Sequential testing with different tissue transglutaminase antibodies, a new approach for diagnosis of celiac disease

Venugopal, Giriprasad; Mechenro, John; Makharia, Govind; Singh, Alka; Pugazhendhi, Srinivasan; Balamurugan, Ramadass; Ramakrishna, B. S.

doi:10.1007/s12664-017-0803-z

Cited by 5 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was believed that ELISA tests would be more easily accessible and fairly priced in the setting where the research was conducted and will be used in future clinical screenings. Our findings indicate that ELISA identified more positive results than RBA, which is in line with our previous studies demonstrating that ELISA had lower specificity but equivalent sensitivity to RBA ( 22 , 23 , 41 ). In addition, we found that HLA-DQ2 and/or DQ8 were presented in all CDA-positive children, with an exception of one child whose sequencing phase failed during the genetic analysis.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Incidence of celiac disease autoimmunity and associations with maternal tuberculosis and pediatric Helicobacter pylori infections in 4-year-old Ethiopian children followed up in an HLA genotyped birth cohort

Gudeta

Aronsson²,

Binagdie³

et al. 2022

Front. Pediatr.

View full text Add to dashboard Cite

BackgroundThe prevalence of celiac disease in the general population is mainly unknown in most of sub-Saharan African countries. The aim of this study was to determine the incidence of celiac disease autoimmunity (CDA) and its associations with latent Mycobacterium tuberculosis (LMTB) and Helicobacter pylori (HP) infections in Ethiopian children aged 4 years in an HLA genotyped cohort study.MethodsOf 1,389 recruited children between 2018 and 2022, 1,046 (75.3%) had been screened at least twice for celiac disease between the ages of 2 and 4 years using a tissue transglutaminase autoantibody (tTGA) ELISA kit. Tissue TGA-positive children were retested using radio-binding assays. CDA was defined as persistent-confirmed tTGA positivity in two consecutive samples. Associations of CDA with LMTB and HP were tested in a subpopulation of 752 children born to mothers who were previously tested for LMTB with IFN-γ and anti-HP antibodies in samples collected at a mean age of 49.3 ± 5.3 months.ResultsScreening detected 38 out of 1,046 (3.6%) IgA-tTGA-positive children. Ten (1.0%) were confirmed to be positive, with six (0.6%) children diagnosed with CDA. The incidence of CDA at 4 years of age was 1.2 per 1,000 person-years. LMTB was found in 4 of 6 (66.7%) mothers with CDA children compared with 340 of 734 (46.3%) mothers of children without CDA (p = 0.424), while HP was found in 3 of 6 (50.0%) CDA children compared with 315 of 746 (42.2%) children without CDA (p = 0.702).ConclusionThe incidence of CDA in Ethiopian children is lower than the pooled global incidence. Neither LMTB nor HP infections are associated with CD in Ethiopian children.

show abstract

Section: Discussionsupporting

confidence: 92%

“…Baseline screening for CDA started at 2 years of age by measuring IgA-neoepitopes against tTGA as previously described (AESKULISA tTg-A New generation, AESKU DIAGNOSTICS GmbH & Co. KG Mikroforum Ring 2 55234 Wendelsheim, Germany) ( 22 – 24 ). Antibody levels were expressed in U/ml.…”

Section: Methodsmentioning

confidence: 99%

Incidence of celiac disease autoimmunity and associations with maternal tuberculosis and pediatric Helicobacter pylori infections in 4-year-old Ethiopian children followed up in an HLA genotyped birth cohort

Gudeta

Aronsson²,

Binagdie³

et al. 2022

Front. Pediatr.

View full text Add to dashboard Cite

show abstract

“…Therefore, due to false positive results that are relatively common due to the high specificity of reverse immunofluorescence for the detection of EMA antibodies, the EMA test for patients with suspected celiac disease is often performed as a confirmatory test before biopsy in individuals who have a TTG-positive test. 17 The sensitivity and specificity of the TTG test in the diagnosis of celiac disease when the EMA test is also positive is about 100%. 18 Reverse immunofluorescence detection causes problems for EMA antibody observation due to different levels of observer skill, interference with anti-nuclear or anti-smooth muscle antibodies, as well as different standards from laboratory to laboratory in interpreting EMA testing.…”

Section: Discussionmentioning

confidence: 99%

Do Serological Tests Eliminate the Need for Endoscopic Biopsy for the Diagnosis of Symptomatic Patients with Celiac Disease? A Retrospective Study with Review of Literature

Anbardar,

Soleimani,

Torabi Dashtaki

et al. 2023

Middle East J Dig Dis

View full text Add to dashboard Cite

Background: Celiac disease is one of the most common genetic allergies worldwide. The prevalence of celiac disease in Iran is similar to or even higher than the global prevalence. Celiac disease is a chronic inflammatory disease that affects the small intestine. Affected patients are allergic to gluten protein that exists in some grains, such as wheat and barley. Methods: Serological endomysial IgA antibody (EMA-AB) and tissue transglutaminase IgA antibody (TTG-IgA) tests were performed on 114 patients aged the ages of 0–18 years with histopathological findings of celiac disease. The results of these tests were compared to the results of the histopathological study of the duodenal biopsy. Results: Based on the receiver operating characteristic (ROC) curve and a calculation of the TTG-IgA test’s sensitivity and specificity, the best diagnostic limit for the TTG-IgA test is 144, which has the best sensitivity and specificity. At this value (cut-off), the test’s sensitivity was 62%, and the specificity was 93.7%. For the endomysial test, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 80%, 93%, 90%, and 75%, respectively. Conclusion: The diagnostic accuracy of the endomysial test is better than that of the TTG-IgA test in general for diagnosing patients with celiac disease. In the TTG-IgA test, false-positive cases are high due to a cut-off of 20, reducing the test’s specificity. In these false-positive cases, the endomysial test helps in better diagnosis.

show abstract

“…In this issue of the Indian Journal of Gastroenterology, Venugopal et al [1] propose a two-tier auto-antibody testing approach, as a screening strategy for celiac disease. Making use of two sequential tests with opposing sensitivity and specificity is a new and interesting approach which could benefit patient care.…”

mentioning

confidence: 99%

“…This is followed by testing the resulting positive samples with a highly specific but less sensitive test [1]. This resulted in two transglutaminase IgA antibody (TGA) positive individuals (0.1%) within a group of 1917 healthy adults from three semi-urban areas in South India.…”

mentioning

confidence: 99%

A tandem approach of tTGA testing: A new approach for celiac disease screening

Bontkes

Mulder

2017

Indian J Gastroenterol

View full text Add to dashboard Cite

Sequential testing with different tissue transglutaminase antibodies, a new approach for diagnosis of celiac disease

Abstract: Sequential testing for anti-tTG using first a highly sensitive assay followed by a very specific assay is a new strategy for screening for CeD in clinical practice.

Cited by 5 publications

References 28 publications

Incidence of celiac disease autoimmunity and associations with maternal tuberculosis and pediatric Helicobacter pylori infections in 4-year-old Ethiopian children followed up in an HLA genotyped birth cohort

Incidence of celiac disease autoimmunity and associations with maternal tuberculosis and pediatric Helicobacter pylori infections in 4-year-old Ethiopian children followed up in an HLA genotyped birth cohort

Do Serological Tests Eliminate the Need for Endoscopic Biopsy for the Diagnosis of Symptomatic Patients with Celiac Disease? A Retrospective Study with Review of Literature

A tandem approach of tTGA testing: A new approach for celiac disease screening

Contact Info

Product

Resources

About