Serial monitoring of BCR–ABL by peripheral blood real‐time polymerase chain reaction predicts the marrow cytogenetic response to imatinib mesylate in chronic myeloid leukaemia

Wang, Lihui; Pillitteri, Lynne; Ferguson, Julia E.; Clark, Richard E.

doi:10.1046/j.1365-2141.2002.03705.x

Cited by 73 publications

(61 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pascot et al (13 ) may have found significant correlations for these relationships because they studied Ͼ400 individuals. Therefore, LLS analysis after chemical precipitation of apo B-containing lipoproteins gave results for HDL diameter in the same range as those obtained by established techniques for lipoprotein sizing (9,10,13 ). Furthermore, the differences between genders and the correlations reported here are in agreement with those described in the literature (13 ).…”

supporting

confidence: 81%

See 1 more Smart Citation

Quantitative Assessment of PML-RARa and BCR-ABL by Two Real-Time PCR Instruments: Multiinstitutional Laboratory Trial

et al. 2004

View full text Add to dashboard Cite

HDL particle aggregates after precipitation of apo Bcontaining lipoproteins.The data on HDL particle size obtained in this study (mean diameter, 8.8 nm) are in agreement with the values described in the literature for other techniques. In healthy normolipidemic individuals, HDL sizing by nuclear magnetic resonance spectroscopy yielded diameters of ϳ9.2 nm (10 ), whereas the values obtained from gradient gel electrophoresis ranged from 8.4 to 9.6 nm (9, 13 ). In this study, similar to other published results obtained by gradient gel electrophoresis (13 ), the HDL particle diameter (nm) was greater in premenopausal women than in men (9.1 vs 8.4 nm; Mann-Whitney test, P ϭ 0.013). Negative correlations between HDL particle size and total cholesterol and LDL-cholesterol were also reported by Pascot et al. (13 ), thus strengthening the link between HDL particle size and coronary artery disease. In the present study, the correlations between HDL particle size and HDL-cholesterol and triglyceride concentrations were not statistically significant, probably because of the small number of samples. Pascot et al. (13 ) may have found significant correlations for these relationships because they studied Ͼ400 individuals. Therefore, LLS analysis after chemical precipitation of apo B-containing lipoproteins gave results for HDL diameter in the same range as those obtained by established techniques for lipoprotein sizing (9,10,13 ). Furthermore, the differences between genders and the correlations reported here are in agreement with those described in the literature (13 ).Because LLS analysis after chemical precipitation is a practical and less time-consuming approach for HDL sizing, it could be used in large trials and in routine clinical laboratory analysis.

show abstract

supporting

confidence: 81%

“…Concentrations of urinary peptides serve not only as markers for kidney function but also as markers of other systemic physiologic processes. As examples, immunoassays for specific peptides provide measures of thrombosis and fibrinolysis (9,10 ) and endocrine function (11 ).…”

Section: Pml-raramentioning

confidence: 99%

Quantitative Assessment of PML-RARa and BCR-ABL by Two Real-Time PCR Instruments: Multiinstitutional Laboratory Trial

et al. 2004

View full text Add to dashboard Cite

show abstract

“…The BCR-ABL mRNA transcript level was measured by quantitative real time polymerase chain reaction (QRT-PCR) as previously described, 19 using ABL as the control gene, in line with recent recommendations. 20 For nested PCR studies, cDNA was amplified as described previously.…”

Section: Bcr-abl Mrna Quantitationmentioning

confidence: 99%

“…19 Complete cytogenetic remission (CCR) is defined as absence of Philadelphia chromosome positive (Ph þ ) metaphases and/or a BCR-ABL/ABL transcript ratio of less than 1%. We have previously demonstrated that a BCR-ABL/ABL transcript ratio of less than 1% correlates with CCR.…”

Section: Cytogenetic Studiesmentioning

confidence: 99%

Clinical evaluation of BCR-ABL peptide immunisation in chronic myeloid leukaemia: results of the EPIC study

et al. 2007

View full text Add to dashboard Cite

“…In contrast, DC preparations from all imatinib-treated cases not in CCR and all patients treated by other means (including one case in CCR treated with IFN only) were BCR-ABL positive. BCR-ABL mRNA expression was also quantified using a LightCycler (Roche Diagnostics Ltd, East Sussex, UK) as previously described 9 for chronic phase cases in whom DC BCR-ABL expression remained positive. Although the amount of BCR-ABL expression is variable between patients, in each case the DC BCR-ABL expression is consistently lower than the corresponding expression in WBC.…”

mentioning

confidence: 99%

Dendritic cells become BCR–ABL negative in chronic myeloid leukaemia patients successfully treated with imatinib

et al. 2004

View full text Add to dashboard Cite

of amplification facilitator. Taken together, these data show that only the addition of 0.04% BSA would allow a quantitative follow-up of the MRD. In the case of addition of 0.2% of BSA, there was a decrease in ABL CN (Figure 2a) and an increase in TEL-AML1 CN (Figure 2b) forbidding any accurate quantification. Actually, this effect was due to a variation of cycle threshold (C t ) value for the cDNA measurements with no modification of the plasmid standard curve (not shown).We studied 12 'poor-quality' samples of patients who have a clinical and/or biological disease and possess TEL-AML1 FG. In 70% of the cases with or without ABL amplification, we recovered TEL-AML1 expression by the addition of 0.04 and/or 0.2% BSA. However, only one TEL-AML1-positive sample had an increase in ABL CN higher than 1000 by the addition of BSA in RQ-PCR, allowing quantification of the TEL-AML1 expression 6 (Table 3).So, first, we have shown that the addition of BSA to TaqManbased RQ-PCR analysis improves amplification of 'poor-quality' samples. 6 Secondly, the addition of BSA is better than cDNA dilution or cDNA purification on column. Third, in certain cases, it allows improvement of relevant levels of FG like TEL-AML1.In a clinical laboratory, each patient sample set for molecular MRD analysis is very valuable, so any improvement of RNA quality even in some samples is suitable to apply in practice.Therefore, based on our data, this suggests that 0.04% BSA could be added systematically in ABL and TEL-AML1 RQ-PCR. This BSA concentration does not modify the RQ-PCR results of amplifiable samples and allows recovery to correct amplification of transcripts in some 'poor-quality' samples. Finally, a large prospective study is warranted to definitively assess the use of addition of 0.04% BSA in CG and FG RQ-PCR in a quantitative follow-up of the MRD.

show abstract

Serial monitoring of BCR–ABL by peripheral blood real‐time polymerase chain reaction predicts the marrow cytogenetic response to imatinib mesylate in chronic myeloid leukaemia

Cited by 73 publications

References 32 publications

Quantitative Assessment of PML-RARa and BCR-ABL by Two Real-Time PCR Instruments: Multiinstitutional Laboratory Trial

Quantitative Assessment of PML-RARa and BCR-ABL by Two Real-Time PCR Instruments: Multiinstitutional Laboratory Trial

Clinical evaluation of BCR-ABL peptide immunisation in chronic myeloid leukaemia: results of the EPIC study

Dendritic cells become BCR–ABL negative in chronic myeloid leukaemia patients successfully treated with imatinib

Contact Info

Product

Resources

About