Serodiagnosis of cutaneous leishmaniasis in Jordan using indirect fluorescent antibody test and the enzyme-linked immunosorbent assay

Mosleh, Ibrahim M.; Saliba, Elias K.; Al-Khateeb, M.; Bisharat, Zuhair; Oumeish, Oumeish Y.; Bitar, Waleed

doi:10.1016/0001-706x(94)00083-d

Cited by 12 publications

(9 citation statements)

References 20 publications

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“…This result agrees with previous studies that also demonstrated an increased sensitivity of the assays when homologous instead of heterologous antigens were used (25)(26)(27).…”

Section: Discussionsupporting

confidence: 93%

Comparative study of amplification systems in immunoenzyme assays for the diagnosis of American tegumentary leishmaniasis

Nascimento

Passos

Mouta-Confort

et al. 2009

Clinical Laboratory Analysis

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We compared the accuracy and reliability of three amplification systems for enzyme immunoassays in the detection of specific IgG antibodies for the diagnosis of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis in patients from an endemic area in Rio de Janeiro, Brazil. Partially soluble antigens obtained from the promastigote forms of L. (V.) braziliensis were used. For development of the reaction, two chromogens, 1,2-orthophenylenediamine (OPD) and 3,3',5,5'-tetramethylbenzidine (TMB), and a fluorogen, 4-methylumbelliferylphosphate (MUP), were tested. The performance of each system was compared using the following parameters: accuracy, intraclass correlation coefficient (ICC), and area under the receiver operating characteristic (ROC) curve. Sensitivity was the same (97.4%) for all systems. The reliability was excellent (ICC = 98.6, 98.7, and 99.1%) and specificity was 93.7, 95.8, and 97.4% for OPD, MUP, and TMB, respectively, showing no statistical significance. Despite the absence of differences in the performance of the three systems, the use of TMB is suggested because of its operational advantages, such as low cost compared with fluorogens, easy manipulation, greater stability, and lower toxicity.

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“…This result agrees with previous studies that also demonstrated an increased sensitivity of the assays when homologous instead of heterologous antigens were used (25)(26)(27).…”

Section: Discussionsupporting

confidence: 93%

Comparative study of amplification systems in immunoenzyme assays for the diagnosis of American tegumentary leishmaniasis

Nascimento

Passos

Mouta-Confort

et al. 2009

Clinical Laboratory Analysis

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“…The use of these serologic tools in diagnosis of CL has also been evaluated in a few of studies [23–25]. Nevertheless, no serologic test is yet available for routine diagnosis of CL.…”

Section: Discussionmentioning

confidence: 99%

“…Lower sensitivity has been reported by Mosleh (81%) and also by Monroy-Ostria (85.4%) for IFA [23, 26]. In these studies, the cut-off point has been set on 1 : 16 titer and we used a same cutoff for IFA.…”

Section: Discussionmentioning

confidence: 99%

Performance of an ELISA and Indirect Immunofluorescence Assay in Serological Diagnosis of Zoonotic Cutaneous Leishmaniasis in Iran

Sarkari

Ashrafmansouri

Hatam

et al. 2014

Interdisciplinary Perspectives on Infectious Diseases

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Serological assays have been extensively evaluated for diagnosis of visceral leishmaniasis (VL) and considered as a routine method for diagnosis of VL while these methods are not properly evaluated for diagnosis of cutaneous leishmaniasis (CL). This study aimed to assess the performance of indirect immunofluorescent-antibody test (IFA) and enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of cutaneous leishmaniasis in Iran. Sixty-one sera samples from parasitologically confirmed CL patients and 50 sera from healthy controls along with 50 sera from non-CL patients were collected. Antigen was prepared from promastigotes and amastigotes of Leishmania major. IFA was used to detect anti-Leishmania IgG while ELISA was used to detect anti-Leishmania IgM, total IgG, or IgG subclasses (IgG1 and 4). ELISA, for detection of total IgG and IgM, showed sensitivity of 83.6% and 84.7% and specificity of 62.7% and 54.6%, respectively. Sensitivity and specificity of ELISA for detecting IgG1 and IgG4 were 64%, 75% and 85%, 49%, respectively. Sensitivity and specificity of IFA were 91.6% and 81%. Conclusion. Findings of this study demonstrated that serological test, especially IFA, can be used for proper diagnosis of CL.

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“…We decided to do a comparative analysis of test performance with the proposed technique IFA, the most conventional serological technique used for detection of antibodies indicated in the diagnosis of ATL by L. (V.) braziliensis. Although, in some situations, the IFA serological results present difficulties on finding parasites (Sampaio et al, 2009;Mosleh et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…Studies related to the analysis of the humoral immune response in ATL have approached the role of immunoglobulins in immunopathologic mechanisms involved on the resistance and/or pathogenesis of the infection. In addition, they have also evaluated the use of serological investigations in diagnosis and monitoring the post-treatment efficacy (O'Neil et al, 1993;Mosleh et al, 1995;Chiari et al, 1973). Studies have shown that all anti-Leishmania specific antibodies isotypes, except IgD, are detected in the serum of patients with ATL.…”

Section: Introductionmentioning

confidence: 99%

Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G

Oliveira

Castro

Almeida

et al. 2013

Journal of Immunological Methods

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Serodiagnosis of cutaneous leishmaniasis in Jordan using indirect fluorescent antibody test and the enzyme-linked immunosorbent assay

Cited by 12 publications

References 20 publications

Comparative study of amplification systems in immunoenzyme assays for the diagnosis of American tegumentary leishmaniasis

Comparative study of amplification systems in immunoenzyme assays for the diagnosis of American tegumentary leishmaniasis

Performance of an ELISA and Indirect Immunofluorescence Assay in Serological Diagnosis of Zoonotic Cutaneous Leishmaniasis in Iran

Comparison of flow cytometry and indirect immunofluorescence assay in the diagnosis and cure criterion after therapy of American tegumentary leishmaniasis by anti-live Leishmania (Viannia) braziliensis immunoglobulin G

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