications

(48 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although the neutralization profile of fresh FCV isolates in 1970s to 1980s when the vaccine was not yet introduced is uncertain, the present results suggest a vaccine adopting a novel antigenic FCV such as 255 strain may give animals more efficient immune protection than the existing F9 type vaccine for instance. In all cases, the epidemiological feature of URI revealed here is similar to those previously described in the Western countries [2,3,9,13,15,20], especially in Britain where the majority of the vaccines incorporate F9 or F9-like strain of FCV have been used [2,3,9,13]. It has been possibly concluded based on these observations that a change in the antigenicity of FCV over the past two decades resulted from either a natural selection or the widespread use of vaccines derived predominantly from a single strain, thus leading to low efficacy of the habitual vaccine [1,4,27].…”

supporting

confidence: 87%

Recent Epidemiological Status of Feline Upper Respiratory Infections in Japan.

Mochizuki

Kawakami

Hashimoto

et al. 2000

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Epidemiology of upper respiratory infections of cats was studied. Nasal, ocular, and oral swabs collected from 111 cats presented at animal hospitals during the past 2.5 years were examined. Twenty-four (21.6%) and 4 (3.6%) cats were diagnosed as feline calicivirus (FCV) infection and feline viral rhinotracheitis, respectively, indicating FCV is more prevalent than feline herpesvirus-1, which revealed a considerable shift from data obtained in 1970s. Cat sera immunized by using vaccines containing either FCV F9 or 255 strains neutralized 42.9% and 66.7% of the FCV isolates, respectively. Chlamydia psittaci, examined by a PCR assay amplifying the ompA gene, was found in 26.9% of 26 diseased cats that typically showed conjunctivitis and rhinitis.KEY WORDS: Chlamydia psittaci, feline calicivirus, feline herpesvirus.

show abstract

supporting

confidence: 87%

Recent Epidemiological Status of Feline Upper Respiratory Infections in Japan.

Mochizuki

Kawakami

Hashimoto

et al. 2000

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…Firstly, it will be important to increase the cross-reactivity of vaccines to maximise the chances of cross-protection, minimise the number of vaccine breakdowns, and minimise the possibilities of evolving vaccine resistant strains [56]. This approach will hopefully help with the control of both typical and more virulent forms of disease.…”

Section: Vaccinesmentioning

confidence: 99%

Feline calicivirus

et al. 2007

View full text Add to dashboard Cite

-Feline calicivirus (FCV) is an important and highly prevalent pathogen of cats. It belongs to the family Caliciviridae which includes other significant pathogens of man and animals. As an RNA virus, high polymerase error rates convey upon FCV a high genome plasticity, and allow the virus to respond rapidly to environmental selection pressures. This makes the virus very adaptable and has important implications for clinical disease and its control. Being genetically diverse, FCV is associated with a range of clinical syndromes from inapparent infections to relatively mild oral and upper respiratory tract disease with or without acute lameness. More recently, highly virulent forms of the virus have emerged associated with a systemic infection that is frequently fatal. A proportion of FCV infected cats that recover from acute disease, remain persistently infected. In such cats, virus evolution is believed to help the virus to evade the host immune response. Such longterm carriers may only represent a minority of the feline population but are likely to be crucial to the epidemiology of the virus. Vaccination against FCV has been available for many years and has effectively reduced the incidence of clinical disease. However, the vaccines do not prevent infection and vaccinated cats can still become persistently infected. In addition, FCV strain variability means that not all strains are protected against equally. Much progress has been made in understanding the biology and pathogenesis of this important feline virus. Challenges for the future will necessarily focus on how to control the variability of this virus particularly in relation to emerging virulent strains and vaccination.

show abstract

“…In the 1990s, however, some studies have shown that the rate of FCV isolation from oropharyngeal swabs of cats with upper respiratory disease is higher than that of feline herpes virus (FHV) [1,5]. It has also been reported that some FCV isolates, which are more resistant to FCV-F9 antibody, are increasing, suggesting the need for re-examination of the efficacy of the existing vaccines [1,6,7]. The most desirable method of evaluating the efficacy of the commercially available vaccines for FCV isolates is to challenge the cats immunized by injection of the vaccines to each isolate.…”

mentioning

confidence: 99%

Neutralizing Feature of Commercially Available Feline Calicivirus (FCV) Vaccine Immune Sera against FCV Field Isolates

Hohdatsu

Sato

Tajima

et al. 1999

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Four types of commercially available feline calicivirus (FCV) vaccine were compared in terms of their efficacy on the basis of the ability of the sera of specific-pathogen-free cats immunized by two injections of each type of vaccine to neutralize FCV field isolates. Each vaccine immune serum neutralized relatively well strains F4, F9, and 255, which were FCV laboratory strains. As to 36 strains of field isolates, however, vaccines A, B, C, and D immune sera did not neutralize 18-20 of the strains (50.0%-55.6%), 19-22 of the strains (52.8%-61.1%), 22-25 of the strains (61.1%-69.4%), and 8-16 of the strains (22.2%-44.4%), respectively. These results indicate that there is much difference in neutralizing antigenicity between the existing vaccine strains and the FCV strains that are prevalent in Japan, suggesting the need for improvement of FCV vaccines.-KEY WORDS: feline calicivirus, neutralizing antigenicity, vaccine immune serum.J. Vet. Med. Sci. 61(3): 299-301, 1999 The FCV used were laboratory strains F4, F9, and 255 and 36 strains of field isolates prepared in Japan in 1991 [9]. Of these 36 strains of field isolates, 23 isolates, 7 isolates, 2 isolates, and each of the remaining 4 isolates were obtained from the oral or nasal swabs of the cats suspected of having viral respiratory diseases, which had been maintained in Tokyo, and Tottori, Yamagata, Ehime, Okayama, Kanagawa, and Saitama Prefectures, respectively. All of the field isolates were purified by three rounds of plaque cloning and propagated in Crandell feline kidney (CrFK) cells.The immune sera were prepared from eight 12-monthold SPF cats immunized by two injections, with an interval of 3 weeks, of each type of feline triple combined vaccine. Serum samples collected 3 weeks after the second injection were used as the immune sera.Serial two-fold dilutions of the test sera were mixed with an equal volume of an FCV strain suspension containing approximately 200 TCID 50 and the mixtures were incubated at 37°C for 60 min. Each mixture was then inoculated into the CrFK cell cultures in flat-bottomed microplates, and incubated in an atmosphere of 5% CO 2 in air at 37°C for 4 days. Each serum dilution was plated in duplicate. The antibody titer was expressed as the reciprocal of the highest dilution of serum that completely inhibited a viral cytopathic effect. Table 1 shows the neutralizing antibody titers of FCV vaccine immune sera against three FCV laboratory strains and 36 strains of FCV field isolates. Each immune serum neutralized three laboratory strains well, with the exception of the reactivity of the vaccine C immune serum against strain 255. The F9 strain particularly was neutralized well with every immune serum. Against the 36 field isolates, however, the immune sera showed various neutralizing antibody titers, and there were eight isolates (91-4, 91-16, 91-19, 91-28, 91-29, 91-34, 91-36, and 91-38) which were not neutralized by any immune serum. Table 2 shows the Feline calicivirus (FCV) is an important cause of acute oral and resp...

show abstract

Serological analysis of feline calicivirus isolates from the United States and United Kingdom

Cited by 66 publications

References 6 publications

Recent Epidemiological Status of Feline Upper Respiratory Infections in Japan.

Recent Epidemiological Status of Feline Upper Respiratory Infections in Japan.

Feline calicivirus

Neutralizing Feature of Commercially Available Feline Calicivirus (FCV) Vaccine Immune Sera against FCV Field Isolates

Contact Info

Product

Resources

About