Serodiagnosis of sporotrichosis infection in cats by enzyme-linked immunosorbent assay using a specific antigen, SsCBF, and crude exoantigens

Fernandes, Geisa Ferreira; Lópes-Bezerra, Leila M.; Bernardes-Engemann, Andréa Reis; Schubach, Tânia Maria Pacheco; Dias, Maria Adelaide Galvão; Pereira, Sandro Antônio; Camargo, Zoilo Pires de

doi:10.1016/j.vetmic.2010.07.007

Cited by 45 publications

(33 citation statements)

References 24 publications

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“…We also did not observe in the immunoassay described in this work differences in pattern recognition among patients with different clinical forms of sporotrichosis, in accordance to the results observed in the ELISA assay using mycelial form S. schenckii exoantigens 10 . Sensitivity and specificity values of the immunoblot test described in this work were calculated when a sample is considered positive if at least two proteins are reactive in the assay and are in accordance with other new described immunoassays for the serodiagnosis of sporotrichosis 10,30,31 …”

Section: Discussionsupporting

confidence: 89%

Cell‐free antigens of Sporothrix brasiliensis: antigenic diversity and application in an immunoblot assay

Almeida‐Paes¹,

Bailão²,

Pizzini³

et al. 2012

Mycoses

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Summary Sporotrichosis is a subcutaneous mycosis diagnosed by isolation of the fungus in culture. Serological tests for help in diagnosis in general do not use purified or recombinant antigens, because there is a paucity of described immunoreactive proteins, especially for the new described Sporothrix species, such as Sporothrix brasiliensis. This study aims to characterise antigens from S. brasiliensis and verify their application in serodiagnosis of sporotrichosis. An immunoblot assay allied with computer‐based analysis was used to identify putative antigenic molecules in a cell‐free extracts of both morphological phases of this fungus, and to delineate antigenic polymorphism among seven S. brasiliensis isolates and one S. schenckii Brazilian strain. The mycelial and yeast phase of the fungus originated 14 and 23 reactive bands, respectively, which were variable in intensity. An 85 kDa antigen, verified in the yeast phase of the fungus, was observed in all strains used and the immunodominant protein was identified. This protein, however, cross‐react with serum samples from patients infected with other pathogens. The results show that the S. brasiliensis cell‐free antigen extract is a single and inexpensive source of antigens, and can be applied on the sporotrichosis serodiagnosis.

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Section: Discussionsupporting

confidence: 89%

Cell‐free antigens of Sporothrix brasiliensis: antigenic diversity and application in an immunoblot assay

Almeida‐Paes¹,

Bailão²,

Pizzini³

et al. 2012

Mycoses

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“…These values are slightly higher than those for the concanavalin A binding fraction of the S. schenckii yeast cell wall (23), and similar observations were made with this purified antigen and crude exoantigens in the serodiagnosis of feline sporotrichosis. Purified antigens exhibited 90% sensitivity and 96% specificity, whereas crude exoantigens showed 96% sensitivity and 98% specificity (65). It has also been demonstrated that in order to improve the overall efficiency of antibody detection for diagnosis and follow-up of human sporotrichosis with the mycelial exoantigen preparation, a search for IgG and IgA antibodies for diagnosis and a search for IgG and IgM antibodies for follow-up purposes must be undertaken (6).…”

Section: Antibody Detectionmentioning

confidence: 99%

Sporothrix schenckii and Sporotrichosis

Barros

Paes²,

Schubach³

2011

Clin Microbiol Rev

491

633

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SUMMARY Sporotrichosis, which is caused by the dimorphic fungus Sporothrix schenckii , is currently distributed throughout the world, especially in tropical and subtropical zones. Infection generally occurs by traumatic inoculation of soil, plants, and organic matter contaminated with the fungus. Certain leisure and occupational activities, such as floriculture, agriculture, mining, and wood exploitation, are traditionally associated with the mycosis. Zoonotic transmission has been described in isolated cases or in small outbreaks. Since the end of the 1990s there has been an epidemic of sporotrichosis associated with transmission by cats in Rio de Janeiro, Brazil. More than 2,000 human cases and 3,000 animal cases have been reported. In humans, the lesions are usually restricted to the skin, subcutaneous cellular tissue, and adjacent lymphatic vessels. In cats, the disease can evolve with severe clinical manifestations and frequent systemic involvement. The gold standard for sporotrichosis diagnosis is culture. However, serological, histopathological, and molecular approaches have been recently adopted as auxiliary tools for the diagnosis of this mycotic infection. The first-choice treatment for both humans and cats is itraconazole.

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“…A similar observation was found when using this purified antigen and crude exoantigens for the serodiagnosis of feline sporotrichosis. Purified antigens showed 90% sensitivity and 96% specificity, whereas crude exoantigens presented 96% sensitivity and 98% specificity (Fernandes et al, 2011).…”

Section: Serologymentioning

confidence: 97%