Sericin from Bombyx mori cocoons. Part I: Extraction and physicochemical-biological characterization for biopharmaceutical applications

Rocha, Liliam K. H.; Favaro, Laura I. L.; Rios, Alessandra Cândida; Silva, Erica C.; Silva, Welida F.; Pasquoto-Stigliani, Tatiane; Guilger, Mariana; Lima, Renata de; Oliveira, José Martins de; Aranha, Norberto; Tubino, Matthieu; Vila, Marta Maria Duarte Carvalho; Balcão, Victor M.

doi:10.1016/j.procbio.2017.06.019

Cited by 65 publications

(44 citation statements)

References 44 publications

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“…In the present study, we extracted about 22.6% sericin from cocoons of silkworm by autoclaving the cocoons in the water at 121°C and high pressure for 30 min. These results were consistent with previous studies [45,51]. In another study, 28% of sericin was recovered from cocoons of B. mori [52].…”

Section: Discussionsupporting

confidence: 93%

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Synthesis of sericin‐conjugated silver nanoparticles and their potential antimicrobial activity

et al. 2020

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Nanoparticles (NPs) are being recognized as antibacterial agents due to their rapidly increasing multidrug resistance in bacterial pathogens. Hence, there is an unmet need to identify the natural antibacterial agent. The present study aimed to evaluate the antibacterial activity of sericin-conjugated silver NPs synthesized by using sericin as a reducing and capping agent. Synthesized NPs were characterized by scanning electron microscope, nanolaser particle size analyzer (BT-90), Fouriertransform infrared analysis, and energy-dispersive X-ray. The biogenic NPs significantly inhibited the growth of Escherichia coli (12-15 mm zone of inhibition), Staphylococcus aureus (14.6-15.4 mm zone of inhibition), and Klebsiella pneumoniae (12.5-18 mm zone of inhibition). The stability of naturally synthesized NPs was examined at various temperatures (i.e., 4°C, 37°C, and 55°C) and pH (i.e., 3, 7, and 11). Temperature variability did not significantly affect the efficacy of NPs. However, NPs performed better at higher pH levels. This study suggested that the sericinbased silver NPs are not only effective against bacteria, but they also maintain the stability at different ranges of temperature and pH. We concluded that the sericinconjugated silver NPs possess the remarkable antibacterial potential, which suggests their large-scale use as a cheap and stable antimicrobial agent in the future. K E Y W O R D Santibacterial activity, nanoparticles, sericin, silver

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Section: Discussionsupporting

confidence: 93%

“…In another study, 28% of sericin was recovered from cocoons of B. mori [52]. Other researchers such as Rocha et al [51] and He et al [48] also used similar methods for extraction of crude sericin from cocoons of B. mori . Other methods for extraction of sericin can also be used [3,5,20].…”

Section: Discussionmentioning

confidence: 99%

Synthesis of sericin‐conjugated silver nanoparticles and their potential antimicrobial activity

et al. 2020

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“…Percentage crystallinity for Muga silk was quantified to ~57.4% which closely matched with the values available in literatures suggesting that native unprocessed silks are typically 55‐60% crystalline . The degumming process resulted in removal of sericin, outer protective coating which is mainly amorphous . Devoid of sericin, the degummed silk showed enhanced crystallinity with increased peak intensities.…”

Section: Resultssupporting

confidence: 82%

Silk nano‐discs: A natural material for cancer therapy

et al. 2018

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The article demonstrates the crystalline silk nano‐discs (CSNs), with well‐controlled morphology, which upon magnetization, yields magnetic crystalline silk nano‐discs, making both prominent alternatives for replacing metal templates such as gold, silver, and so on in therapeutics and implants. The isolated β‐sheet‐rich discotic CSNs have ~50 nm diameter, high crystallinity (> 90%), and are insoluble but provide good dispersibility and stability in aqueous solutions. The melt blending‐cum‐electrospinning of functionalized CSN with poly(lactic acid) results in biocompatible nanofiber‐based scaffolds having in vitro cell cytocompatibility with improved cell adhesion and proliferation. The assessment of release behavior of curcumin, a naturally occurring anticancer drug, shows sustained release over 25 days exhibiting effective cytotoxicity against human cervical cancer cells. Further, combined effect of curcumin and hyperthermia reduced the cell growth by ~63%. Alignment of CSN‐derived magnetic nanoparticles due to effective fiber drawing process during electrospinning could improve cytocompatibility against BHK‐21 cells, and therefore efficacy for cancer therapy.

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“…Due to the high temperature applied, hydrogen bonds between hydroxyl groups become unstable allowing the water to interact with polar amino acids of the sericin (Silva et al, 2012). The molecular weight of the samples obtained is in the range of 27 to 200 kDa (Rocha et al, 2017). This method is quite simple, likewise a good quality and clean product is attained (Lamboni et al, 2015).…”

Section: Extraction With Steam Using Autoclavementioning

confidence: 99%

Extraction and antioxidant activity of sericin, a protein from silk

Miguel

Álvarez‐López

2020

Braz. J. Food Technol.

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Sericin is a globular protein that represents 20% to 30% of the silk fiber from Bombyx mori silkworm cocoon. This protein is usually removed from the raw fiber and discarded by silk producers, a process known as degumming. However, sericin possesses significant biological properties that allows its application in various fields. The antioxidant activity is one of its most relevant benefits. Several authors have reported its anti-tyrosinase activity, lipid peroxidation inhibition and free radical neutralization. The antioxidant potential of sericin protein varies according to the extraction method used. Even though a wide variety of extraction techniques have been studied, simple technics including water at high temperature have exhibited efficient results. Furthermore, this method does not interfere with the safety of sericin for subsequent applications in food.

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Sericin from Bombyx mori cocoons. Part I: Extraction and physicochemical-biological characterization for biopharmaceutical applications

Cited by 65 publications

References 44 publications

Synthesis of sericin‐conjugated silver nanoparticles and their potential antimicrobial activity

Synthesis of sericin‐conjugated silver nanoparticles and their potential antimicrobial activity

Silk nano‐discs: A natural material for cancer therapy

Extraction and antioxidant activity of sericin, a protein from silk

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