Serodiagnosis of canine visceral leishmaniasis in Portugal: comparison of three methods

Rachamim, Nurit; Jaffe, Charles L.; Abranches, P.; Silva-Pereira, M. C. D.; Schnur, Lionel F.; Jacobson, Raymond L.

doi:10.1080/00034983.1991.11812600

Cited by 20 publications

(10 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These antigens may be the same as those of 14 and 16kDa described in another recent study (Mary et al 1992). In a study using L. donovani membranes, common antigens of 26 and 70-84kDa were recognized by all the 43 L. infuntum-infected dogs sera tested (Rachamin et al 1991). We suggest that a combination of several of these selected antigens should be employed, as each may contribute to the immunodiagnosis of human visceral leishmaniasis.…”

Section: Infanrum Lem 497 Polypeptides Separated By 10% Polyacrylasupporting

confidence: 52%

Identification of a 94‐kilodalton antigen on Leishmania promastigote forms and its specific recognition in human and canine visceral leishmaniasis

Rolland¹,

Zilberfarb

Furtado

et al. 1994

Parasite Immunology

View full text Add to dashboard Cite

We have analysed by immunoblotting sera from humans and dogs with visceral leishmaniasis, from the Old World as well as the New. When lysates of promastigotes are used as antigens, antibodies against a 94 kDa Leishmania component are detected, regardless of the age and geographical origin of the patient, the serum antibody titre as measured by indirect immunofluorescence, and the number of arcs in counterimmunoelectrophoresis. Low dilutions of sera from patients with Old and New World cutaneous leishmaniasis did not react with the 94-kDa antigen, whatever the species of Leishmania used as antigens. Sera from patients with other infections than leishmaniases, or without infection, are negative, even at low dilution. Anti-94 kDa antibodies were detected in the sera of Leishmania-infected dogs from both the Old and the New World. When lysates of Leishmania mexicana axenic amastigotes are used as antigens, the 94-kDa antigen was little or none identified by sera from humans and dogs with visceral leishmaniasis, and never recognized by control sera. Thus, the specific recognition of the 94-kDa promastigote antigen in human and canine visceral leishmaniasis suggests that this antigen could be a potential candidate in the differential immunodiagnosis of the disease.

show abstract

Section: Infanrum Lem 497 Polypeptides Separated By 10% Polyacrylasupporting

confidence: 52%

Identification of a 94‐kilodalton antigen on Leishmania promastigote forms and its specific recognition in human and canine visceral leishmaniasis

Rolland¹,

Zilberfarb

Furtado

et al. 1994

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…In contrast with the results reported by Rachamim and Jaffe (1991) but in agreement with Mancianti and others (1995), the end titres of the various methods were positively correlated. Although Rachamim and Jaffe (1991) used a conventional ELISA system, one can assume that both the conventional and the slide ELISA detect the same antibodies.…”

Section: Discussionsupporting

confidence: 87%

“…Although Rachamim and Jaffe (1991) used a conventional ELISA system, one can assume that both the conventional and the slide ELISA detect the same antibodies. Indeed, the only difference between the two ELISA methods is the substrate conversions: in the conventional ELISA a soluble end product of the enzymatic reaction is formed, whereas in the slide ELISA an insoluble precipitate is formed.…”

Section: Discussionmentioning

confidence: 99%

Abstracts

1997

Veterinary Record

View full text Add to dashboard Cite

it proved to be a better blocking agent of non-specific reactions.Diluted SELISA conjugate lost its reactivity after a few days. It should therefore be diluted just before use, because it is critical to work at the optimal concentration of conjugate in this test.Of the 32 dog sera, 20 were positive with median titres of 1/1024, 1/10240 and 1/204,800 for the IFAT, SELISA and DAT, respectively. The IFAT and SELISA each had one borderline value. One of the sera in the DAT gave a false positive result, but the 12 'negative' sera remained negative in the four other tests. Crossreactions were observed in the mID with the two Trypanosomainfected dogs, but were absent from all the other tests with dogs with heterologous infections.The iEp showed a mean of 4-2 arcs, with arcs 4 and/or 24 being recognised in all the 19 mID-positive sera. In 22 per cent only arc 4 was detected and in 28 per cent only arc 24. Both arcs were detected in 53 per cent of the positive samples, resulting in an overall detection rate of arc 4 in 75 per cent and arc 24 in 81 per cent of the sera.The Spearman correlation (r,) (Siegel 1956) was used to compare the ranked maximum titre given by the different tests. There were significant positive correlations of rs = 0.67 between the IFAT and the SELISA (P<0-01) and rs = 0.46 between the IFAT and the DAT (P<0.05). Concordance of the three tests was assessed with the Kendall coefficient of concordance (W) (Siegel 1956), which had a value of 0-63 (P=0-01 1).

show abstract

“…According to Gardiner et al (1984), the identification of highly specific antigens of the Leishmania species would be desirable for the rapid diagnosis of the infection. Of note, highly specific antigens of Leishmania have been identified by immunoblotting (Aisa et al, 1998; Da Costa et al., 1996; Jaffe and Zalis, 1988; Rachamim et al, 1991; Rami et al, 2005). In this study, we described highly specific proteins for potential use in CVL diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Identification of Highly Specific and Cross‐Reactive Antigens of Leishmania Species by Antibodies from Leishmania (Leishmania) chagasi Naturally Infected Dogs

Vale

Fujiwara

Neto³

et al. 2009

Zoonoses and Public Health

View full text Add to dashboard Cite

The Leishmania species present a genetic homology that ranges from 69 to 90%. Because of this homology, heterologous antigens have been used in the immunodiagnosis and vaccine development against Leishmania infections. In the current work, we describe the identification of species-specific and cross-reactive antigens among several New World Leishmania species, using symptomatic and asymptomatic naturally Leishmania chagasi-infected dog sera. Soluble antigens from five strains of New World Leishmania were separated by electrophoresis in SDS-PAGE and immunoblotted. Different proteins were uniquely recognized in the L. chagasi panel by either symptomatic or asymptomatic dog sera suggesting their use as markers for the progression of disease and diagnosis of the initial (sub-clinical) phase of the infection. Cross-reactive antigens were identified using heterologous antigenic panels (L. amazonensis strains PH8 and BH6, L. guyanensis and L. braziliensis). L. guyanensis panel showed the highest cross-reactivity against L. chagasi specific antibodies, suggesting that proteins from this extract might be suitable for the diagnosis of visceral canine leishmaniasis. Interestingly, the 51 and 97 kDa proteins of Leishmania were widely recognized (77.8% to 100%) among all antigenic panels tested, supporting their potential use for immunodiagnosis. Finally, we identified several leishmanial antigens that might be useful for routine diagnosis and seroepidemiological studies of the visceral canine leishmaniasis.

show abstract

Serodiagnosis of canine visceral leishmaniasis in Portugal: comparison of three methods

Cited by 20 publications

References 11 publications

Identification of a 94‐kilodalton antigen on Leishmania promastigote forms and its specific recognition in human and canine visceral leishmaniasis

Identification of a 94‐kilodalton antigen on Leishmania promastigote forms and its specific recognition in human and canine visceral leishmaniasis

Abstracts

Identification of Highly Specific and Cross‐Reactive Antigens of Leishmania Species by Antibodies from Leishmania (Leishmania) chagasi Naturally Infected Dogs

Contact Info

Product

Resources

About