Serodiagnosis of <i>Helicobacter pylori</i> infection is not accurate for children aged below 10

Okuda, Masumi; Miyashiro, Eikichi; Koike, Masato; Tanaka, Tomoyuki; Bouoka, Mina; Okuda, Syuuji; Yoshikawa, Norishige

doi:10.1046/j.1442-200x.2002.01585.x

Cited by 40 publications

(37 citation statements)

References 7 publications

(14 reference statements)

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“…Sensitivity was found to be lower than that of the mothers and this could be attributed to the immature immune response or tolerance to H. pylori that exists in childhood and thus serodiagnosis of H. pylori infection is less useful in children. Similar findings were reported by Okuda et al (2002) who compared H. pylori IgG and IgA Abs with H. pylori stool Ags (Okuda et al, 2002). Our results were in concordance with that of Pourakbari et al (2013) who found that ELISA-IgG serum tests in children showed high specificity, but low sensitivity.…”

Section: Discussionsupporting

confidence: 92%

Diagnosis of Helicobacter pylori infection in children and their mothers using some non-invasive techniques

Mohamed¹,

Zalabany²,

Abaza³

et al. 2016

Afr. J. Microbiol. Res.

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Helicobacter pylori (H. pylori) is an important global public health concern. It infects approximately one half of the world's population. Considering the broad spectrum of available H. pylori diagnostic methods and since a reliable diagnosis is mandatory both before and after eradication therapy; thus it is crucial to study the different methods to be able to select the highly accurate laboratory tests that could be used to efficiently diagnose H. pylori infection in clinical practice. In this descriptive-cross sectional study we aimed to evaluate some non-invasive techniques for the diagnosis of H. pylori infection in children and their mothers. Forty children and their 21 mothers who had gastrointestinal symptoms were included. Stool, serum and saliva samples were collected from each enrolled child and mother to detect H. pylori antigens (Ags) in stool using chromatographic immunoassay and IgG antibodies (Abs) in serum and saliva by enzyme linked immune sorbent assay (ELISA). Collected data were analyzed using IBM SPSS software package version. Thirty (75%) children were positive for stool Ags, 10 (25%) were positive for serum Abs and only 5.4% were positive for saliva Abs. H. pylori stool Ag test was the most reliable test in all studied individuals and testing H. pylori IgG Abs in serum was more useful in adults than in children.

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Section: Discussionsupporting

confidence: 92%

Diagnosis of Helicobacter pylori infection in children and their mothers using some non-invasive techniques

Mohamed¹,

Zalabany²,

Abaza³

et al. 2016

Afr. J. Microbiol. Res.

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“…We reported similar results that serodiagnosis by HM-CAP EIA on Japanese asymptomatic children under 10 years of age had 51.4% sensitivity and 97.2% specificity in reference to H. pylori stool antigen test (HpSA test) (20). In contrast, a commercially available urine-based EIA test, URINELISA, is highly reliable with 92.8% sensitivity and 95.8% specificity in reference to the 13 C-labeled urea breath test (UBT) and HpSA test on the same age group of a similar population (21).…”

supporting

confidence: 57%

A Strain-Specific Antigen in JapaneseHelicobacter pyloriRecognized in Sera of Japanese Children

Okuda

Sugiyama

Fukunaga

et al. 2005

Clin Vaccine Immunol

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An enzyme immuno assay (EIA) test based on Japanese strain-derived high-molecular-weight cell-associated proteins (JHM-CAP) was evaluated by comparing with a previously developed EIA test based on a U.S. strain-derived high-molecular-weight cell-associated proteins (HM-CAP). Serum samples of 131 Japanese asymptomatic children (mean age, 5.5 years; range, 0 to 21 years) were tested that include 43 positive and 88 negative children as judged by Helicobacter pylori stool antigen test (HpSA test). Both tests showed comparable and reliable specificities, but the sensitivity of JHM-CAP EIA, at 93.0%, was much higher than that of HM-CAP EIA, at 67.4%. More false-negative results of HM-CAP were obtained in children under 10 years of age. Immunoblot analysis revealed that the JHM-CAP but not the HM-CAP preparation had a 100-kDa antigen recognized by JHM-CAP positive sera. It was concluded that JHM-CAP EIA is highly accurate for the serodiagnosis of H. pylori infection in Japanese young children and that the high sensitivity of JHM-CAP EIA in contrast to HM-CAP EIA is due to the presence of a 100-kDa antigen in Japanese strains that may be recognized by the host immune system at an early stage of infection.

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“…The serologic test for H. pylori infection can be easily performed in epidemiologic studies using stored sera. However, concerns regarding validity have been raised due to its lower sensitivity and specificity as compared with the HpSA tests and urea breath tests [30]. On the other hand, other recent studies have reported high accuracy of the serum antibody test in children.…”

Section: Discussionmentioning

confidence: 99%

“…One factor affecting the results may have been the age of participants in the current study. Previous reports have stated that serum antibody tests are not useful in children younger than 10 years because their immune response is immature [24, 30]. In one study, which examined serum H. pylori antibodies using the ELISA method in 157 children (mean age 5.6 ± 4.6 years), the test was found to have a sensitivity of 54.5% in children under the age of 10 years and a sensitivity of 100% in those aged 10 years and above [19].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Accuracy of Latex Agglutination Turbidimetric Immunoassay in Screening Adolescents for Helicobacter pylori Infection in Japan

et al. 2018

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Background/Aims: Serologic tests are commonly used for screening Helicobacter pylori infection because they not only provide quick results but also are inexpensive. A new latex agglutination serum antibody assay (LZ test) has been developed and it is expected to be as effective as conventional assays. This study aimed to calculate a reliable cutoff value for the LZ test and to estimate the sensitivity and specificity of the cutoff value in screening adolescents for H. pylori infection in Japan. Methods: We screened junior high school students in Akita Prefecture, Japan, for H. pylori infection. We used the data of 213 such students who underwent H. pylori stool antigen (HpSA) tests in 2016. The students who had positive results with HpSA tests were diagnosed with H. pylori infection. Of the 213 students, 209 underwent the LZ test. Results: The prevalence rate of H. pylori infection was 3.8% (8/209). The area under the curve for the LZ test was 0.88. The cutoff value of the LZ test was determined to be 3.1 U/mL. At this value, the sensitivity and specificity were 87.5 and 91.5%, respectively. Conclusion: The accuracy of the LZ test in adolescents was well balanced for sensitivity and specificity as well as for tolerable results.

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Serodiagnosis of Helicobacter pylori infection is not accurate for children aged below 10

Abstract: These facts suggested that an immature immune response or tolerance to H. pylori exists in childhood and serodiagnosis of H. pylori infection is less useful in children aged below 10.

Cited by 40 publications

References 7 publications

Diagnosis of Helicobacter pylori infection in children and their mothers using some non-invasive techniques

Diagnosis of Helicobacter pylori infection in children and their mothers using some non-invasive techniques

A Strain-Specific Antigen in JapaneseHelicobacter pyloriRecognized in Sera of Japanese Children

Diagnostic Accuracy of Latex Agglutination Turbidimetric Immunoassay in Screening Adolescents for Helicobacter pylori Infection in Japan

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