Serodiagnosis of Schistosoma mansoni with Microsomal Adult Worm Antigen in an Enzyme-Linked Immunosorbent Assay using a Standard Curve Developed with a Reference Serum Pool *

We evaluated the performance of Western blot (WB) analysis using commercially available antigen strips and compared the results with those of indirect hemagglutination (IHA) and indirect immunofluorescence (IFAT) for the serodiagnosis of human schistosomiasis. The antigen preparation was a crude extract of Schistosoma mansoni. The WB profile characteristics of schistosomiasis were characterized by comparing the results for 58 serum samples from patients with parasitologically proven S. mansoni (n ‫؍‬ 12) and S. haematobium (n ‫؍‬ 46) infections and 37 individuals with probable cases of schistosomiasis but with only positive serology results. The specificity of WB analysis was assessed by testing 12 serum samples from healthy subjects, 67 serum samples from patients with other proven helminthic and protozoan infections, and 16 serum samples from patients with autoantibodies. Six immunodominant bands (65, 70, 80, 95, 110, and 120 kDa) were revealed with sera from patients with schistosomiasis. The presence of three or more bands in the range 65 to 120 kDa, with the exception of the 100-kDa band, was considered diagnostic for Schistosoma infection and had a specificity of 100% in our series. In patients with proven schistosomiasis, the sensitivity of WB analysis was 84.5%, whereas those of IFAT and IHA were 65.5 and 72.9%, respectively. For serologically proven cases, the sensitivity of WB analysis was 97.3%. The overall sensitivity and specificity for both groups of patients were 89.5 and 100%, respectively, with positive and negative predictive values of 100 and 91.3%, respectively. We conclude that WB analysis is a useful technique for the immunological diagnosis of schistosomiasis.

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Western Blot Kit for Diagnosis of Schistosomiasis

Sulahian

Garin

Izri

et al. 2005

“…Nonetheless, the diagnostic sensitivity for cases produced by other species is lower. The ELISA for MAMA permits the diagnosis of only 55 to 83% of the cases caused by S. haematobium (3,19). This fact limits the potential of using only one of these ELISAs for the diagnosis of imported schistosomiasis, especially among travelers and immigrants coming from Africa (9,26).…”

Section: Figmentioning

confidence: 99%

“…The purified antigens of adult worms have allowed the obtainment of microsomal antigens of S. mansoni (MAMA) and of S. haematobium with sensitivities of 96 and 98% for the diagnosis of schistosomiasis due to S. mansoni and S. haematobium, respectively (3,19). Nonetheless, the diagnostic sensitivity for cases produced by other species is lower.…”

Section: Figmentioning

confidence: 99%

Utility ofSchistosoma bovisAdult Worm Antigens for Diagnosis of Human Schistosomiasis by Enzyme-Linked Immunosorbent Assay and Electroimmunotransfer Blot Techniques

Pardo

Carranza

Turrientes

et al. 2004

Immunodiagnostic methods based on the detection of antibodies continue to be the most effective and practical methods for the diagnosis of imported schistosomiasis. Schistosoma bovis is a species whose final natural hosts are bovines, ovines, caprines, and small wild ruminants. Different studies have demonstrated the analogies existing between S. bovis and other Schistosoma species which affect humans. The objective of this work was to evaluate the utility of S. bovis adult worm antigens (AWA) for the diagnosis of imported human schistosomiasis by enzyme-linked immunosorbent assay (ELISA) and electroimmunotransfer blotting (EITB) techniques. By detecting eggs, the ELISA for S. bovis AWA was able to definitively detect imported cases with a sensitivity of 94%. The specificity of the ELISA for S. bovis AWA was 97%. There were no differences between the results of the S. bovis AWA ELISA for patients infected with Schistosoma mansoni and those infected with Schistosoma haematobium. The EITB technique showed bands of 85, 37, and 20 kDa, which are characteristic of infections with Schistosoma spp. Specific bands to indicate infection by different species of Schistosoma have not been detected. The combined use of the ELISA for S. bovis AWA and EITB increased the global sensitivity of the study to 97%. Our findings suggest that the ELISA for S. bovis AWA is a useful test for the immunodiagnosis of imported schistosomiasis and that EITB for detecting S. bovis AWA permits the confirmation of diagnosis when the ELISA for S. bovis AWA is positive.

“…Since the early 1980s, immunodiagnosis has been used to estimate infection rates and has been integrated into the Chinese control program with the goal of improving the diagnostic record in epidemiological surveys and identifying individuals to target for treatment. Nonetheless, cross-reaction is frequently a problem in immunodiagnostic assays, largely because of the use of crude antigens that are either intact material from the parasite or a soluble extract of the parasite or eggs, both of which contain many antigens that might be shared with unrelated pathogens (13,19). In addition, antibody-based serological assays do not discriminate between active and prior infections and cannot be used to evaluate therapeutic efficacy, because eggs can persist in the liver and gut and continue to stimulate the immune response, even after cure (21).…”

mentioning

confidence: 99%

Comparison of Recombinant Proteins fromSchistosoma japonicumfor Schistosomiasis Diagnosis

Jin

Zhou

et al. 2010

The most important animal reservoirs of Schistosoma japonicum in China are bovines. Diagnosis and control of bovine schistosomiasis is critical for reducing the prevalence of the disease. We screened defined diagnostic antigens that have the potential to increase the sensitivity and specificity of serological assays and to distinguish between active and prior infections. Five recombinant proteins with the potential to be diagnostic antigens were compared to the native soluble egg antigen preparation by enzyme-linked immunosorbent assay (ELISA). We evaluated the potentials of the recombinant proteins for discriminating active from prior infections, as well as the therapeutic efficacy of the established ELISA technique.