Serodiagnostic Potential of Culture Filtrate Antigens of
            <i>Mycobacterium tuberculosis</i>

Samanich, Karen M.; Keen, Marc A.; Vissa, Varalakshmi; Harder, Jeffery D.; Spencer, John S.; Belisle, John T.; Zolla‐Pazner, Susan; Laal, Suman

doi:10.1128/cdli.7.4.662-668.2000

Cited by 65 publications

(90 citation statements)

References 22 publications

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“…Differences noted in our study could be due to the paediatric population having different disease pathology. More numbers of smear negative patients were found to be reactive to these antigens than the earlier report [25]. Raja et al have reported sensitivity of 65.4% using 30 kDa antigen; however, sensitivity was increased by combining IgG, IgM and IgA response in childhood tuberculosis [13].…”

Section: Discussioncontrasting

confidence: 43%

Diagnostic Potential of Ag85C in Comparison to Various Secretory Antigens for Childhood Tuberculosis

et al. 2008

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Childhood tuberculosis is difficult to diagnose. A rapid, simple and relatively inexpensive diagnostic test will be crucial to future control efforts. Therefore, efficacy and diagnostic potential of different secretory antigens of Mycobacterium tuberculosis (CFP‐10, Ag85complex, Ag85 A, B, C) and their combinations along with ESAT‐6 in the detection of antibody profiles of childhood tuberculosis cases were evaluated using ELISA technique and reactivity was compared with the gold standards (smear, culture and IS6110 targeted PCR). In the present study, 88 fresh, untreated childhood tuberculosis (TB) cases, 17 children undergoing anti‐tubercular therapy, 17 children having disease other than TB and 25 healthy children were included. ROC curve analysis was used to calculate the sensitivity and specificity of each antigen for antibody detection. Ag85C was found to be showing highest sensitivity of 89.77% and specificity of 92% among all the antigens used (P < 0.0001). Positivity with antigen was 95% in smear and culture negative patients. Antibody reactivity was noted in 92.62% of patients who were positive for IS6110 by PCR. Cocktail of all the antigens showed 67.1% sensitivity and 80% of specificity. Sensitivity of 29.55%, 57.95%, 64.77% and specificity of 80%, 72%, 64% was observed using CFP‐10, Ag85complex and Ag85B. Low reactivity of 31.82% in patients and least specificity of 24% was noted with Ag85A. Our finding demonstrates the potential of Ag85C in the detection of antibody in childhood TB cases and this antigen showed good concordance with PCR positivity.

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Section: Discussioncontrasting

confidence: 43%

Diagnostic Potential of Ag85C in Comparison to Various Secretory Antigens for Childhood Tuberculosis

et al. 2008

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“…Algunos autores afirman que el paciente responde a determinados antígenos según el tiempo de evolución de la enfermedad, por ejemplo, los antígenos 38 kd y 30 kd se han detectado casi exclusivamente en estadios avanzados de la enfermedad (17).…”

Section: Discussionunclassified

“…Se han estudiado diversos antígenos de manera individual y obtenidos de forma recombinante entre los cuales tenemos: la proteína de 38 kd (15)(16)(17)(18) (24,25).…”

Section: Development Of a Multiantigenic Serological Test For Tubercuunclassified

Utilidad de una prueba serológica multiantigénica en el diagnóstico de tuberculosis.

Castro¹,

Porras²,

Guerrero³

et al. 2005

biomedica

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“…The immunogenicity of these antigens also differs: only a small number of the antigens released in the culture media (25%) induced the synthesis of specific antibodies. (Samanich et al, 2000). The variability of released antigens under different conditions and their immunogenicity hinders an accurate selection and represents a serious obstacle in the immunodiagnostic development of TB.…”

Section: Mycobacterial Antigens (General Data Classification Role)mentioning

confidence: 99%

Immunologic Diagnosis of Neurotuberculosis

Alexandra¹,

Dorina²,

Gabriela³

et al. 2012

Understanding Tuberculosis - Global Experiences and Innovative Approaches to the Diagnosis

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Serodiagnostic Potential of Culture Filtrate Antigens of Mycobacterium tuberculosis

Cited by 65 publications

References 22 publications

Diagnostic Potential of Ag85C in Comparison to Various Secretory Antigens for Childhood Tuberculosis

Diagnostic Potential of Ag85C in Comparison to Various Secretory Antigens for Childhood Tuberculosis

Utilidad de una prueba serológica multiantigénica en el diagnóstico de tuberculosis.

Immunologic Diagnosis of Neurotuberculosis

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