Serologic evidence of human metapneumovirus circulation in Uruguay

Mirazo, Santiago; Ruchansky, Dora; Blanc, Andrea

doi:10.1590/s0074-02762005000700005

Cited by 18 publications

(14 citation statements)

References 10 publications

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“…A study examining a chimeric virus vaccine based on alphavirus replicon particles encoding the G or F protein of HMPV demonstrated that the virus harboring the F protein of HMPV induced protective immunity against subsequent challenge. The latter observation made for HMPV is in contrast to the case for alphavirus harboring the G protein, which did not appear to be immunogenic and protective (170). A live-attenuated HMPV vaccine candidate was generated by repeated passages of HMPV at low temperature in Vero cells, resulting in the accumulation of mutations in the viral genome (111).…”

Section: Vaccine Developmentmentioning

confidence: 97%

Human Metapneumovirus: Lessons Learned over the First Decade

Schildgen

Hoogen²,

Fouchier³

et al. 2011

Clin Microbiol Rev

181

187

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SUMMARY It has been 10 years since human metapneumovirus (HMPV) was identified as a causative agent of respiratory illness in humans. Since then, numerous studies have contributed to a substantial body of knowledge on many aspects of HMPV. This review summarizes our current knowledge on HMPV, HMPV disease pathogenesis, and disease intervention strategies and identifies a number of areas with key questions to be addressed in the future.

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Section: Vaccine Developmentmentioning

confidence: 97%

Human Metapneumovirus: Lessons Learned over the First Decade

Schildgen

Hoogen²,

Fouchier³

et al. 2011

Clin Microbiol Rev

181

187

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“…Additionally, reverse transcription polymerase chain reaction was used to detect RSV and influenza virus, 18 PIV 1-3, 19 and human metapneumovirus (hMPV), 20 and polymerase chain reaction was used for AdV. 21 RT-PCR for HRV was performed by using a combination of 2 protocols.…”

Section: Underlying Risk Conditionsmentioning

confidence: 99%

Human Rhinovirus and Disease Severity in Children

Costa¹,

Queiróz²,

Silveira

et al. 2014

Pediatrics

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WHAT'S KNOWN ON THIS SUBJECT: Human rhinovirus has been known as the common cold agent. Recently, studies have reported that this virus is responsible for severe infections of the lower respiratory tract in children. Reports of factors that increase disease severity have been contradictory. WHAT THIS STUDY ADDS:This study identifies some of the factors involved in disease severity in HRV infections in children. We expect that children at risk for developing severe disease could be identified sooner and appropriate measures could be taken. abstract OBJECTIVE: To evaluate retrospectively human rhinovirus (HRV) infections in children up to 5 years old and factors involved in disease severity.METHODS: Nasopharyngeal aspirates from 434 children presenting a broad range of respiratory infection symptoms and severity degrees were tested for presence of HRV and 8 other respiratory viruses. Presence of host risk factors was also assessed.RESULTS: HRV was detected in 181 (41.7%) samples, in 107 of them as the only agent and in 74 as coinfections, mostly with respiratory syncytial virus (RSV; 43.2%). Moderate to severe symptoms were observed in 28.9% (31/107) single infections and in 51.3% (38/74) coinfections (P = .004). Multivariate analyses showed association of coinfections with lower respiratory tract symptoms and some parameters of disease severity, such as hospitalization. In coinfections, RSV was the most important virus associated with severe disease. Prematurity, cardiomyopathies, and noninfectious respiratory diseases were comorbidities that also were associated with disease severity (P = .007).CONCLUSIONS: Our study showed that HRV was a common pathogen of respiratory disease in children and was also involved in severe cases, causing symptoms of the lower respiratory tract. Severe disease in HRV infections were caused mainly by presence of RSV in coinfections, prematurity, congenital heart disease, and noninfectious respiratory disease. Pediatrics 2014;133:e312-e321 AUTHORS:

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“…The nucleoprotein (N) gene was chosen for hMPV screening through a reverse transcription-polymerase chain reaction (RT-PCR) assay with specific primers [Mirazo et al, 2005]. cDNA was synthesized in a 20 ml reaction at 428C for 50 min with primer N1: 5 0 -ATGG-GACAAGTGAAAATGTC-3 0 [Cote et al, 2003] and Superscript II 1 Reverse Transcriptase (Invitrogen, Grand Island, NY).…”

Section: Rna Extraction Rt-pcr Assays and Sequencingmentioning

confidence: 99%

“…Later, different studies have also described the presence of hMPV in Peru, Chile, and Brazil [Cuevas et al, 2003;Serafino et al, 2004;Luchsinger et al, 2005;Gray et al, 2006;Carneiro et al, 2009]. The first report on the circulation of hMPV in Uruguay has been a serologic study carried out by Mirazo et al [2005]. A total of 69 serum samples obtained from adults from the National Service of Blood, and from children attending the Paediatric Hospital Pereira Rossell of Montevideo were tested, providing indirect evidence of the circulation of hMPV in Uruguay at least since 1998.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and genetic variability of human metapneumovirus in Uruguay

Pizzorno

Masner

Medici

et al. 2010

Journal of Medical Virology

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Human metapneumovirus (hMPV) has been described as circulating among the Uruguayan population at least since 1998 based on serologic evidence. However, no isolation attempts, molecular detection, or genetic studies have been carried out so far in the country. In the present study, molecular detection of circulating hMPV in children hospitalized with acute respiratory tract infection in Montevideo-Uruguay was carried out by reverse transcription-polymerase chain reaction (RT-PCR) amplification of the hMPV nucleoprotein (N) gene from 217 nasopharyngeal aspirates. Genetic variability analysis of the positive samples was performed by amplification and sequencing of both N and attachment glycoprotein (G) genes. Eighteen of the 217 samples tested positive for hMPV, with tachypnea, chest indrawing, and wheezing being the main clinical symptoms recorded. Phylogenetic analysis of N and G genes showed that Uruguayan samples clustered in genotypes described previously as A2, B1, and B2, with bootstrap values >or=98%. Sublineages A2a and A2b could also be distinguished within the samples that belong to A2. This is the first molecular report on the circulation of hMPV in Uruguay. The pattern of circulation of this virus, analyzed for both N and G genes independently, resembles the complex evolutionary pattern of respiratory syncytial virus (RSV).

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Serologic evidence of human metapneumovirus circulation in Uruguay

Cited by 18 publications

References 10 publications

Human Metapneumovirus: Lessons Learned over the First Decade

Human Metapneumovirus: Lessons Learned over the First Decade

Human Rhinovirus and Disease Severity in Children

Molecular detection and genetic variability of human metapneumovirus in Uruguay

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