Serine protease inhibition and modulatory-antibiotic activity of the proteic extract and fractions from Amburana cearensis

Oliveira, Maria T.A.; Moura, Geovanna Maria de Medeiros; Cruz, Joelton Igor Oliveira da; Lima, Rayanna V.C.; Santos, Elisama Vieira dos; Andrade, Jacqueline Cosmo; Alencar, Marcus Viní­cius Oliveira Barros de; Landim, Vicente P.A.; Coutinho, Henrique Douglas Melo; Uchôa, Adriana Ferreira

doi:10.1016/j.fct.2019.110946

Cited by 9 publications

(6 citation statements)

References 28 publications

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“…In a review paper on lesions of the oral mucosa, several plant extracts, including Matricaria recutita L. and Aloe vera (L.) Burm.f., presented compounds capable of acting on certain bacterial strains (5). In addition, plant extracts and substances derived from these extracts are capable of presenting antibiotic adjuvant activities, increasing their antibacterial potential, when added in subinhibitory doses (6,7,8,9,10).…”

Section: Introductionmentioning

confidence: 99%

UPLC-QTOF-MS/MS analysis and antibacterial activity of the Manilkara zapota (L.) P. Royen against Escherichia coli and other MDR bacteria

Freitas

Campina

Costa

et al. 2021

Cell Mol Biol (Noisy-le-grand)

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With the spread of bacterial resistance against clinically used antibiotics, natural plant-derived products are being studied as new sources of antibacterial molecules. Manilkara zapota is a common plant species in the American continent that is used as a food source. Studies show the M. zapota extract is rich in phenolic substances that can serve as basic molecules for the pharmaceutical industry. An extract from fresh M. zapota leaves was produced and tested to identify the compounds present, as well as its direct antibacterial and clinical antibiotic modulatory activities. To analyze the results, a new statistical methodology based on the Shannon-Wiener index was tested, capable of correcting distortions in heterogeneous environments. The Hydroethanolic Extract of Manilkara zapota leaves (HEMzL) presented a wide variety of phenolic products, as well as tannins, in the UPLC analysis. The extract showed direct antibacterial activity against the standard Staphylococcus aureus strain, however, it either acted antagonistically when associated with the tested antibiotics, or it did not present statistical significance when compared to the control. This demonstrates a need to be cautious when associating natural products with antibiotics for clinical use, as a hindrance to infectious treatments may occur. As for the statistical analysis mechanism tested, this proved to be effective, reducing false negatives at low antibiotic concentrations and false positives at high concentrations in the microdilution plate.

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Section: Introductionmentioning

confidence: 99%

UPLC-QTOF-MS/MS analysis and antibacterial activity of the Manilkara zapota (L.) P. Royen against Escherichia coli and other MDR bacteria

Freitas

Campina

Costa

et al. 2021

Cell Mol Biol (Noisy-le-grand)

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“…Recently, Oliveira et al, 2020 [ 31 ] have shown a potential synergistic antibacterial effect of total protein extract of A. cearensis seeds and three fractions obtained from precipitation with different ammonium sulfate concentrations against Staphylococcus aureus and Escherichia coli strains, together with known antibacterial drugs (norfloxacin, penicillin and gentamicin). The crude extract, together with the three antibiotics, inhibited S. aureus and E. coli growth.…”

Section: Biological Activitiesmentioning

confidence: 99%

Amburana cearensis: Pharmacological and Neuroprotective Effects of Its Compounds

et al. 2020

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Amburana cearensis A.C. Smith is an endemic tree from Northeastern Brazil used in folk medicine as teas, decocts and syrups for the treatment of various respiratory and inflammatory diseases, since therapeutic properties have been attributed to compounds from its stem bark and seeds. Numerous pharmacological properties of semi-purified extracts and isolated compounds from A. cearensis have been described in several biological systems, ranging from antimicrobial to anti-inflammatory effects. Some of these activities are attributed to coumarins and phenolic compounds, the major compounds present in A. cearensis seed extracts. Multiple lines of research demonstrate these compounds reduce oxidative stress, inflammation and neuronal death induced by glutamate excitotoxicity, events central to most neuropathologies, including Alzheimer’s disease (AD) and Parkinson’s Disease (PD). This review focuses on the botanical aspects, folk medicine use, biological effects and pharmacological activities of A. cearensis compounds and their potential as novel non-toxic drugs for the treatment of neurodegenerative diseases.

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“…16 The hCA VII gene is thought to be involved in the mechanism of GABAergic excitement and seizure generation .17 Furthermore, its role controlling neuropathic pain has recently been hypothesized; however, the mechanism is unknown .18 Antibiotics are extensively used in human therapy to prevent and cure illnesses and promote growth .19 In addition, antibiotics play an important role in combating bacterial infections . 20 The term antibiotics is defined in medicine as compounds that can be natural or synthetic that neutralize microorganisms. Antibiotics must be targeted to the microorganism in order to have a beneficial effect.…”

Section: Introductionmentioning

confidence: 99%

“…Antibiotics are extensively used in human therapy to prevent and cure illnesses and promote growth. 19 In addition, antibiotics play an important role in combating bacterial infections. 20 The term antibiotics is defined in medicine as compounds that can be natural or synthetic that neutralize microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Recombinant human carbonic anhydrase VII: Purification, characterization, inhibition, and molecular docking studies

Duran

Beydemır

2022

Biotech and App Biochem

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Human carbonic anhydrase VII (hCA VII), a cytosolic enzyme, defends against oxidative stress by preventing reactive oxygen species from forming. In our study, first, hCA VII was cloned into Escherichia coli (One Shot Mach1‐T1R) strain by using cDNA of the human brain and successfully expressed. The integrity of the plasmid generated by colony PCR was checked, and after, for protein expression, the plasmid was transformed into E. coli BL21 (DE‐3) strain. hCA VII expression was observed after 6 h of isopropyl‐D‐1‐thiogalactopyranoside (IPTG) induction. The fusion protein containing hexahistidine (6xHis) was purified with 7.02 EU/mg of specific activity, had 48.07% of purification yield, and approximately 21‐folds using a ProbondTM nickel chelating resin affinity column. Then, both molecular mass determination and purity control of the purified recombinant enzyme was done by SDS‐PAGE (sodium dodecyl sulfate‐polyacrylamide gel electrophoresis). The mass of the SUMO‐hCA VII fusion protein was calculated as 46.77 kDa. As a result of Western blot analysis using anti‐His G‐HRP antibody, the fusion protein was detected as approximately 45 kDa. Furthermore, the characterization assays and in vitro inhibition studies were done for the recombinant enzyme. KI values of these agents were found between 0.29 μM and 157.6 mM. Finally, molecular docking investigations of these antibiotics were undertaken to understand further the binding interactions on the active site of this recombinant enzyme.

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Serine protease inhibition and modulatory-antibiotic activity of the proteic extract and fractions from Amburana cearensis

Cited by 9 publications

References 28 publications

UPLC-QTOF-MS/MS analysis and antibacterial activity of the Manilkara zapota (L.) P. Royen against Escherichia coli and other MDR bacteria

UPLC-QTOF-MS/MS analysis and antibacterial activity of the Manilkara zapota (L.) P. Royen against Escherichia coli and other MDR bacteria

Amburana cearensis: Pharmacological and Neuroprotective Effects of Its Compounds

Recombinant human carbonic anhydrase VII: Purification, characterization, inhibition, and molecular docking studies

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