Serodiagnosis of Sudanese visceral and mucosal leishmaniasis: comparison of ELISA-immunofluorescence and indirect haemagglutination

Mohammed, El Amin El Roufaie; Wright, Edward; A, Rahman; Kolk, A. H. J.; Laarman, J. J.; Pondman, K. W.

doi:10.1016/0035-9203(86)90033-7

Cited by 31 publications

(5 citation statements)

References 19 publications

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“…These methods tend to be created according to the preferences of each laboratory, and may cause discordant results between different laboratories. In addition, crude antigen preparations made from whole promastigotes or their soluble extracts limit the standardization of testing and the reproducibility of results [26][27][28] . When using ELISA as a serological test, we found significantly conflicting results when different antigen preparations were used.…”

Section: Discussionmentioning

confidence: 99%

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

Lima

Gennari

Soares

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Section: Discussionmentioning

confidence: 99%

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

Lima

Gennari

Soares

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…Because of the retrospective character of this study, duration of fixation is not exactly known in these cases. Other diagnostic approaches like ELISA or IFAT have the disadvantage that cross-reactivity with other protozoal parasites, especially T cruzi, Ehrlichia species, Rickettsia species and Toxoplasma species may lead to false-positive results (Mohammed and others 1986, Harith and others 1987, Da Costa and others 1991, Barbosa-de-Deus and others 2002).…”

Section: Discussionmentioning

confidence: 99%

In situ hybridisation for the detection of Leishmania species in paraffin wax‐embedded canine tissues using a digoxigenin‐labelled oligonucleotide probe

et al. 2011

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The diagnosis of canine leishmaniosis (CanL) is currently predominantly achieved by cytological or histological identification of amastigotes in biopsy samples, demonstration of specific antiLeishmania antibodies and PCR-based approaches. All these methods have the advantage of being sensitive and more or less specific; nevertheless, most of them also have disadvantages. A chromogenic in situ hybridisation (ISH) procedure with a digoxigenin-labelled probe, targeting a fragment of the 5.8S rRNA was developed for the detection of all species of Leishmania parasites in routinely paraffin wax-embedded canine tissues. This method was validated in comparison with traditional techniques (histology, PCR), on various tissues from three dogs with histological changes consistent with a florid leishmaniosis. Amastigote forms of Leishmania gave clear signals and were easily identified using ISH. Various tissues from 10 additional dogs with clinical suspicion or/and a positive serological test but without histological presence of amastigotes did not show any ISH signals. Potential cross-reactivity of the probe was ruled out by negative outcome of the ISH against selected protozoa (including the related Trypanosoma cruzi) and fungi. Thus, ISH proved to be a powerful tool for unambiguous detection of Leishmania parasites in paraffin waxembedded tissues.OF the approximately 20 virulent species of Leishmania, a considerable number has been (Dantas-Torres 2007). Clinical disease in dogs, referred to as canine leishmaniosis (CanL) seems to be caused by only one species, L infantum (syn L chagasi) in many regions worldwide. These protozoal parasites of great medical and veterinary significance are transmitted by the bite of female blood-sucking phlebotomine sand fly vectors of the genera Phlebotomus in the Old World (Desjeux 1996) and Lutzomyia in the New World (Grimaldi and Tesh 1993).Based on serological surveys, it has been estimated that at least 2.5 million dogs are infected in the south-western European countries (Moreno and Alvar 2002). Although most of these dogs are clinically asymptomatic, they constitute a very significant part of the reservoir of L infantum, the aetiological agent of zoonotic visceral leishmaniosis (Dantas-Torres 2007 Frequently, skin biopsies and samples from lymphatic tissue are used for diagnosing CanL. Apart from the microscopic identification of amastigotes, IHC procedures are available. However, commercially available monoclonal antibodies failed to produce specific staining in paraffin wax-embedded tissue and alternatively used canine hyperimmune sera are not readily available for all laboratories and may be cross-reactive with related protozoa (Bourdoiseau and others 1997, Tafuri and others 2004). Thus the major aim of the present study was to fill this diagnostic gap and to establish an in situ hybridisation (ISH) procedure for Leishmania species which facilitates detection of parasites directly within the tissue and which should be specific and sensitive. Material and methods Tissue sam...

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“…Its sensitivity ranges from 90% to 100% and the specificity is about 80% for serum samples (12,17). It is a sensitive technique, nevertheless, with cross reactions possibility, especially between Chagas' disease and American Cutaneous Leishmaniasis (16).…”

Section: The First Report On the Occurrence Of Canine Visceral Leishmmentioning

confidence: 99%

American visceral Leishmaniasis: a case report

Langoni¹,

Lucheis²,

Silva³

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

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Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT) demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of Polymerase Chain Reaction (PCR) utilized the primers LINR4 and LIN19, which amplify 720 base pairs, specific for Leishmania sp. The authors discuss the importance of techniques for a quick and precise diagnosis to this serious zoonosis with great impact in animal and public health. KEY WORDS:Leishmania, zoonosis, dog, diagnosis, hemoculture, serology, polymerase chain reaction (PCR).

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Serodiagnosis of Sudanese visceral and mucosal leishmaniasis: comparison of ELISA-immunofluorescence and indirect haemagglutination

Cited by 31 publications

References 19 publications

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

In situ hybridisation for the detection of Leishmania species in paraffin wax‐embedded canine tissues using a digoxigenin‐labelled oligonucleotide probe

American visceral Leishmaniasis: a case report

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