Serial passage in tissue culture of mixed foot-and-mouth disease virus serotypes

Woodbury, E. L.; Samuel, A. R.; Knowles, Nick J.

doi:10.1007/bf01309966

Cited by 8 publications

(5 citation statements)

References 11 publications

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“…Despite the stochastic generation of mutations, regular RNA viral competitions can follow a highly reproducible behaviour. This may also be at the basis of previous observations of viral fluctuations of foot-andmouth disease virus serotypes in tissue culture (Woodbury et al, 1995), and may also contribute to the long-term invariance of consensus sequences in spite of the continuous appearance of new mutant genomes (Domingo et al, 1978;Nichol et al, 1993;Steinhauer et al, 1989). A dynamical theory to explain biphasic fitness increases during large population passages of VSV (Novella et al, 1995a) has been recently presented (Tsimring et al, 1996).…”

Section: Discussionmentioning

confidence: 77%

Reproducible Nonlinear Population Dynamics and Critical Points During Replicative Competitions of RNA Virus Quasispecies

Quer

Huerta

Novella

et al. 1996

Journal of Molecular Biology

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Section: Discussionmentioning

confidence: 77%

Reproducible Nonlinear Population Dynamics and Critical Points During Replicative Competitions of RNA Virus Quasispecies

Quer

Huerta

Novella

et al. 1996

Journal of Molecular Biology

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“…The cyclical activity of FMD viruses has been previously observed at serotype levels (e.g. in Turkey, where outbreaks due to serotype O are often temporally interleaved with outbreaks sustained by viruses of serotype A and Asia 1 origin) 30 and experimentally (in cell culture) with type O/Asia 1 mixtures 31 , 32 . While the cyclical occurrence of FMDV serotypes might be driven by herd immunity against individual serotypes and introduction of viruses into naive animal populations, the evolutionary basis underpinning the cyclical occurrence of different genetic clades (and presence of several genomic variants) within a single sublineage is more difficult to explain, although the paucity of publically available sequence data from India from 2014 to date may have influenced these analyses due to sampling biases.…”

Section: Discussionmentioning

confidence: 87%

Reconstructing the evolutionary history of pandemic foot-and-mouth disease viruses: the impact of recombination within the emerging O/ME-SA/Ind-2001 lineage

Bachanek‐Bankowska

Nardo

Wadsworth

et al. 2018

Sci Rep

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Foot-and-mouth disease (FMD) is a highly contagious disease of livestock affecting animal production and trade throughout Asia and Africa. Understanding FMD virus (FMDV) global movements and evolution can help to reconstruct the disease spread between endemic regions and predict the risks of incursion into FMD-free countries. Global expansion of a single FMDV lineage is rare but can result in severe economic consequences. Using extensive sequence data we have reconstructed the global space-time transmission history of the O/ME-SA/Ind-2001 lineage (which normally circulates in the Indian sub-continent) providing evidence of at least 15 independent escapes during 2013–2017 that have led to outbreaks in North Africa, the Middle East, Southeast Asia, the Far East and the FMD-free islands of Mauritius. We demonstrated that sequence heterogeneity of this emerging FMDV lineage is accommodated within two co-evolving divergent sublineages and that recombination by exchange of capsid-coding sequences can impact upon the reconstructed evolutionary histories. Thus, we recommend that only sequences encoding the outer capsid proteins should be used for broad-scale phylogeographical reconstruction. These data emphasise the importance of the Indian subcontinent as a source of FMDV that can spread across large distances and illustrates the impact of FMDV genome recombination on FMDV molecular epidemiology.

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“…Competition between serotypes was significant, and under certain conditions, SAT-2 and SAT-3 challenge viruses were undetectable at later passages by qRT-PCR. Serial cell passage of mixed type O and Asia 1 infection was reported previously, with virus ratios up to passage 10 calculated by using an antigen ELISA (56). Virus extinction was not reported, and the two serotypes were shown to cycle equally through time.…”

Section: Discussionmentioning

confidence: 98%

Differential Persistence of Foot-and-Mouth Disease Virus in African Buffalo Is Related to Virus Virulence

Maree

Klerk-Lorist²,

Gubbins

et al. 2016

J Virol

103

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d ABSTRACTFoot-and-mouth disease (FMD) virus (FMDV) circulates as multiple serotypes and strains in many regions of endemicity. In particular, the three Southern African Territories (SAT) serotypes are maintained effectively in their wildlife reservoir, the African buffalo, and individuals may harbor multiple SAT serotypes for extended periods in the pharyngeal region. However, the exact site and mechanism for persistence remain unclear. FMD in buffaloes offers a unique opportunity to study FMDV persistence, as transmission from carrier ruminants has convincingly been demonstrated for only this species. Following coinfection of naive African buffaloes with isolates of three SAT serotypes from field buffaloes, palatine tonsil swabs were the sample of choice for recovering infectious FMDV up to 400 days postinfection (dpi). Postmortem examination identified infectious virus for up to 185 dpi and viral genomes for up to 400 dpi in lymphoid tissues of the head and neck, focused mainly in germinal centers. Interestingly, viral persistence in vivo was not homogenous, and the SAT-1 isolate persisted longer than the SAT-2 and SAT-3 isolates. Coinfection and passage of these SAT isolates in goat and buffalo cell lines demonstrated a direct correlation between persistence and cell-killing capacity. These data suggest that FMDV persistence occurs in the germinal centers of lymphoid tissue but that the duration of persistence is related to virus replication and cell-killing capacity. IMPORTANCEFoot-and-mouth disease virus (FMDV) causes a highly contagious acute vesicular disease in domestic livestock and wildlife species. African buffaloes (Syncerus caffer) are the primary carrier hosts of FMDV in African savannah ecosystems, where the disease is endemic. We have shown that the virus persists for up to 400 days in buffaloes and that there is competition between viruses during mixed infections. There was similar competition in cell culture: viruses that killed cells quickly persisted more efficiently in passaged cell cultures. These results may provide a mechanism for the dominance of particular viruses in an ecosystem.

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Serial passage in tissue culture of mixed foot-and-mouth disease virus serotypes

Cited by 8 publications

References 11 publications

Reproducible Nonlinear Population Dynamics and Critical Points During Replicative Competitions of RNA Virus Quasispecies

Reproducible Nonlinear Population Dynamics and Critical Points During Replicative Competitions of RNA Virus Quasispecies

Reconstructing the evolutionary history of pandemic foot-and-mouth disease viruses: the impact of recombination within the emerging O/ME-SA/Ind-2001 lineage

Differential Persistence of Foot-and-Mouth Disease Virus in African Buffalo Is Related to Virus Virulence

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