Serial Passages of Parainfluenza Type 1 (Ha-2) Virus in the Continuous Cell Lines From Porcine, Rabbit and Green Monkey Kidneys Using the Trypsin-Added Systems

Itoh, Heihachi; Morimoto, Yuichi; Okawa, Shoichiro; Doi, Yutaka; Sanpe, Tsuneo; Nakajima, Minoru; Katoh, Toshitada; Sogawa, Yoshiharu

doi:10.7883/yoken1952.24.387

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1981

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“…3). Similar effects were reported for the growth of HVJ in L cells (Homma, 1971), parainfluenza types 1 and 4 in Vero cells (Morimoto et al, 1970;Itoh et al 1971) and NDV in MDBK cells (Nagai et al, 1976). It seems probable that Murayama virus grown in Vero or LLC-MK2 cells is not infectious in the respective cells and becomes infectious upon addition of trypsin by the same mechanism as that explained by Homma (1971).…”

Section: Discussionsupporting

confidence: 74%

Characteristics of Murayama Virus in Various Cell Cultures and Laboratory Animals

Nishikawa¹,

Yamamoto²,

Sugiyama³

1981

JJMSB

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Section: Discussionsupporting

confidence: 74%

Characteristics of Murayama Virus in Various Cell Cultures and Laboratory Animals

Nishikawa¹,

Yamamoto²,

Sugiyama³

1981

JJMSB

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Plaque formation by human-origin parainfluenza type 2 virus in established cell lines

Shimokata

Ito

Nishiyama

et al. 1981

Archives of Virology

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Two strains of parainfluenza type 2 virus formed well-defined plaques in cultures of Vero cells, an established line of African green monkey kidney cells. In the absence of trypsin, satisfactory plaques were formed by the Toshiba strain of virus. When trypsin was added in the overlay medium of Vero cell monolayers, another strain (62-M786) of virus produced plaques. The Toshiba strain was also able to make plaques in HeLa, BHK, and LLC-MK2 (an established line of monkey kidney) cells without trypsin, but not in mouse L cells. The sensitivity of plaque assay was about equal to that of the hemadsorption method.

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Serial Passages of Parainfluenza Type 1 (Ha-2) Virus in the Continuous Cell Lines From Porcine, Rabbit and Green Monkey Kidneys Using the Trypsin-Added Systems

Cited by 2 publications

References 4 publications

Characteristics of Murayama Virus in Various Cell Cultures and Laboratory Animals

Characteristics of Murayama Virus in Various Cell Cultures and Laboratory Animals

Plaque formation by human-origin parainfluenza type 2 virus in established cell lines

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