Sequestering survivin to functionalized nanoparticles: a strategy to enhance apoptosis in cancer cells

Abstract

Survivin belongs to the family of inhibitor of apoptosis proteins (IAP) and is present in most cancers while being below detection limits in most terminally differentiated adult tissues, making it an attractive protein to target for diagnostic and, potentially, therapeutic roles. Sub-100 nm poly(propargyl acrylate) (PA) particles were surface modified through the copper-catalyzed azide/alkyne cycloaddition of an azide-terminated survivin ligand derivative (azTM) originally proposed by Abbott Labs and speculated to bind directly to survivin (protein) at its dimer interface. Using affinity pull-down studies, it was determined that the PA/azTM nanoparticles selectively bind survivin and the particles can enhance apoptotic cell death in glioblastomas and other survivin over-expressing cell lines such as A549 and MCF7 relative to cells incubated with the original Abbott-derived small molecule inhibitor.

Synthesis of Scintillating Ce3+-Doped Lu2Si2O7 Nanoparticles Using the Salt-Supported High Temperature (SSHT) Method: Solid State Chemistry at the Nanoscale

“…This can be useful in the functionalization of these particles for future biomedical applications such as optogenetics. 47,48 Figure 4 shows the powder X-ray diffraction (PXRD) patterns of the nanoparticles prepared using the SSHT method, and for comparison, Figure 5 shows the PXRD patterns of the nanoparticles heated at 1200 °C without the use of a salt support and of the sample prepared by solid state method at 1400 °C. As can be seen from Figure 4, the nanoparticles synthesized using the SSHT method at 1000 °C, the characteristic diffraction patterns confirm the presence of the Lu 2 Si 2 O 7 phase and indicate the presence of only minimal amounts of CeO 2 and unreacted silica.…”

“…After calcination the surface charge remain largely negative as expected and indicates that even after the SSHT treatment not all surface hydroxyl groups were removed. This can be useful in the functionalization of these particles for future biomedical applications such as optogenetics. , …”

Control of Vancomycin Activity through the Encapsulation and Controlled Release from a Propargyl Acrylate–Poloxamer Nanocomposite System

“…Described here is a drug delivery device composed of a biocompatible propargyl acrylate (PA) nanoparticle modified with a poloxamer block copolymer to achieve a temperature-triggered drug release. A PA core has shown to provide a versatile platform for medical applications. − Alkyne groups that decorate the surface of a PA nanoparticle serve as anchoring points that can be used to attach various active molecules through a copper­(I)-catalyzed azide/alkyne cycloaddition (CuAAC) click reaction. Click reactions offer the ability to covalently connect two molecules under mild conditions and with very high selectivity. , Click reactions are of particular interest when working with biologically active molecules due to the preserved biological activity of the molecules in the mild reaction conditions.…”