Serial expression of the truncated fragments of the nucleocapsid protein of CCHFV and identification of the epitope region

Wei, P.C.; Luo, Yan-jun; Li, Tianxian; Wang, Hualin; Hú, Zhìhóng; Zhang, Fuchun; Zhāng, Yújiāng; Deng, Fei; Sun, Song

doi:10.1007/s12250-010-3067-7

Cited by 11 publications

(20 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, the highly conserved region 235 to 305 aa of CCHFV (YL04057 strain) N protein was identified as a highly antigenic region [31] .…”

Section: Discussionmentioning

confidence: 99%

“…FJ562093) of strain YL04057 CCHFV was kindly provided by Xinjiang CDC [31] . The complete NP ORF including the 5'-UTR and 3'-UTR was amplified using forward primer SF: 5'-TCTCAAAGAAACACGTGCCGC-3' and reverse primer SR: 5'-TCTCAAAGATATCGTT GCCGC-3' using Pyrobest DNA polymerase (TAKARA Bio Inc., Japan).…”

Section: Construction Of Donor Plasmids and Generation Of Recombinantmentioning

confidence: 99%

“…Western blot was also used to detect N proteins in different fractions after ultracentrifugation purification. Western blotting was performed according to Wei et al [31] with a slight modification. The primary antibody, anti-CCHFV N polyclonal rabbit serum [31] was used at 1:1000 dilution, and the secondary antibody, goat anti-rabbit immunoglobulin conjugated with alkaline phosphatase was used at 1:2000 dilution.…”

Section: Western Blot Analysesmentioning

confidence: 99%

“…Western blotting was performed according to Wei et al [31] with a slight modification. The primary antibody, anti-CCHFV N polyclonal rabbit serum [31] was used at 1:1000 dilution, and the secondary antibody, goat anti-rabbit immunoglobulin conjugated with alkaline phosphatase was used at 1:2000 dilution. Finally, the signals were detected by NBT/BCIP (nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate, SABC, China).…”

Section: Western Blot Analysesmentioning

confidence: 99%

“…These results indicate the nucleocapsid protein is a promising candidate for vaccine development [17,29] . The N protein of CCHFV is highly immunogenic [25,31] . In this paper, the N protein of CCHFV was expressed by the baculovirus expression system, which is a candidate for protein overexpression and is extensively used for vaccine development.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Production of CCHF virus-like particle by a baculovirus-insect cell expression system

et al. 2011

Self Cite

View full text Add to dashboard Cite

Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).

show abstract

“…Recently, the highly conserved region 235 to 305 aa of CCHFV (YL04057 strain) N protein was identified as a highly antigenic region [31] .…”

Section: Discussionmentioning

confidence: 99%

Section: Construction Of Donor Plasmids and Generation Of Recombinantmentioning

confidence: 99%

Section: Western Blot Analysesmentioning

confidence: 99%

Section: Western Blot Analysesmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Production of CCHF virus-like particle by a baculovirus-insect cell expression system

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology

Montoya-Ruiz

Rodas

2017

Methods in Molecular Biology

View full text Add to dashboard Cite

This article will outline surveillance approaches for viral hemorrhagic fevers. Specific methods for surveillance of clinical samples will be emphasized. Separate articles will describe methods for surveillance of rodent-borne viruses (roboviruses) and arthropod-borne viruses (arboviruses). Since the appearance of hantaviruses and arenaviruses in the Americas, more than 30 different species in each group have been established, and therefore they have become the most frequently emerging viruses. Flaviviruses such as yellow fever and dengue viruses, although easier to recognize, are also more widely spread and therefore considered a very important public health issue, particularly for under-developed countries. On the other hand, marburgviruses and ebolaviruses, previously thought to be restricted to the African continent, have recently been shown to be more global. For many of these agents virus isolation has been a challenging task: trapping the specific vectors (mosquitoes and ticks), and reservoirs (rodents and bats), or obtaining the samples from suspected clinical human cases demands special protective gear, uncommon devices (respirators), special facilities (BSL-3 and 4), and particular skills to recognize the slow and inapparent cytopathic effects in cell culture. Alternatively, serological and molecular approaches have been very helpful in discovering and describing newly emerging viruses in many areas where the previous resources are unavailable. Unfortunately, in many cases, detailed studies have been performed only after outbreaks occur, and then active surveillance is needed to prevent viral dissemination in human populations.

show abstract

Antigenicity of severe fever with thrombocytopenia syndrome virus nucleocapsid protein and its potential application in the virus serodiagnosis

et al. 2017

Self Cite

View full text Add to dashboard Cite

Serial expression of the truncated fragments of the nucleocapsid protein of CCHFV and identification of the epitope region

Cited by 11 publications

References 7 publications

Production of CCHF virus-like particle by a baculovirus-insect cell expression system

Production of CCHF virus-like particle by a baculovirus-insect cell expression system

Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology

Antigenicity of severe fever with thrombocytopenia syndrome virus nucleocapsid protein and its potential application in the virus serodiagnosis

Contact Info

Product

Resources

About