Ser/Thr protein phosphatases in fungi: structure, regulation and function

Ariño, Joaquı́n; Velázquez, Diego; Casamayor, Antonio

doi:10.15698/mic2019.05.677

Cited by 55 publications

(57 citation statements)

References 359 publications

(445 reference statements)

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“…While PP1c enzymes are widely distributed among eukaryotes, Ppz phosphatases are found only in fungi [ 5 ]. Ppz phosphatases play an important role in the maintenance of monovalent cation homeostasis, although the specific mechanisms can be somewhat different between species [ 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

The N-Terminal Region of Yeast Protein Phosphatase Ppz1 Is a Determinant for Its Toxicity

Calafí

López-Malo

Albacar

et al. 2020

IJMS

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The Ppz enzymes are Ser/Thr protein phosphatases present only in fungi that are characterized by a highly conserved C-terminal catalytic region, related to PP1c phosphatases, and a more divergent N-terminal extension. In Saccharomyces cerevisiae, Ppz phosphatases are encoded by two paralog genes, PPZ1 and PPZ2. Ppz1 is the most toxic protein when overexpressed in budding yeast, halting cell proliferation, and this effect requires its phosphatase activity. We show here that, in spite of their conserved catalytic domain, Ppz2 was not toxic when tested under the same conditions as Ppz1, albeit Ppz2 levels were somewhat lower. Remarkably, a hybrid protein composed of the N-terminal extension of Ppz1 and the catalytic domain of Ppz2 was as toxic as Ppz1, even if its expression level was comparable to that of Ppz2. Similar amounts of yeast PP1c (Glc7) produced an intermediate effect on growth. Mutation of the Ppz1 myristoylable Gly2 to Ala avoided the localization of the phosphatase at the cell periphery but only slightly attenuated its toxicity. Therefore, the N-terminal extension of Ppz1 plays a key role in defining Ppz1 toxicity. This region is predicted to be intrinsically disordered and contains several putative folding-upon-binding regions which are absent in Ppz2 and might be relevant for toxicity.

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Section: Introductionmentioning

confidence: 99%

The N-Terminal Region of Yeast Protein Phosphatase Ppz1 Is a Determinant for Its Toxicity

Calafí

López-Malo

Albacar

et al. 2020

IJMS

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“…The putative proteins encode by ORF2, ORF3 and ORF4 of FgV1-ch showed five, one and six amino acid differences with proteins of FgV1-DK21, respectively. It is worth noting that among the six different amino acids of the putative protein encoded by ORF4 of FgV1-ch compared to FgV1-DK21, there are several serine and threonine changes, which may be phosphorylated easily and lead to functional changes [24,25]. As reported, SWI6, which is involved in the regulation of meiotic initiation, plays an important role in FgV1-DK21-induced phenotype alteration [26].…”

Section: Discussionmentioning

confidence: 87%

Molecular Characterization of a Novel Strain of Fusarium graminearum Virus 1 Infecting Fusarium graminearum

Zhang

Chen

Bhattacharjee

et al. 2020

Viruses

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Fungal viruses (mycoviruses) have attracted more attention for their possible hypovirulence (attenuation of fungal virulence) trait, which may be developed as a biocontrol agent of plant pathogenic fungi. However, most discovered mycoviruses are asymptomatic in their hosts. In most cases, mycovirus hypovirulent factors have not been explored clearly. In this study, we characterized a ssRNA mycovirus in Fusarium graminearum strain HB56-9. The complete nucleotide genome was obtained by combining random sequencing and rapid amplification of cDNA ends (RACE). The full genome was 6621-nucleotides long, excluding the poly(A) tail. The mycovirus was quite interesting because it shared 95.91% nucleotide identities with previously reported Fusarium graminearum virus 1 strain DK21 (FgV1-DK21), while the colony morphology of their fungal hosts on PDA plates were very different. The novel virus was named Fusarium graminearum virus 1 Chinese isolate (FgV1-ch). Like FgV1-DK21, FgV1-ch also contains four putative open reading frames (ORFs), including one long and three short ORFs. A phylogenetic analysis indicated that FgV1-ch is clustered into a proposed family Fusariviridae. FgV1-ch, unlike FgV1-DK21, had mild or no effects on host mycelial growth, spore production and virulence. The nucleotide differences between FgV1-ch and FgV1-DK21 will help to elucidate the hypovirulence determinants during mycovirus–host interaction.

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“…CDK also negatively regulates Cdc15 ( Jaspersen and Morgan, 2000 ; König et al, 2010 ). Finally, Clb5-Cdc28 might antagonize Cdc14 by direct phosphorylation ( Li et al, 2014 ), while the interplay between CDK thresholds and a constellation of phosphatases acting at the G 1 /S boundary is beginning to emerge ( Ariño et al, 2019 ; Martín et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase

Geymonat

Peng

Guo

et al. 2020

eLife

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Asymmetric astral microtubule organization drives the polarized orientation of the S. cerevisiae mitotic spindle and primes the invariant inheritance of the old spindle pole body (SPB, the yeast centrosome) by the bud. This model has anticipated analogous centrosome asymmetries featured in self-renewing stem cell divisions. We previously implicated Spc72, the cytoplasmic receptor for the gamma-tubulin nucleation complex, as the most upstream determinant linking SPB age, functional asymmetry and fate. Here we used structured illumination microscopy and biochemical analysis to explore the asymmetric landscape of nucleation sites inherently built into the spindle pathway and under the control of cyclin-dependent kinase (CDK). We show that CDK enforces Spc72 asymmetric docking by phosphorylating Nud1/centriolin. Furthermore, CDK-imposed order in the construction of the new SPB promotes the correct balance of nucleation sites between the nuclear and cytoplasmic faces of the SPB. Together these contributions by CDK inherently link correct SPB morphogenesis, age and fate.

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Ser/Thr protein phosphatases in fungi: structure, regulation and function

Cited by 55 publications

References 359 publications

The N-Terminal Region of Yeast Protein Phosphatase Ppz1 Is a Determinant for Its Toxicity

The N-Terminal Region of Yeast Protein Phosphatase Ppz1 Is a Determinant for Its Toxicity

Molecular Characterization of a Novel Strain of Fusarium graminearum Virus 1 Infecting Fusarium graminearum

Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase

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