Seroepidemiological survey for antibodies to arboviruses in Greece

Ántoniadis, Antonis; Alexiou-Daniel, S.; Malissiovas, Nikolaos; Doutsos, J.; Polyzoni, Thalia; LeDue, J. W.; Peters, C. J.; Saviolakis, George A.

doi:10.1007/978-3-7091-9091-3_31

Cited by 31 publications

(30 citation statements)

References 7 publications

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“…Antibodies against WNV have been present in domestic animals, as well as in ∼1% of the humans in Greece since the 1960s [4][5][6][7][8]. Hemagglutination-inhibiting (HAI) antibodies against WNV were identified in sera obtained from sheep, goats, bovine, horses, mules, as well as in one rabbit, one hare and one pig.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a West Nile virus surveillance and early warning system in Greece, based on domestic pigeons

Chaintoutis¹,

Dovas²,

Papanastassopoulou³

et al. 2014

Comparative Immunology, Microbiology and Infectious Diseases

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a b s t r a c tIn the summer of 2010 an epidemic of West Nile virus (WNV) occurred in Central Macedonia, Greece, with 197 human neuroinvasive disease (WNND) cases. In the following years the virus spread to new areas, with a total of 76 WNND cases in 2011, and 109 WNND cases in 2012 (14 and 12 WNND cases, respectively, in Central Macedonia). We established a surveillance system based on serological testing of domestic pigeons, using cELISA confirmed by serum neutralization test. In Central Macedonia, pigeon seroprevalence was 54% (95% CI: 49-59%) and 31% (95% CI: 24-37%) at the end of the 2010 and 2011 epidemic seasons, respectively. One serum was positive for neutralizing antibodies directed against Usutu virus. Pigeon WNV seroprevalence and incidence rates of human WNND after the 2010 epidemic were positively correlated ( = 0.94, at the regional unit level), while in 2011 the correlation ( = 0.56) was not statistically significant, possibly due to small number of human WNND cases recorded. To evaluate the efficacy of the system at alerting upon WNV enzootic circulation before the onset of human cases, we tested 270 pigeons in 2011 and 240 pigeons in 2012. In Central Macedonia, the first seroconversions in pigeons were recorded 44 and 47 days, respectively, before the first human WNND cases. Pigeon surveillance was used successfully for identification of areas with WNV enzootic transmission and for early warning. Timely diffusion of information to health authorities facilitated the implementation of preparedness plans to protect public health.

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Section: Introductionmentioning

confidence: 99%

Evaluation of a West Nile virus surveillance and early warning system in Greece, based on domestic pigeons

Chaintoutis¹,

Dovas²,

Papanastassopoulou³

et al. 2014

Comparative Immunology, Microbiology and Infectious Diseases

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“…Three serotypes, Sicilian (SFS), Naples (SFN), and Toscana (TOS), were associated with clinical infections in humans. SFS and SFN infections are endemic in several Mediterranean countries, the Middle East, and Central Asia [2][3][4][5][6][7][8][9], and these viruses are transmitted by bites of the insect vector Phiebotomus papatasi (sandfly) [10,11]. In the early 1980s, TOS was isolated in Italy from Phlebotomus perniciosus [5].…”

Section: Introductionmentioning

confidence: 99%

Serosurvey and laboratory diagnosis of imported sandfly fever virus, serotype Toscana, infection in Germany

et al. 1995

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SUMMARYOf eight acute infections in German tourists caused by sandfly fever virus, serotype Toscana (TOS), and diagnosed clinically and serologically, seven were acquired during visits to Tuscany, Italy, and one to Coimbra, Portugal. An indirect immunofluorescence assay (IFA) using infected cells, and a newly developed enzyme-immunoassay (EIA) using crude virus antigen prepared from infected Vero-E6 cells was used to detect anti-TOS IgM and IgG. In a seroepidemiological survey of 859 health care workers and medical students, anti-TOS IgG was detected in 1-0% by IFA, and in 0 7 % by EIA. In 2034 German patients hospitalized for various diseases, 1 6 % were positive for anti-TOS IgG by IFA, and 0-8 % by EIA. Anti-TOS IgG was detected in 43 samples of commercial immunoglobulins at titres of 10-1000 by EIA. Although the seroprevalence of antibodies to TOS is low in Germany, TOS infection should be considered in patients returning from endemic areas who complain of fever, and headaches, and have symptoms of meningitis.

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“…The disease has not yet been reported in Greece, but fatal cases have been reported from neighbouring countries (Albania, Bulgaria and the former Yugoslavia). However, the virus has been isolated from ticks in Greece (Papadopoulos & Koptopoulos, 1980) and serological surveys have shown human antibodies to CCHF virus, especially in woodcutters and farmers, who are likely to be exposed to the tick vector (Antoniadis et al, 1990). Diagnosis currently relies on immunofluorescence of virus-infected cells or isolation of virus in suckling mouse brain.…”

Section: Introductionmentioning

confidence: 99%

Detection of human antibodies to Crimean-Congo haemorrhagic fever virus using expressed viral nucleocapsid protein

Marriott¹,

Polyzoni

Antoniadis

et al. 1994

Journal of General Virology

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Diagnosis of Crimean-Congo haemorrhagic fever (CCHF) virus infections is hampered by the problems of handling this human pathogen, which requires the highest levels of biological containment. Recombinant antigens were examined for their potential as nonhazardous diagnostic reagents. The nucleocapsid (N) gene of the Greek AP92 isolate of CCHF virus was sequenced from cloned PCR products and the open reading frame was identified by homology to the N protein of a Chinese isolate of CCHF virus. The N protein was expressed to high levels in a baculovirus expression system. Three N protein-derived peptides were expressed in Eseherichia coli as fusions with glutathione S-transferase and the antigenicities of these proteins and the baculovirus-expressed protein were tested by ELISA. When tested with laboratory animal sera representing all seven serogroups of nairoviruses, the only reactive sera were those raised to CCHF virus (Greek, Nigerian and Chinese isolates) and, more weakly, Hazara virus. When tested with a panel of known positive and negative human sera, the baculovirus-expressed N protein, and the peptide derived from the central region of the N protein, proved to be the best for identifying CCHF virus-specific IgG.

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Seroepidemiological survey for antibodies to arboviruses in Greece

Cited by 31 publications

References 7 publications

Evaluation of a West Nile virus surveillance and early warning system in Greece, based on domestic pigeons

Evaluation of a West Nile virus surveillance and early warning system in Greece, based on domestic pigeons

Serosurvey and laboratory diagnosis of imported sandfly fever virus, serotype Toscana, infection in Germany

Detection of human antibodies to Crimean-Congo haemorrhagic fever virus using expressed viral nucleocapsid protein

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