Serine Protease of Trichinella Spiralis and Its Possible Application in the Early Diagnosis of Trichinellosis in Animals

Abstract

Serine proteases of parasites play a significant role in the infection of a host organism. This is especially reflected in the process of immunosuppression, in particular, during IgG hydrolysis. The importance of the performed functionensures their presence at all stages of development, which makes them key targets for early diagnosis of trichinellosis. In this study, we demonstrated that serineprotease transcripts are detected in 83% of cases when mice are infected with BALB/c on days 7 and 14 after infection and are identified by molecular methods.Two groups of mice were infected with different doses of larvae (100 and 250 larvae), it was shown that the dosage did not affect the effectiveness of serine protease detection in mice, which would provide a better diagnostic effect. The transcripts presence confirms the possibility of using serine protease as a protein for the diagnosis of trichinellosis in animals and humans.