Serologic Assays Specific to Immunoglobulin M Antibodies against Hepatitis E Virus: Pangenotypic Evaluation of Performances

Abstract

Six immunoassays for detecting immunoglobulin M antibodies to hepatitis E virus were evaluated. Serum samples representing acute infection by each of the 4 viral genotypes as well as nonacute hepatitis E virus infection constituted the test panels. Diagnostic sensitivities and specificities as well as interassay agreement varied widely. Analytical sensitivity limits also were determined and were found to be particularly disparate.

Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?

“…Diagnostic performances and results differ in specificity and sensitivity of used commercially available assays. Serological findings depend on the principle of enzyme-immunoassays (EIA; indirect or µ-capture method) and the used antigen selections and conformations [5,14,[18][19][20][21][22][23][24]. Although HEV is antigenically consistent and only one serotype has been identified, recombinant antigens used for antibody testing are not optimized.…”

Acute Hepatitis E Virus infection in two geographical regions of Nigeria

“…Additionally, studies have indicated that commercially Journal of Pathogens 3 available ELISA kits for anti-HEV antibodies differ dramatically in their sensitivity and specificity [34][35][36]. In our study, we used an ELISA kit that has been confirmed to have the highest diagnostic sensitivity and specificity when compared to many others by several studies [35,37].…”

Hepatitis-E-Virus

“…-4-Antigene eingesetzt waren, zeigten Unterschiede in der Reaktivität mit diesen Antigenen [159]. Ebenso wurden in Antikör-pernachweissystemen Unterschiede in der Reaktivität von IgM-und IgG-Antikör-pern gegen verschiedene HEV-Antigene festgestellt [160,161].…”