Sequential scCO2 drying and sterilisation of alginate-gelatine aerogels for biomedical applications

Bento, Cristiana S. A.; Alarico, Susana; Empadinhas, Nuno; Sousa, Hermínio C. de; Braga, Mara E.M.

doi:10.1016/j.supflu.2022.105570

Cited by 11 publications

(2 citation statements)

References 52 publications

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“…Thus, the reasons for the cellular decline on scCO 2 -sterilized hydrogels found in the present study may be complex and require further investigation. However, further studies are worthwhile, since low-temperature scCO 2 treatment, which is increasingly applied for temperature-sensitive natural polymers in current research, still has many advantages over standard procedures [ 22 , 31 , 50 , 51 ]. Nevertheless, based on the present results, this method of sterilization is not recommended for GEL- and HA-based hydrogels.…”

Section: Discussionmentioning

confidence: 99%

Effects of Gamma Irradiation and Supercritical Carbon Dioxide Sterilization on Methacrylated Gelatin/Hyaluronan Hydrogels

Heinemann

Büchner

Lee

et al. 2023

JFB

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Biopolymer hydrogels have become an important group of biomaterials in experimental and clinical use. However, unlike metallic or mineral materials, they are quite sensitive to sterilization. The aim of this study was to compare the effects of gamma irradiation and supercritical carbon dioxide (scCO2) treatment on the physicochemical properties of different hyaluronan (HA)- and/or gelatin (GEL)-based hydrogels and the cellular response of human bone marrow-derived mesenchymal stem cells (hBMSC). Hydrogels were photo-polymerized from methacrylated HA, methacrylated GEL, or a mixture of GEL/HA. The composition and sterilization methods altered the dissolution behavior of the biopolymeric hydrogels. There were no significant differences in methacrylated GEL release but increased methacrylated HA degradation of gamma-irradiated samples. Pore size/form remained unchanged, while gamma irradiation decreased the elastic modulus from about 29 kPa to 19 kPa compared to aseptic samples. HBMSC proliferated and increased alkaline phosphatase activity (ALP) particularly in aseptic and gamma-irradiated methacrylated GEL/HA hydrogels alike, while scCO2 treatment had a negative effect on both proliferation and osteogenic differentiation. Thus, gamma-irradiated methacrylated GEL/HA hydrogels are a promising base for multi-component bone substitute materials.

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Section: Discussionmentioning

confidence: 99%

Effects of Gamma Irradiation and Supercritical Carbon Dioxide Sterilization on Methacrylated Gelatin/Hyaluronan Hydrogels

Heinemann

Büchner

Lee

et al. 2023

JFB

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“…Subsequently, to improve the mechanical stability of biopolymers, a common strategy is to blend two or more polymers to construct double or multiple networks [39]. About 0.4% of gelatine was added to the SA solution [40] and stirred for 2 h at a temperature of 40 • C. Alginate structure consists of linear copolymers having blocks of D-mannuronic acid (M) and L-guluronic acid (G) residues [41,42]. The blocks are composed of consecutive G residues (GGGGGG), consecutive M residues (MMMMMM), and alternating M and G residues (GMGMGM).…”

Section: Experiments Section Materials and Methodsmentioning

confidence: 99%

Characterization of Composite Freeze-Dried Aerogels with Simulant Lunar Regolith for Space Applications

Borella,

Rozo,

Perfetti

et al. 2023

Materials

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Recently, the goal of space exploration has shifted from the incognito of the solar system to the Moon. Concepts like human permanence on the Moon and thermal protective structures made with ISRU (in situ resource utilization) of raw materials have started to be implemented. By limiting the need to launch supplies from the Earth, the paradigm of spaceflight is changed, privileging the vanguard of the utilisation of resources in situ. Still, the main challenges of surviving the radiation dose and the cryogenic temperatures of the lunar night remain. Recent studies have demonstrated how innovative composite materials can help reduce the temperature stress on exploration vehicles. This research presents the material properties of aerogel insulating materials combined with LHS (lunar highlands simulant) regolith obtained by freeze frying. Organic-based aerogels with different percentages of LHS have been analysed in terms of material, morphology, and thermal properties.

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Polysaccharide-based aerogels fabricated via supercritical fluid drying: a systematic review

Demina,

Minaev,

Akopova

2024

Polym. Bull.

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Sequential scCO2 drying and sterilisation of alginate-gelatine aerogels for biomedical applications

Cited by 11 publications

References 52 publications

Effects of Gamma Irradiation and Supercritical Carbon Dioxide Sterilization on Methacrylated Gelatin/Hyaluronan Hydrogels

Effects of Gamma Irradiation and Supercritical Carbon Dioxide Sterilization on Methacrylated Gelatin/Hyaluronan Hydrogels

Characterization of Composite Freeze-Dried Aerogels with Simulant Lunar Regolith for Space Applications

Polysaccharide-based aerogels fabricated via supercritical fluid drying: a systematic review

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