Serodiagnosis of tuberculosis by enzyme immunoassay using A60 antigen

Yüce, Ayşe; Yücesoy, Mine; Genç, Şermin; Sayan, Murat; Uçan, Eyüp Sabri

doi:10.1046/j.1198-743x.2001.00263.x

Cited by 10 publications

(3 citation statements)

References 21 publications

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“…Other workers investigated the diagnostic value of the serum IgM levels against A60 antigen in extrapulmonary and smear‐negative pulmonary TB, and the results indicated a sensitivity of 10.5% and a specificity of 99.4% for IgM [17]. A recent study from Turkey, using A60 by ELISA in the sera of active smear‐ and/or culture‐positive lung TB cases, demonstrated that the sensitivity and specificity values were 53.6% and 94.7% for IgG, and 43.8% and 97.4% for IgM [20]. Thus, results from different sources show great variations, depending on the population studied and the site of the disease.…”

Section: Discussionmentioning

confidence: 99%

The value of pleural fluid anti-A60 IgM in BCG-vaccinated tuberculous pleurisy patients

Kunter¹,

Cerrahoğlu²,

İlvan³

et al. 2003

Clinical Microbiology and Infection

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Section: Discussionmentioning

confidence: 99%

The value of pleural fluid anti-A60 IgM in BCG-vaccinated tuberculous pleurisy patients

Kunter¹,

Cerrahoğlu²,

İlvan³

et al. 2003

Clinical Microbiology and Infection

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“…The diagnostic methods presented so far have been direct diagnostic methods, while serology offers an alternative, indirect approach to pathogen detection. Serology, screening for the presence of specific antibodies in the serum of animals, can be a useful indicator of previous exposure to a pathogen, especially for viruses, and this type of screening is used routinely in clinical and veterinary medicine (Palmer‐Densmore, Johnson & Samara 1998; Yüce, Yücesoy, Genç, Sayan & Uçan 2001; Fournier & Raoult 2003).…”

Section: New Molecular Methods With Potential For Use In Aquaculturementioning

confidence: 99%

Development of diagnostics for aquaculture: challenges and opportunities

Adams

Thompson

2011

Aquaculture Research

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The application of biotechnology in aquaculture has enabled the development and improvement of a wide range of immunodiagnostic and molecular technologies, and reagents and commercial kits have become more generally available. Recently, method development has increased exponentially as techniques used for clinical and veterinary medicine are adapted and optimized for use in aquaculture. Careful consideration needs to be given to selecting which rapid diagnostic methods to take forward and apply in aquaculture^pathogen detection methods need to be robust yet sensitive, and in many cases, capable of detecting a high degree of heterogeneity. There are numerous innovative techniques that may ful¢l these criteria and provide valuable diagnostic tools. It is also important, however, that useful diagnostic methods already developed are standardized and fully validated, and that new technologies do not supercede these just because they are novel methods. The cost, speed, speci¢city and sensitivity of assays are all extremely important to end-users. This paper looks at some of the opportunities and challenges for the development of rapid diagnostics for aquaculture.

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“…In a study performed in Ghana [120], using an immunochromatographic serodiagnostic test for simultaneous detection of IgG, IgA and IgM specific for A60, the sensitivity was 80% and the specificity was 98%. In a study conducted in Turkey [121], the sensitivity and specificity of three antibody isotypes, IgG, IgA and IgM, specific for A60 were compared in three patients groups (those with active TB, latent TB and miliary TB). The results showed that the detection of IgA specific for A60 was the most sensitive, with positivity rates of 78.1%, 73.7% and 37.5% for active, miliary and inactive TB cases respectively.…”

Section: Antigen (A60)mentioning

confidence: 99%

Progress in Serodiagnosis of Mycobacterium tuberculosis Infection

et al. 2007

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One‐third of the world population is estimated to have Mycobacterium tuberculosis infection. Accurate and timely identification of infected individuals is critical for treatment and control. The current diagnostic methods lack the desired sensitivity and specificity, require sophisticated equipment and skilled workforce or take weeks to yield results. Diagnosis of extrapulmonary TB, TB‐HIV co‐infection, childhood TB and sputum smear‐negative pulmonary TB pose serious challenges. Interest in developing serodiagnostic methods is increasing because detection of antibody is rapid, simple and relatively inexpensive, and does not require a living cell for detection. Three types of tests, namely screening tests to overcome diagnostic delay, specific tests for diagnosis of extrapulmonary TB and other bacteriologically negative cases, and tests for vaccine‐induced immunity need critical consideration. Several factors must be considered to develop serodiagnostic methods for TB. Antigen recognition by infected individuals is highly heterogeneous due to stage of disease, differences in HLA types, strain of the bacilli, health of the patient and bacillary load. With advances in molecular biological techniques, a number of novel antigens have been identified. Some of these antigens have proven valuable in detecting specific antibodies in some of the most challenging TB patients. The best example is a fusion protein containing several M. tuberculosis proteins (e.g. CFP‐10, MTB8, MTB48, MTB81 and the 38‐kDa protein) which showed encouraging results in detecting antibodies in sera of patients, including TB‐HIV co‐infection. This review presents progress made in the serodiagnosis of TB during the last decade.

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Serodiagnosis of tuberculosis by enzyme immunoassay using A60 antigen

Cited by 10 publications

References 21 publications

The value of pleural fluid anti-A60 IgM in BCG-vaccinated tuberculous pleurisy patients

The value of pleural fluid anti-A60 IgM in BCG-vaccinated tuberculous pleurisy patients

Development of diagnostics for aquaculture: challenges and opportunities

Progress in Serodiagnosis of Mycobacterium tuberculosis Infection

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