Serologic Diagnosis of Bone and Joint Tuberculosis by an Enzyme-Linked Immunosorbent Assay

Stroebel, Adam B.; Daniel, Thomas M.; Lau, James H. K.; Leong, Jcy; Richardson, Harold

doi:10.1093/infdis/146.2.280

Cited by 49 publications

(18 citation statements)

References 14 publications

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“…Recent studies have shown encouraging results with enzyme-linked immunosorbent assays (ELISA) for the serodiagnosis of tuberculosis (Nassau, Parsons and Johnson, 1976;Tandon et al, 1980;Stroebel et al, 1982;Benjamin and Daniel, 1982;Kalish et al, 1983;. There have been some differences in the ELISA methodology, but the major differences have been in the antigens used to detect antibody in patient and control sera.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Mycobacterial Antigens in an Enzyme-Linked Immunosorbent Assay (ELISA) for the Serodiagnosis of Tuberculosis

Benjamin

Debanne

et al. 1984

Journal of Medical Microbiology

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SUMMARY. Five mycobacterial antigens were compared in an enzymelinked immunosorbent assay (ELISA) for the serodiagnosis of tuberculosis. The antigens studied were an unheated sterile culture filtrate of Mycobacterium tuberculosis, tuberculin purified protein derivative (PPD) from M . tuberculosis (PPDJ, purified cytoplasmic protein antigens 5 and 6 from M. tuberculosis, and a PPD prepared from M . kansasi (PPDk). Multivariate analysis of variance showed that geometric mean titres obtained with each of the antigens in ELISA were significantly different in tuberculosis patients and in control groups. The covariation of the ELISA results with the five antigens was highly interdependent. Analysis of receiver operating characteristics revealed that the most accurate test was obtained with antigen 5. M . tuberculosis PPD, M . tuberculosis antigen 6, and M. tuberculosis culture filtrate were, in descending order, less accurate.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Mycobacterial Antigens in an Enzyme-Linked Immunosorbent Assay (ELISA) for the Serodiagnosis of Tuberculosis

Benjamin

Debanne

et al. 1984

Journal of Medical Microbiology

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“…This antigen reacts with sera from most patients having multibacillary or advanced pulmonary tuberculosis, but it is poorly recognized in sera from patients having low bacillary counts in sputum and from asymptomatic infected persons (for examples, see references 7, 9, 15, 32, and 43). Likewise, antibodies against the Ag85 complex are associated with smear-positive pulmonary disease and correlate with the extent of disease as determined by radiography, while they are absent in patients with past tuberculosis infections, asymptomatic infections, or recent exposures to M. tuberculosis (5,11,34,35). The high-level transcription of esat-6 in both multiplying and nonreplicating tubercle bacilli also fits well with the notion that ESAT-6 induces cellular immune responses in both active disease and in latent infection (3,13,27,39).…”

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confidence: 99%

Effect of Growth State on Transcription Levels of Genes Encoding Major Secreted Antigens of Mycobacterium tuberculosis in the Mouse Lung

Shi¹,

North

Gennaro³

2004

Infect Immun

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Arrest of the multiplication of Mycobacterium tuberculosis caused by expression of adaptive immunity in mouse lung was accompanied by a 10-to 20-fold decrease in levels of mRNAs encoding the secreted Ag85 complex and 38-kDa lipoprotein. esat-6 mRNA levels were high throughout infection. The data imply that multiplying and nonreplicating tubercle bacilli have different antigen compositions.The seminal finding that live Mycobacterium tuberculosis elicit protective immunity more effectively than dead bacilli (6, 24) prompted extensive investigation of proteins that tubercle bacilli secrete as potential targets of protective immune responses. Among the best-characterized secreted proteins are the low-molecular-weight ESAT-6 protein (33), the antigen 85 complex (Ag85A, Ag85B, and Ag85C) (42), encoded by the fbpA, fbpB, and fbpC genes, and a 38-kDa glycolipoprotein (17), the product of pstS1. These antigens induce strong immune responses to infection with M. tuberculosis or Mycobacterium bovis, and they elicit protective immunity in animal models of tuberculosis (for a review, see references 1, 4, and 16). Consequently, experimental vaccines based on ESAT-6 and/or the Ag85 complex have been scheduled for clinical trials (18, 23), ESAT-6 has been proposed for immunodiagnosis of tuberculosis (3,10,13,20,26), and the 38-kDa protein is included in all serodiagnostic assays for active tuberculosis developed to date. To extend the usefulness of these proteins as new drug targets, the structures of two members of the Ag85 complex (2, 29) and the 38-kDa antigen (40) have been solved by X-ray crystallography and nuclear magnetic resonance has been used to obtain the structure of the cotranscribed ESAT-6/CFP-10 complex (28). Next we need to understand the production of these target antigens at various stages of human infection.Classic biochemical studies (30) and recent gene manipulation experiments (21) showed that M. tuberculosis changes its metabolic state during infection by utilizing alternative metabolic pathways. We have shown (31) that adaptation of M. tuberculosis to the expression of adaptive immunity in the lungs of infected mice involves changes in the pathogen's transcription program characteristic of the state of nonreplicating persistence (41). In the present work, we characterize a correlation between the growth state of M. tuberculosis and the production of major secreted antigens by measuring levels of M. tuberculosis transcripts encoding the Ag85 complex, ESAT-6, and the 38-kDa lipoprotein during M. tuberculosis infection of the mouse lung. The data support the view that antigen composition differs between multiplying and nonreplicating tubercle bacilli.Infection of C57BL/6 mice and isogenic, gamma interferon knockout (IFN-␥ Ϫ/Ϫ ) mice with ϳ2 ϫ 10 2 CFU of M. tuberculosis strain H 37 Rv (Trudeau Mycobacterial Collection strain no. 102) cultivated to mid-log-growth phase in Proskauer and Beck medium containing 0.01% Tween 80 was carried out as previously described (14, 31). At selected times, lungs were harve...

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“…It is possible that our results could have been affected by the inclusion of a large number of patients with extrapulmonary and smear-nega tive disease. Although the reliability of the ELISA test has not been widely assessed in patients with extrapulmonary tuberculosis, Stroebel et al [22] and Chawla et al [23] have reported encouraging results.…”

Section: Discussionmentioning

confidence: 99%

Value of ELISA Using A60 Antigen in the Serodiagnosis of Tuberculosis

Caminero

Castro²,

Carrillo³

et al. 1994

Respiration

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This study was set in a general, university hospital in the Canary Islands, with the objective to evaluate an enzyme-linked immunosorbent assay (ELISA) using A60 in the serodiagnosis of tuberculosis. IgG antibody to A60 was determined in 205 patients with active disease [149 culture-positive patients with pulmonary tuberculosis (positive sputum smear 94, negative sputum smear 55) and 56 patients with extrapulmonary tuberculosis], 20 patients with inactive disease, 22 patients with lepromatous leprosy, and 51 controls. The mean levels of anti-A60 antibodies were significanctly higher in patients with active disease as compared with controls or patients with inactive disease. Differences were also found between tuberculous patients with pulmonary and extrapulmonary disease. In patients with pulmonary disease, significant differences were detected between smear-positive and smear-negative patients. The overall sensitivity of the test (cutoff 240 ELISA units) was 52.2%. The highest sensitivity was found among smear-positive patients with pulmonary tuberculosis (67%) and the lowest among those with extrapulmonary tuberculosis (32.1%). We conclude that ELISA for the measurement of IgG antibody to Mycobacterium tuberculosis antigen A60 could be of interest, specially in smear-negative cases and extrapulmonary tuberculosis.

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Serologic Diagnosis of Bone and Joint Tuberculosis by an Enzyme-Linked Immunosorbent Assay

Cited by 49 publications

References 14 publications

Evaluation of Mycobacterial Antigens in an Enzyme-Linked Immunosorbent Assay (ELISA) for the Serodiagnosis of Tuberculosis

Evaluation of Mycobacterial Antigens in an Enzyme-Linked Immunosorbent Assay (ELISA) for the Serodiagnosis of Tuberculosis

Effect of Growth State on Transcription Levels of Genes Encoding Major Secreted Antigens of Mycobacterium tuberculosis in the Mouse Lung

Value of ELISA Using A60 Antigen in the Serodiagnosis of Tuberculosis

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