Serial analysis of gene expression (SAGE) in bovine trypanotolerance: preliminary results

Berthier, David; Quéré, Ronan; Thévenon, Sophie; Belemsaga, Désiré; Piquemal, David; Marti, Jacques; Maillard, Jean‐Charles

doi:10.1186/1297-9686-35-s1-s35

Cited by 15 publications

(1 citation statement)

References 27 publications

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“…There have been several previous genomic investigations of resistance to nematode infection including H. contortus in multiple sheep breeds and these results were compared with our Djallonke and Sahelian sheep results [36–40]. In contrast, there has been no previous genomic investigation of trypanosomiasis in any sheep breed; therefore, comparison was made with the reported trypanotolerance associated candidate genes in Ndama cattle [41–45].…”

Section: Methodsmentioning

confidence: 99%

Analysis of pooled genome sequences from Djallonke and Sahelian sheep of Ghana reveals co-localisation of regions of reduced heterozygosity with candidate genes for disease resistance and adaptation to a tropical environment

et al. 2019

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BackgroundThe Djallonke sheep is well adapted to harsh environmental conditions, and is relatively resistant to Haemonchosis and resilient to animal trypanosomiasis. The larger Sahelian sheep, which cohabit the same region, is less well adapted to these disease challenges. Haemonchosis and Trypanosomiasis collectively cost the worldwide animal industry billions of dollars in production losses annually.ResultsHere, we separately sequenced and then pooled according to breed the genomes from five unrelated individuals from each of the Djallonke and Sahelian sheep breeds (sourced from Ghana), at greater than 22-fold combined coverage for each breed. A total of approximately 404 million (97%) and 343 million (97%) sequence reads from the Djallonke and Sahelian breeds respectively, were successfully mapped to the sheep reference genome Oar v3.1. We identified approximately 11.1 million and 10.9 million single nucleotide polymorphisms (SNPs) in the Djallonke and Sahelian breeds, with approximately 15 and 16% respectively of these not previously reported in sheep. Multiple regions of reduced heterozygosity were also found; 70 co-localised within genomic regions harbouring genes that mediate disease resistance, immune response and adaptation in sheep or cattle. Thirty- three of the regions of reduced heterozygosity co-localised with previously reported genes for resistance to haemonchosis and trypanosomiasis.ConclusionsOur analyses suggest that these regions of reduced heterozygosity may be signatures of selection for these economically important diseases.

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Section: Methodsmentioning

confidence: 99%

Analysis of pooled genome sequences from Djallonke and Sahelian sheep of Ghana reveals co-localisation of regions of reduced heterozygosity with candidate genes for disease resistance and adaptation to a tropical environment

et al. 2019

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Inventory Analysis of West African Cattle Breeds

Belemsaga

Lombo

Thévenon

et al.

Applications of Gene-Based Technologies for Improving Animal Production and Health in Developing Countries

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A critical analysis of disease-associated DNA polymorphisms in the genes of cattle, goat, sheep, and pig

2008

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Genetic variations through their effects on gene expression and protein function underlie disease susceptibility in farm animal species. The variations are in the form of single nucleotide polymorphisms, deletions/insertions of nucleotides or whole genes, gene or whole chromosomal rearrangements, gene duplications, and copy number polymorphisms or variants. They exert varying degrees of effects on gene action, such as substitution of an amino acid for another, shift in reading frame and premature termination of translation, and complete deletion of entire exon(s) or gene(s) in diseased individuals. These factors influence gene function by affecting mRNA splicing pattern or by altering/eliminating protein function. Elucidating the genetic bases of diseases under the control of many genes is very challenging, and it is compounded by several factors, including host × pathogen × environment interactions. In this review, the genetic variations that underlie several diseases of livestock (under monogenic and polygenic control) are analyzed. Also, factors hampering research efforts toward identification of genetic influences on animal disease identification and control are highlighted. A better understanding of the factors analyzed could be better harnessed to effectively identify and control, genetically, livestock diseases. Finally, genetic control of animal diseases can reduce the costs associated with diseases, improve animal welfare, and provide healthy animal products to consumers, and should be given more attention.

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Serial analysis of gene expression (SAGE) in bovine trypanotolerance: preliminary results

Cited by 15 publications

References 27 publications

Analysis of pooled genome sequences from Djallonke and Sahelian sheep of Ghana reveals co-localisation of regions of reduced heterozygosity with candidate genes for disease resistance and adaptation to a tropical environment

Analysis of pooled genome sequences from Djallonke and Sahelian sheep of Ghana reveals co-localisation of regions of reduced heterozygosity with candidate genes for disease resistance and adaptation to a tropical environment

Inventory Analysis of West African Cattle Breeds

A critical analysis of disease-associated DNA polymorphisms in the genes of cattle, goat, sheep, and pig

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