Serologic Assay for Diagnosis of Celiac Disease Based on a Patient-Derived Monoclonal Antigliadin Antibody

Steinsbø, Øyvind; Dørum, Siri; Lundin, Knut E.A.; Sollid, Ludvig M.

doi:10.1053/j.gastro.2015.07.008

Cited by 6 publications

(4 citation statements)

References 38 publications

(55 reference statements)

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“…Recently, AlphaLISA has rapidly been developed for a wide range of clinical applications because of its high sensitivity, high throughput, and simple operation procedure. Some clinical diagnostic assays have appeared, including hepatitis B surface antigen, HE4, allergic celiac disease, and preeclampsia detection kits, among others . Our competitive AlphaLISA showed the optimal performance for serum stathmin‐1 detection.…”

Section: Discussionmentioning

confidence: 90%

A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma

et al. 2018

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Stathmin‐1 is a microtubule depolymerization protein that regulates cell division, growth, migration, and invasion. Overexpression of stathmin‐1 has been observed to be associated with metastasis, poor prognosis, and chemoresistance in various human cancers. Our previous studies found that serum stathmin‐1 was significantly elevated in patients with esophageal squamous cell carcinoma (ESCC) by ELISAs. Here, we constructed high‐affinity monoclonal antibodies and then developed a competitive AlphaLISA for rapid, accurate quantitation of stathmin‐1 in serum. Compared to ELISA, our homogeneous AlphaLISA showed better sensitivity and accuracy, a lower limit of detection, and a wider linear range. The measurements of nearly 1000 clinical samples showed that serum stathmin‐1 level increased dramatically in patients with squamous cell carcinoma (SCC), especially in ESCC, with a sensitivity and a specificity of 81% and 94%, respectively. Even for early stage ESCC, stathmin‐1 achieved an area under the receiver operating characteristic curve (AUC) of 0.88. Meanwhile, raised concentrations of stathmin‐1 were associated with lymph node metastasis and advanced cancer stage. Notably, various types of SCC showed significantly higher AUCs in serum stathmin‐1 detection compared to adenocarcinoma. Furthermore, we confirmed that stathmin‐1 was enriched in the oncogenic exosomes, which can explain the reason why it enters into the blood to serve as a tumor surrogate. In conclusion, this large‐scale and systematic study of serum stathmin‐1 measured by our newly established AlphaLISA showed that stathmin‐1 is a very promising diagnostic and predictive marker for SCC in the clinic, especially for ESCC.

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Section: Discussionmentioning

confidence: 90%

A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma

et al. 2018

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“…12 "Normal" Mucosae Harbor Subtle Pathologic Processes Laboratory-based advances in celiac disease diagnosis have been COMMENTARIES facilitated through IgA antiendomysial and antitransglutaminase (TG2) antibodies assayed serologically, immunohistologically, or from cultured biopsy supernatants; and IEL characterized by immunohistology or flow cytometry. Assays for highly discriminatory gluten subfractions 13 or deamidated gliadins 14 might, ultimately, undermine traditional histopathology.…”

Section: Historical Overviewmentioning

confidence: 99%

What Is A Normal Intestinal Mucosa?

Marsh

Rostami

2016

Gastroenterology

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“…Definiert wird die Zöliakie als eine entzündliche Enteropathie, ausgelöst durch den Verzehr von Gluten bei genetisch prädisponierten Personen mit potenziell systemischen Komplikationen. [20].…”

Section: Merkeunclassified

Zöliakie und Weizensensitivität im Wandel – ein Update

Hübener¹,

Manthey²

2020

Aktuel Ernahrungsmed

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Coeliac disease is a disease in flux ? both the presentation of the disease and its therapy are changing. Furthermore, in addition to classical coeliac disease, which is characterized by a duodenal villi atrophy, there are also other causes of intolerance to wheat and other cereals. These have become the subject of not only scientific research in recent years, but also intensive public debate. Thus, further diseases can be distinguished from conventional coeliac disease defined by a T-cell-mediated immune response to gliadin peptides. This article provides an update on the diagnosis and the state of development of new therapies.

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Serologic Assay for Diagnosis of Celiac Disease Based on a Patient-Derived Monoclonal Antigliadin Antibody

Abstract: We developed an antigliadin inhibition assay that identifies patients with celiac disease with high levels of specificity and sensitivity. It may prove useful as an adjunct to the current assay for anti-TG2 IgA.

Cited by 6 publications

References 38 publications

A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma

A novel rapid quantitative method reveals stathmin‐1 as a promising marker for esophageal squamous cell carcinoma

What Is A Normal Intestinal Mucosa?

Zöliakie und Weizensensitivität im Wandel – ein Update

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