Serogroups and antibiotics susceptibility pattern of avian pathogenic Escherichia coli strains responsible for colibacillosis in broiler breeding farms in the east of Algeria

Abstract

In East Algeria, 100 Escherichia coli strains were isolated from livers and spleens of 120 broiler carcasses that presented colibacillosis lesions at autopsy. Serogrouping of these strains demonstrated that 83% of their serogroups belong to the most pathogenic serogroups of Avian Pathogenic Escherichia coli (APEC): O1 (14%), O2 (53%) and O78 (16%). In vitro susceptibility to antimicrobials of veterinary significance was determined by disc diffusion test. Antibiogram demonstrated a high level of resistance to enrofloxacin (82%), trimethoprim-sulfmethoxazole (82%), ampicillin (89%) and amoxicillin/ Ac clavulanic (90%), nalidixic acid (99%) and tetracycline (100%). There were moderate levels of resistance to neomycin (49%) and nitrofurantoin (53%). There were low levels of resistance to gentamicin (2%), chloramphenicol (13%) and no resistance to colistin sulfate (0%). All strains were multi-drug resistant and more than half (58%) of the isolates were resistant to seven antibiotics at least. Thus, thirty one antibiotic resistant patterns of E. coli strains were detected, of which 11 were present significantly. Co-resistance was found in eight of the eleven most common multidrug resistant patterns, 62% of the strains expressed this co-resistance. E. coli strains isolated expressed resistances to molecules that are routinely used in the field. It is clear that these are less effective against colibacillosis. It is more than ever necessary to perform antibiotic susceptibility testing prior to treatment in order to prescribe the molecule of choice, and set up a monitoring program in Algeria to monitor antimicrobial resistance in pathogenic bacteria that could be potentially transmitted to humans from animal food.

Isolation and Molecular Identification of Avian Pathogenic Escherichia coli in Broiler Chickens Suffering from Colibacillosis in some Governorates in Egypt

“…In this study, isolates of E. coli O27 strains showed high level of resistance against amoxicillin, ampicillin, oxytetracycline, trimetoprim-sulfamethoxazole, lincomycin, streptomycin, norfloxacin, cephradine, and spiramycin but high sensitive to difloxacin. Similar result weas recorded by Messaï et al [37] who studied the antimicrobial resistance of APEC strains responsible for colibacillosis in broiler breeding farms in eastern Algeria and Sarker et al [38] reported resistance to ampicillin and tetracycline among multi drug resistance (MDR) E. coli isolated from broiler chickens. Moawad et al [39] observed high resistant E. coli isolates from raw chicken meat with the different antibiotics (ampicillin (71.4%), tetracycline (80.9%), streptomycin (61.9%), trimethoprim/ sulphamethoxazole (61.9%), and cefotaxime 33.3%).…”

Antimicrobial resistance pattern of avian pathogenic Escherichia coli with detection of extended-spectrum β-lactamase-producing isolates in broilers in east Algeria

“…Resistance to SXT was lower than in previous results in Algeria and worldwide. Messai et al [5] and Ibrahim et al [3] found high resistance to SXT at 82% and 95.50%, respectively. Resistance to CN, C, and NIT was higher than in Benameur et al [27], Halfaoui et al [19], Boutaiba Benklaouz et al [12], and Kazibwe et al [24].…”

“…Colibacillosis remains one of the most common, economic, and cost-effective bacterial diseases in the poultry industry, particularly among broilers globally and in east Algeria [ 5 ].…”

“…Messai et al . [ 5 ] and Ibrahim et al . [ 3 ] found high resistance to SXT at 82% and 95.50%, respectively.…”

“…Colibacillosis is considered an economically devastating disease in Algeria and worldwide, leading to huge losses translated by high mortality, decreased performance, condemnation of carcasses at slaughter, and increased preventive and curative costs [4][5][6]. Accordingly, antibiotic therapy plays a key role in colibacillosis infection treatment.…”