Serial plasma vasopressin concentration in healthy and hospitalised neonatal foals

Borchers, Angela; Magdesian, K. Gary; Schenck, Patricia A.; Kass, Philip H.

doi:10.1111/evj.12121

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…In contradiction to our results, Borchers et al . found no differences in osmolality between healthy, sick and septic foals . This difference could be due to the size of foal populations included in studies as well as criteria used to categorise foals (hypo‐ and normoperfused vs. healthy and septic).…”

Section: Discussionmentioning

confidence: 87%

Association of aldosterone and arginine vasopressin concentrations and clinical markers of hypoperfusion in neonatal foals

Dembek

Hurcombe

Stewart

et al. 2015

Equine Veterinary Journal

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Section: Discussionmentioning

confidence: 87%

Association of aldosterone and arginine vasopressin concentrations and clinical markers of hypoperfusion in neonatal foals

Dembek

Hurcombe

Stewart

et al. 2015

Equine Veterinary Journal

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“…To demonstrate AVP detection in the clinically relevant range (0–300 pM 38, 50, 51 ), we combined aptamer-based preconcentration of AVP with on-spot analyte enrichment, for which a dilute AVP solution was continuously infused into the device followed by washing, elution and repeated depositions on the MALDI plate. We first tested this approach for AVP detection in binding buffer, in which AVP concentrations were varied while the dDAVP concentration was kept constant (10 pM).…”

Section: Resultsmentioning

confidence: 99%

Integrated microfluidic aptasensor for mass spectrometric detection of vasopressin in human plasma ultrafiltrate

et al. 2016

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We present a microfluidic aptamer-based biosensor for detection of low-molecular-weight biomarkers in patient samples. Using a microfluidic device that integrates aptamer-based specific analyte extraction, isocratic elution, and detection by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, we demonstrate rapid, sensitive and label-free detection of arginine vasopressin (AVP) in human plasma ultrafiltrate. AVP molecules in complex matrices are specifically captured by an aptamer that is immobilized on microbeads via affinity binding in a microchamber. After the removal of unbound, contaminating molecules through washing, aptamer-AVP complexes are thermally disrupted via on-chip temperature control. Released AVP molecules are eluted with purified water and transferred to a separate microchamber, and deposited onto a single spot on a MALDI plate via repeated, piezoelectrically actuated ejection, which enriches AVP molecules over the spot area. This integrated on-chip sample processing enables the quantitative detection of low-abundance AVP by MALDI-TOF mass spectrometry in a rapid and label-free manner. Our experimental results show the detection of AVP in human plasma ultrafiltrate as low as physiologically relevant picomolar concentrations via aptamer-based selective preconcentration, demonstrating the potential of our approach as a rapid (~ 1hr), sensitive clinical AVP assay.

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“…The prognosis for survival to discharge of hospitalised septic foals has improved over time and ranges in recent studies from 44-71% Hurcombe et al 2008;Barsnick et al 2014;Borchers et al 2014;Toth et al 2014). Prognosis for athletic performance is considered to be good with surviving septic foals performing similar to age matched controls.…”

Section: Prognosismentioning

confidence: 99%

Pathophysiology, diagnosis and treatment of neonatal sepsis

Dunkel

Corley²

2014

Equine Veterinary Education

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Sepsis is a major cause of death in neonatal foals and, in recent years, significant progress in the understanding of the underlying pathophysiology has been made. To achieve a successful outcome, early diagnosis and treatment focusing on supporting vital functions and neutralising the effects of the causative organisms are essential. The pharmacokinetics of many drugs differ in neonatal foals and more information for appropriate dosing of antimicrobial and anti-inflammatory drugs for neonatal foals is now available to guide clinicians in choosing the best dosages. Prevention remains difficult and focuses on early recognition while prophylactic use of antimicrobials is discouraged.

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Serial plasma vasopressin concentration in healthy and hospitalised neonatal foals

Abstract: Plasma AVP concentrations are higher in septic foals on admission than in healthy and sick nonseptic foals. Higher early plasma AVP concentrations are associated with increased mortality.

Cited by 6 publications

References 37 publications

Association of aldosterone and arginine vasopressin concentrations and clinical markers of hypoperfusion in neonatal foals

Association of aldosterone and arginine vasopressin concentrations and clinical markers of hypoperfusion in neonatal foals

Integrated microfluidic aptasensor for mass spectrometric detection of vasopressin in human plasma ultrafiltrate

Pathophysiology, diagnosis and treatment of neonatal sepsis

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