Serodiagnosis Using Microagglutination Assay during the Food-Poisoning Outbreak in Japan Caused by Consumption of Raw Beef Contaminated with Enterohemorrhagic Escherichia coli O111 and O157

Isobe, Junko; Shima, Tomoko; Kanatani, Jun-ichi; Kimata, Keiko; Shimizu, Miwako; Kobayashi, Naoto; Tanaka, Tomoko; Iyoda, Sunao; Ohnishi, Makoto; Sata, Tetsutaro; Watahiki, Masanori

doi:10.1128/jcm.03469-13

Cited by 15 publications

(16 citation statements)

References 26 publications

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“…IMS was performed using magnetic beads functionalized with specific antibodies against the serogroup previously identified by the glyco-iELISAs. In five of these samples (see Table S2, patients 17,18,19,33, and 36), we were able to confirm the diagnosis by isolation of an STEC strain of the expected serogroup.…”

Section: Resultsmentioning

confidence: 79%

“…Currently, serological tests for the diagnosis of STEC-associated HUS use whole bacteria, bacterial extracts containing high concentrations of LPS, or purified LPS as antigens (10,11,(15)(16)(17). Consequently, these tests may lack serospecificity due to the presence of cross-reactive antibodies against common epitopes present in the core polysaccharide and lipid A of LPS shared by different STEC serotypes.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of antibodies directed against the LPS in the serum of patients with HUS was initially reported by Chart, Scotland, and Rowe in 1989 (16). Since then, antibodies against this antigen have been measured using different methods, such as immunoblotting (16), enzyme-linked immunosorbent assay (ELISA) (11,15), indirect hemagglutination assay (IHA) (10), and a microagglutination (MA) assay (17). All these assays use the whole bacteria, bacterial extracts containing high concentrations of lipopolysaccharide (LPS), or purified LPS as antigens.…”

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confidence: 99%

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Serogroup-Specific Bacterial Engineered Glycoproteins as Novel Antigenic Targets for Diagnosis of Shiga Toxin-Producing-Escherichia coli-Associated Hemolytic-Uremic Syndrome

et al. 2015

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Human infection with Shiga toxin-producing Escherichia coli (STEC) is a major cause of postdiarrheal hemolytic-uremic syndrome (HUS), a life-threatening condition characterized by hemolytic anemia, thrombocytopenia, and acute renal failure. E. coli O157:H7 is the dominant STEC serotype associated with HUS worldwide, although non-O157 STEC serogroups can cause a similar disease. The detection of anti-O157 E. coli lipopolysaccharide (LPS) antibodies in combination with stool culture and detection of free fecal Shiga toxin considerably improves the diagnosis of STEC infections. In the present study, we exploited a bacterial glycoengineering technology to develop recombinant glycoproteins consisting of the O157, O145, or O121 polysaccharide attached to a carrier protein as serogroup-specific antigens for the serological diagnosis of STEC-associated HUS. Our results demonstrate that using these antigens in indirect ELISAs (glyco-iELISAs), it is possible to clearly discriminate between STEC O157-, O145-, and O121-infected patients and healthy children, as well as to confirm the diagnosis in HUS patients for whom the classical diagnostic procedures failed. Interestingly, a specific IgM response was detected in almost all the analyzed samples, indicating that it is possible to detect the infection in the early stages of the disease. Additionally, in all the culture-positive HUS patients, the serotype identified by glyco-iELISAs was in accordance with the serotype of the isolated strain, indicating that these antigens are valuable not only for diagnosing HUS caused by the O157, O145, and O121 serogroups but also for serotyping and guiding the subsequent steps to confirm diagnosis. Shiga toxin-producing Escherichia coli (STEC) is an important food-borne pathogen associated with sporadic cases and outbreaks of diarrhea, bloody diarrhea (BD), and hemolytic-uremic syndrome (HUS), a life-threatening condition characterized by microangiopathic hemolytic anemia, thrombocytopenia, and acute renal failure (1). Postdiarrheal HUS is caused by particular serotypes of STEC or, in regions, such as South Asia, by Shigella dysenteriae serotype 1 (2). STEC strains are characterized by the production of Shiga toxin 1 (Stx1) and/or Shiga toxin 2 (Stx2), and the production of these toxins is central in the pathogenesis of BD and HUS (3). E. coli O157:H7 is the dominant STEC serotype associated with sporadic cases and outbreaks of BD and HUS in different parts of the world (4); however, a subset of non-O157:H7 STEC serotypes (O26:H11, O103:H2, O111:NM, O121:H19, O145:NM, and O45:H2, among others) can cause a similar disease (5, 6).STEC-associated HUS is the most common cause of acute renal failure in children worldwide and constitutes 90% of all HUS cases in this population. Five to 10 percent of children with STEC infection develop HUS, for which the only treatment is supportive care. The incidence rate of postdiarrheal HUS varies according to country, and Argentina shows the highest HUS incidence worldwide, with 12 to 14 cases per 100,000 ...

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Section: Resultsmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Serogroup-Specific Bacterial Engineered Glycoproteins as Novel Antigenic Targets for Diagnosis of Shiga Toxin-Producing-Escherichia coli-Associated Hemolytic-Uremic Syndrome

et al. 2015

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“…In the serum samples of 10 patients of the 16 group 4 HUS cases, the anti-O111 antibody was detectable in all samples, whereas the antibody to O157 was only detectable in two samples. These data indicate that EHEC O111 isolates caused most infections, but these patients were no longer shedding EHEC (32). An interesting feature of this outbreak was that stx-negative E. coli O111 strains were isolated from patient stool specimens.…”

Section: Isolation Of Ehec From Patientsmentioning

confidence: 81%

“…The remaining 16 HUS cases were EHEC negative, even after extensive efforts to isolate EHEC, with up to 800 E. coli colonies examined after O111 enrichment using immunomagnetic beads. Since serologically positive results for the E. coli O111 antigen were found from all but one of the EHEC-negative HUS cases (32), EHEC O111 may be the primary cause of the severe HUS complications. An stx-negative E. coli O111 strain was isolated from most EHEC-positive HUS cases (13 of 18 HUS cases) and from a few EHEC-negative HUS cases (3 of 16 HUS cases).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Enterohemorrhagic Escherichia coli O111 and O157 Strains Isolated from Outbreak Patients in Japan

et al. 2014

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In April and May 2011, there was a serious food-poisoning outbreak in Japan caused by enterohemorrhagic Escherichia coli (EHEC) strains O111:H8 and O157:H7 from raw beef dishes at branches of a barbecue restaurant. This outbreak involved 181 infected patients, including 34 hemolytic-uremic syndrome (HUS) cases (19%). Among the 34 HUS patients, 21 developed acute encephalopathy (AE) and 5 died. Patient stool specimens yielded E. coli O111 and O157 strains. We also detected both EHEC O111 stx 2 and stx-negative E. coli O111 strains in a stock of meat block from the restaurant. Pulsed-field gel electrophoresis (PFGE) and multilocus variable-number tandem-repeat analysis (MLVA) showed that the stx-negative E. coli O111 isolates were closely related to EHEC O111 stx 2 isolates. Although the EHEC O157 strains had diverse stx gene profiles (stx 1 , stx 2 , and stx 1 stx 2 ), the PFGE and MLVA analyses indicated that these isolates originated from a single clone. Deletion of the Stx2-converting prophage from the EHEC O111 stx 2 isolates was frequently observed during in vitro growth, suggesting that strain conversion from an EHEC O111 stx 2 to an stx-negative strain may have occurred during infection.

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Larva migrans syndrome caused by Toxocara and Ascaris roundworm infections in Japanese patients

Yoshida

Hombu

Wang

et al. 2016

Eur J Clin Microbiol Infect Dis

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Larva migrans syndrome (LMS) caused by Toxocara and Ascaris roundworms is generally believed to be more common in children, while a report from Japan suggests that it is more common in adults. We conducted a large-scale retrospective study to confirm these findings and to clarify what caused the difference between Japan and other countries, to reveal overlooked aspects of this disease. The clinical information of 911 cases which we diagnosed as Toxocara or Ascaris LMS during 2001 and 2015 was analysed. Information used included age, sex, address (city or county), chief complaint, present history, dietary history, overseas travelling history, medical imaging findings and laboratory data (white blood cell count, peripheral blood eosinophil number and total IgE). The sex ratio of the disease was 2.37 (male/female = 641/270). The number of patients not younger than 20 years old were 97.8 and 95.1 % among males and females, respectively. Major disease types were visceral, ocular, neural and asymptomatic. The visceral type was more prevalent in older patients, while younger patients were more vulnerable to ocular symptoms. More than two-thirds of the patients whose dietary habits were recorded had a history of ingesting raw or undercooked animal meat. LMS caused by Toxocara or Ascaris is primarily a disease of adult males in Japan, who probably acquired infections by eating raw or undercooked animal meat/liver. Healthcare specialists should draw public attention to the risk of raw or undercooked animal meat in Europe as well.

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Serodiagnosis Using Microagglutination Assay during the Food-Poisoning Outbreak in Japan Caused by Consumption of Raw Beef Contaminated with Enterohemorrhagic Escherichia coli O111 and O157

Cited by 15 publications

References 26 publications

Serogroup-Specific Bacterial Engineered Glycoproteins as Novel Antigenic Targets for Diagnosis of Shiga Toxin-Producing-Escherichia coli-Associated Hemolytic-Uremic Syndrome

Serogroup-Specific Bacterial Engineered Glycoproteins as Novel Antigenic Targets for Diagnosis of Shiga Toxin-Producing-Escherichia coli-Associated Hemolytic-Uremic Syndrome

Characterization of Enterohemorrhagic Escherichia coli O111 and O157 Strains Isolated from Outbreak Patients in Japan

Larva migrans syndrome caused by Toxocara and Ascaris roundworm infections in Japanese patients

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