Serologic Evidence of Influenza A and B Viruses in South American Fur Seals (Arctocephalus australis)

Blanc, Andrea; Ruchansky, Dora; Clara, Mario; Achaval, Federico; Bas, Alfredo Le; Arbiza, Juan

doi:10.7589/0090-3558-45.2.519

Cited by 47 publications

(40 citation statements)

References 16 publications

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“…Influenza A viruses (IAVs) are distributed globally and can infect a wide range of host species, including humans , birds (Chen & Holmes, 2010), pigs , horses , dogs , cats (Ali et al, 2011) and seals (Blanc et al, 2009). Wild waterfowl are considered the natural IAV reservoir (Taubenberger & Kash, 2010) and a genetically distinct lineage of viruses has also been identified in bats (Tong et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Genome plasticity of triple-reassortant H1N1 influenza A virus during infection of vaccinated pigs

Díaz

Enomoto

Romagosa

et al. 2015

Journal of General Virology

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To gain insight into the evolution of influenza A viruses (IAVs) during infection of vaccinated pigs, we experimentally infected a 3-week-old naive pig with a triple-reassortant H1N1 IAV and placed the seeder pig in direct contact with a group of age-matched vaccinated pigs (n510). We indexed the genetic diversity and evolution of the virus at an intra-host level by deep sequencing the entire genome directly from nasal swabs collected at two separate samplings during infection. We obtained 13 IAV metagenomes from 13 samples, which included the virus inoculum and two samples from each of the six pigs that tested positive for IAV during the study. The infection produced a population of heterogeneous alleles (sequence variants) that was dynamic over time. Overall, 794 polymorphisms were identified amongst all samples, which yielded 327 alleles, 214 of which were unique sequences. A total of 43 distinct haemagglutinin proteins were translated, two of which were observed in multiple pigs, whereas the neuraminidase (NA) was conserved and only one dominant NA was found throughout the study. The genetic diversity of IAVs changed dynamically within and between pigs. However, most of the substitutions observed in the internal gene segments were synonymous. Our results demonstrated remarkable IAV diversity, and the complex, rapid and dynamic evolution of IAV during infection of vaccinated pigs that can only be appreciated with repeated sampling of individual animals and deep sequence analysis.

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Section: Introductionmentioning

confidence: 99%

Genome plasticity of triple-reassortant H1N1 influenza A virus during infection of vaccinated pigs

Díaz

Enomoto

Romagosa

et al. 2015

Journal of General Virology

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“…By Hemagglutination Inhibition Assay (HAI) it was found that all the positive samples reacted with A/New Caledonia/20/99(H1N1) antigen reaching HAI titer of 320 but none of the sampled serum reacted with A/Panamá/2007/99(H3N2) antigen. For the first time, the presence of influenza A in A. australis was confirmed (Blanc et al, 2009) (Fig. 6).…”

Section: Evidence Of Influenza Virus Infections In Arctocephalus Austmentioning

confidence: 66%

“…Indirect evidence of influenza A viruses has been reported in 27% of the South American fur seals sampled in Uruguay (Blanc et al, 2009). By Hemagglutination Inhibition Assay (HAI) it was found that all the positive samples reacted with A/New Caledonia/20/99(H1N1) antigen reaching HAI titer of 320 but none of the sampled serum reacted with A/Panamá/2007/99(H3N2) antigen.…”

Section: Evidence Of Influenza Virus Infections In Arctocephalus Austmentioning

confidence: 94%

“…Serologic evidence of influenza B virus has been reported from South American Uruguayan fur seals A. australis (Blanc et al, 2009). Thirty of the 37 serum samples assayed by HAI reacted against one of the three antigens used: 25/37 (68%) reacted against B/Beijing/184/93-like viruses, 20/37 (54%) reacted against B/Hong Kong/330/01, and 24/37 (65%) reacted against B/Sichuan/379/99.…”

Section: Evidence Of Influenza Virus Infections In Arctocephalus Austmentioning

confidence: 99%

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Uruguayan Pinnipeds (Arctocephalus australis and Otaria flavescens): Evidence of Influenza Virus and Mycobacterium pinnipedii Infections

Arbiza¹,

Blanc²,

Castro-Ramos³

et al. 2012

New Approaches to the Study of Marine Mammals

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“…Furthermore, isolation of M. pinnipedii in pups without gross lesions indicates that the bacterium is acquired in early stages of their life. Serological studies have reported exposure to influenza A and B viruses in SAFS pups in Uruguay [36], but it is unknown if it could act as a primary or predisposing cause of respiratory infections. In the New Zealand sea lions, pulmonary and systemic infections by Klebsiella pneumoniae have contributed to significant pup mortality [21], but it is unknown if this bacteria could be affecting SAFS.…”

Section: Discussionmentioning

confidence: 99%

Pathological and Parasitological Findings in South American Fur Seal Pups (Arctocephalus australis) in Uruguay

2012

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This paper presents the necropsy findings in South American fur seal (Arctocephalus australis) pups from Uruguayan colonies. Animals (n = 105) were aged between 0 and 18 months old. From 0 to 6 months, 69.4% of the pups showed a poor body condition, while 68.8% of animals from 7 to 18 months had fair-to-good body condition. From 6 to 7 months of age, the stomach content included fishes, crustaceans, and foreign bodies. Starvation in the first months of life and traumatic lesions in pups older than 9 months were the most frequent causes of death. Uncinaria spp. was the only parasite found in the small intestine between 0 and 6 month-old pups. Parasites with indirect cycle (Contracaecum spp., Corynosoma sp., Tetrabothriidae) were present from 6 months of age as well as the first report of the nematode Strongyloides spp. in pinnipeds. Orthohalarachne spp. was found in the respiratory tract. Mycobacterium pinnipedii was isolated from 9 animals without gross pathological lesions. Other pathological conditions were found in lesser extent. This information contributes to the main causes of death of A. australis pups at different ages and could be useful to perform further health studies on this wild pinniped species.

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Serologic Evidence of Influenza A and B Viruses in South American Fur Seals (Arctocephalus australis)

Cited by 47 publications

References 16 publications

Genome plasticity of triple-reassortant H1N1 influenza A virus during infection of vaccinated pigs

Genome plasticity of triple-reassortant H1N1 influenza A virus during infection of vaccinated pigs

Uruguayan Pinnipeds (Arctocephalus australis and Otaria flavescens): Evidence of Influenza Virus and Mycobacterium pinnipedii Infections

Pathological and Parasitological Findings in South American Fur Seal Pups (Arctocephalus australis) in Uruguay

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