Serogroups, K1 antigen, and antimicrobial resistance patterns of Aeromonas spp. strains isolated from different sources in Mexico

Garibay, R.I Arteaga; Aguilera‐Arreola, Ma. Guadalupe; Navarro, Armando; Cerezo, Silvia Giono; Mendoza, Miroslava Sánchez; López, José Molina; Campos, Carlos Eslava; Cravioto, Alejandro; Castro-Escarpulli, Graciela

doi:10.1590/s0074-02762006000200007

Cited by 8 publications

(3 citation statements)

References 17 publications

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“…; Arteaga Garibay et al . ), it constituted 12.2% of β‐hemolytic isolates from foods with higher frequency in chicken. Emergence of virulent A. caviae strains has been reported in recent past, particularly from Indian subcontinent (Abbott et al .…”

Section: Resultsmentioning

confidence: 99%

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Occurrence and Virulence Characteristics of Aeromonas Species in Meat, Milk and Fish in India

Manna

Maurye

Dutta

et al. 2013

Journal of Food Safety

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Aeromonas-induced gastroenteritis is common in India. The incidence of Aeromonas in meat, milk and fish in Kolkata, India and their enterotoxic potentiality have been evaluated here. Aeromonas contamination was detected in all or most of the meat and fish with average load of 1.1 × 10 2 , 3.5 × 10 3 , 1.1 × 10 3 , 2.1 × 10 3 and 1.6 × 10 4 cfu/g of red meats, chicken, Indian major carps, tilapia and shrimp, respectively; the levels were at least one order higher on fish skin and meat surface. Aeromonas was also detected in milk and ready-to-eat foods. About 59% of the isolates were β-hemolytic, and 66-92% of them produced phospholipase, caseinase and enterotoxicity. Fish and shrimp isolates produced significantly higher levels of gelatinase (102.73-120.6 U/mL), hemolysin (causing >80% erythrocyte lysis) and enteric fluid accumulation (0.86-0.89 mL/cm ileum length) compared with those from meat and milk indicating their serious pathogenic potentiality. Phenotypically, 43.2, 12.2 and 12.2% of β-hemolytic strains were identified as recognized human pathogenic species, viz. Aeromonas hydrophila, Aeromonas caviae and Aeromonas veronii bv. sobria, respectively, which also expressed higher levels of virulence factors than other Aeromonas species detected. The ubiquitous presence of enterotoxigenic Aeromonas in different raw as well as ready-to-eat foods poses serious threat to human health. PRACTICAL APPLICATIONSUbiquitous presence of enterotoxigenic Aeromonas hydrophila, Aeromonas caviae and Aeromonas veronii bv. sobria in raw and ready-to-eat meat, milk and fish poses a serious human health risk in Indian subcontinent. Heavy bacterial load, combined with higher expression of virulence factors by the isolates, makes fish and shellfish as the most potential sources of Aeromonas infection. Different Aeromonas spp. differed in quantitative expression of different virulence factors. Qualitative and quantitative expression studies showed low positive correlations among gelatinase, hemolysin and ileal enterotoxicity, suggesting requirement of studying multiple virulence factors for identifying the virulent strains. A higher distribution of the bacterium on meat or fish surface and in unhygienically processed or stored food items indicates that adoption of standard practices of cleaning and sanitation would substantially improve safety of food products.infections in human (Janda and Abbott 2010). Aeromonasinduced gastroenteritis is characterized by acute profuse watery diarrhea in all age groups, but more frequently in young children (Albert et al.

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Section: Resultsmentioning

confidence: 99%

“…; Alavandi and Ananthan ; Arteaga Garibay et al . ; Janda and Abbott ; Khajanchi et al . ; Ottaviani et al .…”

Section: Resultsmentioning

confidence: 99%

Occurrence and Virulence Characteristics of Aeromonas Species in Meat, Milk and Fish in India

Manna

Maurye

Dutta

et al. 2013

Journal of Food Safety

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“…In turn, the variable chemical structure of the O-polysaccharide portion determines the immunospecificity of individual bacterial strains and has become the basis for classification thereof to appropriate O serogroups. Serological typing has been used in epidemiological studies to define the routes of transmission or relationships with pathogenicity [49]. It is known that the specific O serogroups can be associated with certain clinical syndromes.…”

Section: Discussionmentioning

confidence: 99%

A Unique Sugar l-Perosamine (4-Amino-4,6-dideoxy-l-mannose) Is a Compound Building Two O-Chain Polysaccharides in the Lipopolysaccharide of Aeromonas hydrophila Strain JCM 3968, Serogroup O6

et al. 2019

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Lipopolysaccharide (LPS) is the major glycolipid and virulence factor of Gram-negative bacteria, including Aeromonas spp. The O-specific polysaccharide (O-PS, O-chain, O-antigen), i.e., the surface-exposed part of LPS, which is a hetero- or homopolysaccharide, determines the serospecificity of bacterial strains. Here, chemical analyses, mass spectrometry, and 1H and 13C NMR spectroscopy techniques were employed to study the O-PS of Aeromonas hydrophila strain JCM 3968, serogroup O6. MALDI-TOF mass spectrometry revealed that the LPS of A. hydrophila JCM 3968 has a hexaacylated lipid A with conserved architecture of the backbone and a core oligosaccharide composed of Hep6Hex1HexN1HexNAc1Kdo1P1. To liberate the O-antigen, LPS was subjected to mild acid hydrolysis followed by gel-permeation-chromatography and revealed two O-polysaccharides that were found to contain a unique sugar 4-amino-4,6-dideoxy-l-mannose (N-acetyl-l-perosamine, l-Rhap4NAc), which may further determine the specificity of the serogroup. The first O-polysaccharide (O-PS1) was built up of trisaccharide repeating units composed of one α-d-GalpNAc and two α-l-Rhap4NAc residues, whereas the other one, O-PS2, is an α1→2 linked homopolymer of l-Rhap4NAc. The following structures of the O-polysaccharides were established: O-PS1 →3)-α-l-Rhap4NAc-(1→4)-α-d-GalpNAc-(1→3)-α-l-Rhap4NAc-(1→ O-PS2 →2)-α-l-Rhap4NAc-(1→ The present paper is the first work that reveals the occurrence of perosamine in the l-configuration as a component of bacterial O-chain polysaccharides.

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Deciphering genome-wide molecular pathways for exogenous Aeromonas hydrophila infection in wide-bodied sand loach (Sinibotia reevesae)

Lv,

Li,

Huang

et al. 2024

Aquaculture Reports

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Serogroups, K1 antigen, and antimicrobial resistance patterns of Aeromonas spp. strains isolated from different sources in Mexico

Abstract: A total of 221 strains of Aeromonas species isolated in Mexico from clinical (161), environmental (40), and food (20)

Cited by 8 publications

References 17 publications

Occurrence and Virulence Characteristics of Aeromonas Species in Meat, Milk and Fish in India

Occurrence and Virulence Characteristics of Aeromonas Species in Meat, Milk and Fish in India

A Unique Sugar l-Perosamine (4-Amino-4,6-dideoxy-l-mannose) Is a Compound Building Two O-Chain Polysaccharides in the Lipopolysaccharide of Aeromonas hydrophila Strain JCM 3968, Serogroup O6

Deciphering genome-wide molecular pathways for exogenous Aeromonas hydrophila infection in wide-bodied sand loach (Sinibotia reevesae)

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