Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol

Vincent, Micky; Rence, Senawi Berry; Ennry, Esut; Norizawati, Muhammad Nor; Salwani, Awang Adeni Dayang

doi:10.17576/jsm-2015-4406-17

Cited by 10 publications

(22 citation statements)

References 14 publications

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“…The major components of SPW sample were consisted of starch (56.0 ± 2.0 wt%), cellulose (22.6 ± 1.0 wt%) and hemicellulose (7.80 ± 1.5 wt%), and lignin (5.50 ± 1.4 wt%) which were comparable with findings reported by Vincent et al [29]. The SPW was chosen as the prime feedstock material for the production of fermentable sugars since about 80 wt% of SPW consisted of starch and cellulose components.…”

Section: Sample Composition Analysissupporting

confidence: 90%

Conversion of Sago (Metroxylon sagu) Pith Waste to Fermentable Sugars via a Facile Depolymerization Process

Pang

Voon

Chin

2017

Appl Biochem Biotechnol

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The conversion of starchy sago (Metroxylon sagu) pith waste (SPW), a lignocellulosic biomass waste, to fermentable sugars under mild conditions had been successfully demonstrated. The optimum depolymerization of SPW was achieved at 2 wt% sample loading which was catalyzed by 100 mM of oxalic acid in the presence of 25 wt% NaCl solution at 110°C for 3 h. Up to 97% SPW sample was being converted into fermentable sugars with limited formation of by-products after two sequential depolymerization cycles. Both reaction temperature and concentration of oxalic acid were crucial parameters for the depolymerization of SPW which exhibited a high selectivity for the production of glucose over other reducing sugars.

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Section: Sample Composition Analysissupporting

confidence: 90%

Conversion of Sago (Metroxylon sagu) Pith Waste to Fermentable Sugars via a Facile Depolymerization Process

Pang

Voon

Chin

2017

Appl Biochem Biotechnol

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“…Pertumbuhan jamur di alam hidup pada substrat secara saprofit yang mengandung zat nutrisi selulosa dimana banyak terdapat pada kayu lapuk seperti pada media serbuk gergaji (Imelda dkk, 2015). Menurut Suharnowo dkk, (2012) (Vincent et al, 2015).…”

Section: Pendahuluanunclassified

PENGAMATAN PERTUMBUH MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq). P.kummer) PADA MEDIA DASAR SERBUK GERGAJI DENGAN SUPLEMENTASI AMPAS SAGU

Hidayat

Umrah

Ananda

2019

Bioceb

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The study of observation of growth media formulation of white oyster mushrooms’s mycelium (Pleurotus ostreatus (Jacq.) P.Kummer) on the base with sago pulp supplementation medium was conducted from March to May 2018 at Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. This study were aimed to determine the appropriate formulation and dosage as supplementation of sago pulp with basic media for the growth of the white oyster mushroom mycelium. This study was conducted by Randomized Complete Random Design (RAL), consisted of seven treatments and four replications with basic media and supplementary M0 (100%: without supplements), M1 (95% : 5%), M2 (90% : 10%), M3 (85% : 15%), M4 (80% : 20%), M5 (75% : 25%), M6 (70% : 30%). The results showed that the best growth of mycelium on treatment M0 and M1, mycelium filled the media for 26 days with the average growth of mycelium M0 and M1 were 0,56 cm/ day. The best formulation and dosage of sago suplementation on the base medium were found in M1 (95% base medium: 5% sago pulp).

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“…In this study, only the starchy material was hydrolysed by amyloglucosidase and the lignocellulose remained intact. Thus, further hydrolysis of cellulosic residue using cellulase enzyme can be applied to achieve a complete conversion of SPR into fermentable sugars by breaking the 1,4-β-D-glycosidic linkage (Vincent et al 2015). Vincent et al (2015) studies a process in which the starchy material was first liquefied and saccharides, followed by the hydrolysis of cellulosic residue using cellulase via sequential saccharification and simultaneous fermentation (SSSF).…”

Section: Microscopic Analysismentioning

confidence: 99%

“…Thus, further hydrolysis of cellulosic residue using cellulase enzyme can be applied to achieve a complete conversion of SPR into fermentable sugars by breaking the 1,4-β-D-glycosidic linkage (Vincent et al 2015). Vincent et al (2015) studies a process in which the starchy material was first liquefied and saccharides, followed by the hydrolysis of cellulosic residue using cellulase via sequential saccharification and simultaneous fermentation (SSSF). However, as silica may physically impact enzymatic attack, in this case, an effective removal of the silica during pretreatment is necessary to enhance the digestibility of SPR fibre.…”

Section: Microscopic Analysismentioning

confidence: 99%

“…SPR on the other hand, contained a starchy lignocellulosic material which can easily be converted into fermentable sugar. Vincent et al (2015) managed to obtain 8 g of sugar from 10 g SPR using enzymatic hydrolysis. Although the sugar in the previous studies reported by Awg-Adeni et al (2013) and Vincent et al (2015) were slightly higher compared to our study (7.34 g sugar from 10 g SPR), the enzymatic approach might not be economically attractive to the production of a low-cost fuel such as ethanol.…”

Section: Overall Mass Balancementioning

confidence: 99%

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Hydrolysis of Residual Starch from Sago Pith Residue and its Fermentation to Bioethanol

Bukhari¹,

Loh²,

Nasrin³

et al. 2017

JSM

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Utilisation of sago pith residue (SPR) for fermentable sugar production using both acid and enzymatic hydrolysis was studied. In acid hydrolysis, the effect of solid and acid concentrations, temperature and reaction time was optimised. The effect of enzyme dosage was studied on enzymatic hydrolysis of SPR. Higher yield and conversion of 0.73 g g-1 (96% conversion) was achieved by treating 6% (w v-1) of SPR with 1% (v v-1) H 2 SO 4 at 125°C for 90 min as compared to 0.61 g g-1 (79% conversion) using 40 U g-1 biomass of Aspergillus niger amyloglucosidase incubated at 60°C and pH4 for 48 h. The fermentation of acid hydrolysate of SPR demonstrated that high ethanol yield of 98% can be achieved without supplementation of nitrogen and nutrients. The complete process showed that 470 L of bioethanol could be produced from 1 tonne of SPR. This figure makes SPR an ideal raw material for bio-conversion into bioethanol or other value-added products.

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Sequential Saccharification and Simultaneous Fermentation (SSSF) of Sago Hampas for the Production of Bioethanol

Abstract: Bioethanol is a very environmentally friendly liquid biofuel that is not

Cited by 10 publications

References 14 publications

Conversion of Sago (Metroxylon sagu) Pith Waste to Fermentable Sugars via a Facile Depolymerization Process

Conversion of Sago (Metroxylon sagu) Pith Waste to Fermentable Sugars via a Facile Depolymerization Process

PENGAMATAN PERTUMBUH MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq). P.kummer) PADA MEDIA DASAR SERBUK GERGAJI DENGAN SUPLEMENTASI AMPAS SAGU

Hydrolysis of Residual Starch from Sago Pith Residue and its Fermentation to Bioethanol

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