Serine/threonine-kinase 33 (Stk33) – Component of the neuroendocrine network?

Reuss, Stefan; Brauksiepe, Bastienne; Disque-Kaiser, Ursula; Olivier, Tim L.

doi:10.1016/j.brainres.2016.11.006

Cited by 8 publications

(8 citation statements)

References 28 publications

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“…STK33 gene encodes a novel serine/threonine protein kinase. Due to the specific structure, STK33 cannot only activate the protease but also participate in a variety of life activities by affecting absorption of calcium ( 15 ). STK33, as a newly discovered gene with unclear mechanism, has attracted increasing attention.…”

Section: Discussionmentioning

confidence: 99%

Correlation between STK33 and the pathology and prognosis of lung cancer

Tang

Zhang

et al. 2017

Oncology Letters

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Correlation between the expression of STK33 and the pathology of lung cancer was investigated, to explore its effects on prognosis. Hundred and two lung cancer patients diagnosed by pathological examinations were randomly selected in Shanghai Jiao Tong University Affiliated Sixth People's Hospital from February, 2012 to February, 2017 to serve as observation group, and the tumor tissues were collected. At the same time, 19 patients with lung benign lesions were selected and lung tissues were also collected to serve as control group. RT-qPCR was used to detect the expression of STK33 mRNA in tissues. Expression levels of STK33 protein were detected and compared by SP immunohistochemistry staining and western blot analysis. Statistical analysis was performed to analyze the correlation between STK33 expression and the pathology and prognosis of lung cancer. Results of PCR showed that expression level of STK33 gene in control group was significantly lower than that in observation group (p<0.05). The expression level of STK33 mRNA in lung adenocarcinoma and squamous cell carcinoma was lower than that in lung small cell carcinoma and large cell carcinoma (p<0.05). Western blot analysis showed that the expression level of STK33 protein in lung small cell carcinoma and large cell carcinoma was significantly higher than that in lung adenocarcinoma and squamous cell carcinoma (p<0.05). Immunohistochemistry staining showed that the positive rate of STK33 in lung large cell carcinoma (100%) and small cell carcinoma (100%) was significantly higher than that in lung adenocarcinoma (88.1%) and squamous cell carcinoma (86.2%) (p<0.05). The 5-year survival rate analysis showed that the recurrence-free survival rate and overall survival rate of STK33 gene high expression level group were significantly lower than those of low expression level group (p<0.05). The differential expression level of STK33 is related to the pathology and prognosis of lung cancer, which is of great value in clinical diagnosis and prognosis evaluation.

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Section: Discussionmentioning

confidence: 99%

Correlation between STK33 and the pathology and prognosis of lung cancer

Tang

Zhang

et al. 2017

Oncology Letters

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“…STK33 belongs to a member of the calcium/calmodulin‐dependent kinase (CAMK), which is mainly existed in testis, certain brain regions and embryonic organs such as brain, heart and spinal cord in human and mouse and is likely to participate in spermatogenesis and organ ontogenesis . Studies show that STK33 is co‐localized with the intermediate filament protein vimentin in tanycytes of mouse, rat and hamster, which is involved in the dynamic changes of the intermediate filament cytoskeleton assembly/disassembly, and regulates the cell structure as well as a few important functions through the specific phosphorylation of vimentin . It is well known that apoptosis caused by STK33 suppression is mediated by the mitochondrial pathway, PI3K/AKT cascade or the mitogen‐activated protein kinases (MAPKs)‐signalling pathway …”

Section: Introductionmentioning

confidence: 99%

“…7,8 Studies show that STK33 is co-localized with the intermediate filament protein vimentin in tanycytes of mouse, rat and hamster, 9 which is involved in the dynamic changes of the intermediate filament cytoskeleton assembly/disassembly, and regulates the cell structure as well as a few important functions through the specific phosphorylation of vimentin. 10,11 It is well known that apoptosis caused by STK33 suppression is mediated by the mitochondrial pathway, PI3K/AKT cascade or the mitogen-activated protein kinases (MAPKs)-signalling pathway. 6 It has been documented that MAPKs, which are composed of a serine/threonine protein kinases, represent the classical signal transduction pathways.…”

Section: Introductionmentioning

confidence: 99%

STK33 alleviates gentamicin‐induced ototoxicity in cochlear hair cells and House Ear Institute‐Organ of Corti 1 cells

Zhou

Sun

Zhang

et al. 2018

J Cellular Molecular Medi

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Serine/threonine kinase 33 (STK33), a member of the calcium/calmodulin‐dependent kinase (CAMK), plays vital roles in a wide spectrum of cell processes. The present study was designed to investigate whether STK33 expressed in the mammalian cochlea and, if so, what effect STK33 exerted on aminoglycoside‐induced ototoxicity in House Ear Institute‐Organ of Corti 1 (HEI‐OC1) cells. Immunofluorescence staining and western blotting were performed to investigate STK33 expression in cochlear hair cells (HCs) and HEI‐OC1 cells with or without gentamicin treatment. CCK8, flow cytometry, immunofluorescence staining and western blotting were employed to detect the effects of STK33 knockdown, and/or U0126, and/or N‐acetyl‐L‐cysteine (NAC) on the sensitivity to gentamicin‐induced ototoxicity in HEI‐OC1 cells. We found that STK33 was expressed in both mice cochlear HCs and HEI‐OC1 cells, and the expression of STK33 was significantly decreased in cochlear HCs and HEI‐OC1 cells after gentamicin exposure. STK33 knockdown resulted in an increase in the cleaved caspase‐3 and Bax expressions as well as cell apoptosis after gentamicin damage in HEI‐OC1 cells. Mechanistic studies revealed that knockdown of STK33 led to activated mitochondrial apoptosis pathway as well as augmented reactive oxygen species (ROS) accumulation after gentamicin damage. Moreover, STK33 was involved in extracellular signal‐regulated kinase 1/2 pathway in primary culture of HCs and HEI‐OC1 cells in response to gentamicin insult. The findings from this work indicate that STK33 decreases the sensitivity to the apoptosis dependent on mitochondrial apoptotic pathway by regulating ROS generation after gentamicin treatment, which provides a new potential target for protection from the aminoglycoside‐induced ototoxicity.

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“…STK33 is located on chromosome region 11p15.3; its entire functions have not been elucidated yet; however, there are some suggestions (Brauksiepe, Baumgarten, Reuss, & Schmidt, ). For example, Reuss, Brauksiepe, Disque‐Kaiser, & Olivier, evaluated the expression and presence of STK33 protein in neuronal structures of central nervous system in rats and hamsters and observed large STK33‐immunoreactive axonal projections from magnocellular hypothalamus to the neurohypophysis (Reuss et al, ). Hence, this functional connection suggests a bridge from neuronal to humoral regulation of the endocrine system.…”

Section: Discussionmentioning

confidence: 99%

Gene‐level genome‐wide association analysis of suicide attempt, a preliminary study in a psychiatric Mexican population

González‐Castro

Martínez‐Magaña

Tovilla‐Zárate

et al. 2019

Molec Gen & Gen Med

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BackgroundEvidence suggests that liability for suicide behavior is heritable; additionally, suicide has been partly related to other psychiatric disorders. Nevertheless, most of the information reported so far address Caucasian and Asian individuals. Hence, our aim was to conduct a gene‐level association study in Mexican psychiatric individuals diagnosed with suicide attempt.MethodsWe recruited 192 individuals from two clinical centers in Mexico. All participants were born in Mexico and had Mexican parents and grandparents. Direct genotyping was performed using the commercial platform Infinium PsychArray BeadChip. A p‐value lower than 1e‐05 was considered as gene‐level significant and a p‐value lower than 1e‐04 was considered as gene‐level nominal significant.ResultsOur analyses showed that SCARA5 was associated to suicide intent at a gene‐level with statistical significance (p‐value = 1.12e‐6). Other genes were nominally associated with suicide attempt: GHSR (p‐value = 0.0004), RGS10 (p‐value = 5.13e‐5), and STK33 (p‐value = 3.62e‐5). Regarding gene variant analyses, the SNPs with a statistical association (p > .05) were rs561361616, rs1537577, rs11198999 for RGS10, and rs11041981, rs11041993, rs11041994, rs11041995, rs11041997, rs10840083, rs10769918 for STK33. For these genes, previous studies have associated SCARA5 with depression, GHSR with alcohol dependence and depression, and RGS10 with schizophrenia and depression. To date, STK33 has not been associated with any psychiatric disorder.ConclusionOur outcomes revealed that SCARA5, GHSR, RGS10 and STK33 could be considered as risk biomarkers for suicide attempt behavior in our Mexican psychiatric sample. We recommend to perform larger scale analyses to have conclusive results.

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Serine/threonine-kinase 33 (Stk33) – Component of the neuroendocrine network?

Cited by 8 publications

References 28 publications

Correlation between STK33 and the pathology and prognosis of lung cancer

Correlation between STK33 and the pathology and prognosis of lung cancer

STK33 alleviates gentamicin‐induced ototoxicity in cochlear hair cells and House Ear Institute‐Organ of Corti 1 cells

Gene‐level genome‐wide association analysis of suicide attempt, a preliminary study in a psychiatric Mexican population

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