Serious disease outbreaks caused by viruses transmitted by Aedes aegypti in Brazil

Figueiredo, Luíz Tadeu Moraes

doi:10.1590/0037-8682-0209-2016

Cited by 5 publications

(4 citation statements)

References 9 publications

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“…and Aedes sp. act as vectors of MAYV in rural and urban areas, respectively [8]. A recent nding shows the potential for MAYV transmission by the urban vectors Aedes aegypti and Aedes albopictus thus contributing to the classi cation of the MAYV as an emerging virus, having a potential for inclusion in the urban cycle, as occurred more recently with the Chikungunya virus [9][10][11].…”

Section: Mayaro Virus (Mayv) Belongs To the Togaviridae Family Alphamentioning

confidence: 99%

Virucidal and Antiviral Activity of Extract of Punica Granatum Cultivated in Brazil

Salles¹,

Meneses²,

Caldas³

et al. 2021

Preprint

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Background: The arthropod-borne Mayaro virus (MAYV) causes ‘Mayaro fever’, a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. Currently, there are no licensed drugs against most mosquito-transmitted viruses. Punica granatum (pomegranate) fruits cultivated in Brazil were submitted to a phytochemical investigation for the identification and isolation of antiviral compounds. In the present study we studied the antiviral activity of pomegranate extracts in Vero cells infected with Mayaro virus. Methods: The Ethanol extract and Punicalagin of Pomegranate were extracted solely of the shell and purified by chromatographic fractionation and chemically identified using spectroscopic techniques. Cytotoxicity of purified compounds was measured by the dye-uptake assay while their antiviral activity was evaluated by a virus yield inhibition assay.Results: Pomegranate ethanol extract (CC50 = 588.9, IC50 = 12.3) and a fraction containing punicalagin as major compound (CC50 = 441.5, IC50 = 28.2) were shown to have antiviral activity (SI 49 and 16, respectively) against Mayaro virus, an alphavirus. Immunofluorescence analysis showed the virucidal effect of Pomegranate extract and Transmission Electron Microscopy (TEM) revealed damage in viral particles treated with this extract. Conclusions: The P. granatum extract is a promising source of antiviral compounds against the alphavirus MAYV and are excellent candidates for future studies with other enveloped RNA viruses.

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Section: Mayaro Virus (Mayv) Belongs To the Togaviridae Family Alphamentioning

confidence: 99%

Virucidal and Antiviral Activity of Extract of Punica Granatum Cultivated in Brazil

Salles¹,

Meneses²,

Caldas³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…and Aedes spp. act as vectors of MAYV in rural and urban areas, respectively [ 8 ]. A recent finding shows the potential for MAYV transmission by the urban vectors Aedes aegypti and Aedes albopictus , thus contributing to the classification of the MAYV as an emerging virus, with the potential for inclusion in the urban cycle, as has occurred more recently with the Chikungunya virus [ 9 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

Virucidal and antiviral activities of pomegranate (Punica granatum) extract against the mosquito-borne Mayaro virus

et al. 2021

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Background The arthropod-borne Mayaro virus (MAYV) causes “Mayaro fever,” a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. There are currently no licensed drugs against most mosquito-transmitted viruses. Punica granatum (pomegranate) fruits cultivated in Brazil have been subjected to phytochemical investigation for the identification and isolation of antiviral compounds. In the present study, we explored the antiviral activity of pomegranate extracts in Vero cells infected with Mayaro virus. Methods The ethanol extract and punicalagin of pomegranate were extracted solely from the shell and purified by chromatographic fractionation, and were chemically identified using spectroscopic techniques. The cytotoxicity of the purified compounds was measured by the dye uptake assay, while their antiviral activity was evaluated by a virus yield inhibition assay. Results Pomegranate ethanol extract (CC50 = 588.9, IC50 = 12.3) and a fraction containing punicalagin as major compound (CC50 = 441.5, IC50 = 28.2) were shown to have antiviral activity (SI 49 and 16, respectively) against Mayaro virus, an alphavirus. Immunofluorescence analysis showed the virucidal effect of pomegranate extract, and transmission electron microscopy (TEM) revealed damage in viral particles treated with this extract. Conclusions The P. granatum extract is a promising source of antiviral compounds against the alphavirus MAYV and represents an excellent candidate for future studies with other enveloped RNA viruses. Graphical abstract

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“…Currently, DENV is the most important arthropod-borne viral disease worldwide, with an estimated 50 million infections occurring each year, which may be asymptomatic as in the majority of cases or may result in severe hemorrhagic fevers 4 . In Brazil, all four DENV serotypes have been reported in highly populated areas in the last three decades, such as DENV-1 in 1985, DENV-2 in 1990, DENV-3 in 2000, and DENV-4 in 2008 5,6 . These viruses have spread and have been reintroduced probably several times, subsequently producing large outbreaks that affect at least ten million people, causing severe disease in tens of thousands of people, eventually leading to thousands of deaths 5 .…”

Section: Introductionmentioning

confidence: 99%

Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells

Barreira

Scheucher

Romeiro

et al. 2018

Rev. Soc. Bras. Med. Trop.

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Introduction: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. Methods: In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR). Results: All three assays identified 29.1% (39/134) of the patients as denguepositive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods. Conclusions: The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.

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Serious disease outbreaks caused by viruses transmitted by Aedes aegypti in Brazil

Cited by 5 publications

References 9 publications

Virucidal and Antiviral Activity of Extract of Punica Granatum Cultivated in Brazil

Virucidal and Antiviral Activity of Extract of Punica Granatum Cultivated in Brazil

Virucidal and antiviral activities of pomegranate (Punica granatum) extract against the mosquito-borne Mayaro virus

Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells

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