Serologic differentiation of viruses responsible for cytomegalic inclusion disease

Weller, Thomas H.; Hanshaw, James B.; Scott, D.

doi:10.1016/0042-6822(60)90156-2

Cited by 162 publications

(47 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Table 3 shows the strikingly higher incidence of antibodies in the boarding school population. (Weller, Hanshaw & Scott, 1960). The London strains were 'Aravi' isolated from a 6-monthold child with a malignant giant haemangioma, 'Sh' isolated from an apparently healthy 7-day-old baby who was found to have an enlarged liver and spleen and a reduced platelet count, and ' 138' and 'Rawles' isolated respectively from a fatal case of neonatal cytomegalic inclusion disease and from a 4-year-old boy with symptomless hepatomegaly and abnormal liver function tests (Stern et al 1963; Stern & Tucker, unpublished).…”

Section: Resultsmentioning

confidence: 99%

The incidence of infection with cytomegalovirus in a normal population: A serological study in Greater London

Stern

Elek

1965

J. Hyg.

176

View full text Add to dashboard Cite

A serological study of cytomegalovirus infection in London shows it to be prevalent. Under 5 years of age only 4% of children have antibodies, but this increases to 15% by 10 years of age and 21% by 15 years. The maximum incidence of 54% is reached by 25–35 years, and this is maintained in the older age groups of the population. In two mixed day schools the incidence of infection was significantly lower than in a boarding school, suggesting that close prolonged contact is required for spread of infection.Different strains of cytomegalovirus possess common group-specific complement-fixing antigens.It is suggested that clinical disease caused by cytomegaloviruses is more frequent and more varied than previously believed, although diagnosis may be difficult without virological help.

show abstract

Section: Resultsmentioning

confidence: 99%

The incidence of infection with cytomegalovirus in a normal population: A serological study in Greater London

Stern

Elek

1965

J. Hyg.

176

View full text Add to dashboard Cite

show abstract

“…In addition, it has been associated with glioblastoma and other cancers (2), cardiovascular disease (3), and immune sensescence (4). Cytomegalovirus is named for the morphological changes -enlarged cells with nuclear and cytoplasmic inclusions-observed in cytomegalic inclusion disease (5). Striking morphological changes have been documented after infection of cultured fibroblasts with HCMV, including cell rounding, enlarged nuclei with a distorted kidneylike shape, and production of a membranous structure adjacent to the concave surface of the nucleus known as the assembly compartment (6)(7)(8).…”

mentioning

confidence: 99%

Human cytomegalovirus pUL37x1-induced calcium flux activates PKCα, inducing altered cell shape and accumulation of cytoplasmic vesicles

Sharon-Friling

Shenk

2014

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The human cytomegalovirus immediate-early protein pUL37x1 induces the release of Ca 2+ stores from the endoplasmic reticulum into the cytosol. This release causes reorganization of the cellular actin cytoskeleton with concomitant cell rounding. Here we demonstrate that pUL37x1 activates Ca 2+ -dependent protein kinase Cα (PKCα). Both PKCα and Rho-associated protein kinases are required for actin reorganization and cell rounding; however, only PKCα is required for the efficient production of virus progeny, arguing that HCMV depends on the kinase for a second function. PKCα activation is also needed for the production of large (1-5 μm) cytoplasmic vesicles late after infection. The production of these vesicles is blocked by inhibition of fatty acid or phosphatidylinositol-3-phosphate biosynthesis, and the failure to produce vesicles is correlated with substantially reduced production of enveloped virus capsids. These results connect earlier work identifying a requirement for lipid synthesis with specific morphological changes, and support the argument that the PKCα-induced large vesicles are either required for the efficient production of mature virus particles or serve as a marker for the process.capsid envelopment | virion assembly | virus-induced membranes

show abstract

“…There are likely to be a range of antigenic differences between strains (Weller, Hanshaw & Scott, 1960;Dreesman & Benyesh-Melnick, 1967;Krech & Jung, 1969) but it does appear from the preceding observations that reactivation could be a major factor in the series described.…”

Section: Discussionmentioning

confidence: 99%

Cytomegalovirus antibody production in renal transplant patients

Nagington

1971

Epidemiol. Infect.

View full text Add to dashboard Cite

SUMMARYSera were examined from 50 patients on the renal transplant unit, Cambridge, for antibody against cytomegalovirus by complement fixation and by immunofluorescence for IgG and IgM antibodies.The incidence of antibody on admission was 84 % with a possible further 8 % so that nearly all had been infected at some time by CMV. 43 (86 %) patients showed evidence of active infection after admission, 39 by serology and four only from the examination of post-mortem material.Twenty-one patients produced IgM antibody and production was prolonged for years in patients that survived. Antibody production was related both to transplantation and admission to hospital.The evidence indicated that primary CMV infections were rare, that IgM antibody production was the result of active infection and that this could be attributed to reactivation without the need to invoke re-infection as the source although this type of patient is both susceptible and exposed to re-infection.

show abstract

Serologic differentiation of viruses responsible for cytomegalic inclusion disease

Cited by 162 publications

References 3 publications

The incidence of infection with cytomegalovirus in a normal population: A serological study in Greater London

The incidence of infection with cytomegalovirus in a normal population: A serological study in Greater London

Human cytomegalovirus pUL37x1-induced calcium flux activates PKCα, inducing altered cell shape and accumulation of cytoplasmic vesicles

Cytomegalovirus antibody production in renal transplant patients

Contact Info

Product

Resources

About