Serial microPET measures of the metabolic reaction to a microdialysis probe implant

Schiffer, Wynne K.; Mirrione, Martine M.; Biegon, Anat; Alexoff, David; Patel, Vinal; Dewey, Stephen L.

doi:10.1016/j.jneumeth.2006.01.027

Cited by 170 publications

(155 citation statements)

References 51 publications

(56 reference statements)

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“…As a testament to the transitory nature of this mechanically induced wound healing response, electrode tracks could not be found in animals after several months when the electrode was inserted and quickly removed [6,13,35,47]. We have shown a similar effect with FDG, since glucose metabolism returned to normal levels within seven days if the microdialysis probe was inserted and immediately removed [39]. Taken together, it appears that injury alone produces a more transient metabolic response while the implant appears to induce a more enduring brain tissue response.…”

Section: Introductionmentioning

confidence: 81%

“…In experimental animals, surgical procedures such as cannulation, electrode placement and microdialysis probe implants are performed routinely in studies of brain organization and function. Studies in our laboratory and others have shown that following these routine surgical procedures, animals undergo dramatic short-term and long-term neurochemical and metabolic changes [5,34,39,41,46].…”

Section: Introductionmentioning

confidence: 95%

“…We have recently used serial small animal PET imaging to show a profound, persistent metabolic decline following microdialysis cannula implant that began as early as 2.0 hrs after the surgery, was at its lowest point 48 hrs after the surgery and persisted for the duration of the experiment, 25 days [39]. In a related series of studies, we have also used autoradiography to show that mice subjected to closed head injury undergo dynamic changes in glutamate NMDA receptor activation and availability [5].…”

Section: Introductionmentioning

confidence: 99%

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Metabolic and behavioral deficits following a routine surgical procedure in rats

et al. 2007

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To test the hypothesis that functional metabolic deficits observed following surgical brain injury are associated with changes in cognitive performance in rodents, we performed serial imaging studies in parallel with behavioral measures in control animals and in animals with surgical implants. Memory function was assessed using the novel object recognition (NOR) test, administered 3 days prior to and 3, 7, 14, and 56 days after surgery. At each time point, general locomotion was also measured. Metabolic imaging with 18 F-fluorodeoxyglucose ([ 18 F]FDG) occurred 28 and 58 days after surgery. Animals with surgical implants performed significantly worse on tests of object recognition, while general locomotion was unaffected by the implant. There was a significant decrease in glucose uptake after surgery in most of the hemisphere ipsilateral to the implant relative to the contralateral hemisphere. At both time points, the most significant metabolic deficits occurred in the primary motor cortex (-25%; p<0.001), sensory cortex (-15%, p<0.001) and frontal cortex (-12%; p<0.001). Ipsilateral areas further from the site of insertion became progressively worse, including the sensory cortex, dorsal striatum and thalamus. This data was supported by a voxel-based analysis of the PET data, which revealed again a unilateral decrease in [ 18 F]FDG uptake that extended throughout the ipsilateral cortex and persisted for the duration of the 58-day study. Probe implantation in the striatum results in a widespread and long lasting decline in cortical glucose metabolism together with a persistent, injury-related deficit in the performance of a cognitive (object recognition) task in rats.

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Section: Introductionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Metabolic and behavioral deficits following a routine surgical procedure in rats

et al. 2007

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“…Indeed, fixing the k′ 2 parameter used in analysis of raw images resulted in a negligible underestimation. To further confirm the argument that FA did not mitigate the signal integrity, we averaged the pixel-wise estimated BP ND values in each VOI [30], in raw and FAcorrected images, and compared them with the values estimated with SRTM2 at the VOI level in raw images. High correlations were found between pixel-wise estimated binding parameters and values estimated with VOI analysis.…”

Section: Discussionmentioning

confidence: 98%

“…At first, time frames of raw dynamic images between 30 and 80 min after tracer injection were averaged, in order to enhance visualization of the different structures and to manually co-register the anatomical data to an MRI and volume-of-interest (VOI) template from rat brain [30] implemented in PMOD software. Then, the co-registration parameters were applied to dynamic raw images and time-activity curves (TACs) were generated from the template VOIs.…”

Section: Image Analysismentioning

confidence: 99%

Quantification of GABAA receptors in the rat brain with [123I]Iomazenil SPECT from factor analysis-denoised images

Tsartsalis

Moulin-Sallanon

Dumas

et al. 2014

Nuclear Medicine and Biology

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Imaging of alcohol‐induced dopamine release in rats:Preliminary findings with [¹¹C]raclopride PET

Sullivan

Risacher

Normandin

et al. 2011

Synapse

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Microdialysis studies report that systemic alcohol increases extracellular dopamine in the rat striatum. The present study examined whether changes in striatal dopamine could be detected in rats using small animal positron emission tomography (PET). PET images were acquired in 44 alcohol-naïve male Wistar and alcohol-preferring (P) rats. Subjects received up to three [11C]raclopride scans (rest, alcohol, and saline). Animals were anesthetized with isoflurane and secured on a stereotactic-like holder during all scans. Blood samples were collected from the tail or lateral saphenous vein of 12 animals 10 minutes after tracer injection for determination of blood alcohol concentration (BAC). Time activity curves were extracted from the striatum and the cerebellum and binding potential (BPND) was calculated as a measure of D2 receptor availability. Wistars given 1.0g/kg alcohol (20%v/v) I.V. or 3.0g/kg alcohol (20%v/v) I.P showed significant alcohol-induced decreases in BPND. In P rats (given 1.5, 2.25, or 3.0 g/kg alcohol), no individual group showed a statistical effect of alcohol on BPND, but taken together, all P rats receiving I.P. alcohol had significantly lower BPND than rest or saline scans. Large decreases in BPND were primarily observed in rats with BAC above 200 mg%. Also, a significant difference was found between baseline BPND of Wistars who had undergone jugular catheterization surgery for I.V. alcohol administration and those who had not. These preliminary results suggest that alcohol-induced DA release in the rat striatum is detectable using small animal PET given sufficiently large cohorts and adequate blood alcohol levels.

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Serial microPET measures of the metabolic reaction to a microdialysis probe implant

Cited by 170 publications

References 51 publications

Metabolic and behavioral deficits following a routine surgical procedure in rats

Metabolic and behavioral deficits following a routine surgical procedure in rats

Quantification of GABAA receptors in the rat brain with [123I]Iomazenil SPECT from factor analysis-denoised images

Imaging of alcohol‐induced dopamine release in rats:Preliminary findings with [¹¹C]raclopride PET

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Serial microPET measures of the metabolic reaction to a microdialysis probe implant

Cited by 170 publications

References 51 publications

Metabolic and behavioral deficits following a routine surgical procedure in rats

Metabolic and behavioral deficits following a routine surgical procedure in rats

Quantification of GABAA receptors in the rat brain with [123I]Iomazenil SPECT from factor analysis-denoised images

Imaging of alcohol‐induced dopamine release in rats:Preliminary findings with [11C]raclopride PET

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Imaging of alcohol‐induced dopamine release in rats:Preliminary findings with [¹¹C]raclopride PET