Sequential Roles of Brg, the ATPase Subunit of BAF Chromatin Remodeling Complexes, in Thymocyte Development

Tian, Hui; Wan, Mimi; Lee, Peggy P.; Akashi, Koichi; Metzger, Daniel; Chambon, Pierre; Wilson, Christopher; Crabtree, Gerald R.

doi:10.1016/s1074-7613(03)00199-7

Cited by 149 publications

(150 citation statements)

References 52 publications

Supporting

Mentioning

144

Contrasting

Order By: Relevance

“…In CD8 cells, the repression, once established, is selfperpetuating and maintained in an epigenetic manner independently of the silencer, suggesting that CD4 repression in CD8, but not DN, cells is mediated by heterochromatin (5). Multiple trans-acting factors have been implicated in CD4 regulation, including the chromatin remodeling factors Brg, BAF57, and Mi-2b; histone acetyl transferase p300; E-box-binding protein HEB/E2A; Ets-domain protein Elf-1; c-Myb; Myc-associated zinc finger protein (MAZ); Ikaros; T cell factor-1 (TCF-1); and the Runt-domain proteins Runx1 and Runx3 essential for CD4 repression in DN and CD8 cells, respectively (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). However, little is known about the molecular mechanisms whereby these cis-acting elements and transcription factors control CD4 transcription.…”

mentioning

confidence: 99%

Nucleoprotein structure of the CD4 locus: Implications for the mechanisms underlying CD4 regulation during T cell development

Wan

Zhang

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

A multiplex PCR assay for semiquantitative measurement of the CD4 enhancer (E), silencer (S), promoter (P), and the GADPH gene (asterisk). The agarose gel is stained with SYBR green, which gives less background staining and a wider dynamic range than ethidium bromide.(B and C) ChIP assays showing histone modifications (B) and transcription factor binding (C) at the CD4 locus. Two million cell equivalents of chromatin were used for each immunoprecipitation, and 10% of precipitated DNA was analyzed by PCR. At least two independent sources of cells were used for chromatin preparation, and PCR was repeated at least three times for each precipitated DNA sample. Input, sonicated chromatin equivalent to 2,000 cells and, thus, representing 0.1% of the starting material; DN, double-negative thymocytes from Rag2 Ϫ/Ϫ mice. (D) Quantitative analysis of ChIP samples. DNA from two experiments was quantified and the values averaged. Fold enrichment was then determined as described in Materials and Methods. R;S, DN3 cells isolated from Rag2 Ϫ/Ϫ ; silencer Ϫ/Ϫ mice as described for Fig. 3.

show abstract

mentioning

confidence: 99%

Nucleoprotein structure of the CD4 locus: Implications for the mechanisms underlying CD4 regulation during T cell development

Wan

Zhang

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Specific inactivation of SWI/SNF complexes in T cells demonstrates that such complexes are essential for thymocyte development. SWI/SNF further contributes to CD4/CD8 T-cell lineage divergence by repressing CD4 receptor expression while activating the expression of CD8 (Chi et al, 2002(Chi et al, , 2003Gebuhr et al, 2003). Variable-diversity-joining (V(D)J) recombination has also been shown to be mediated by SWI/SNF.…”

Section: Brm and Brg1 Control The Expression Of Genes That Are Involvmentioning

confidence: 99%

The SWI/SNF complex and cancer

2009

View full text Add to dashboard Cite

The mammalian SWI/SNF complexes mediate ATPdependent chromatin remodeling processes that are critical for differentiation and proliferation. Not surprisingly, loss of SWI/SNF function has been associated with malignant transformation, and a substantial body of evidence indicates that several components of the SWI/SNF complexes function as tumor suppressors. This review summarizes the evidence that underlies this conclusion, with particular emphasis upon the two catalytic subunits of the SWI/SNF complexes, BRM, the mammalian ortholog of SWI2/SNF2 in yeast and brahma in Drosophila, and Brahma-related gene-1 (BRG1).

show abstract

“…For this reason, in vivo studies of SWI/SNF functions in mammalian cells have relied more strongly on dominant-negative approaches and on the SW13 cell line, which has adapted to growth in the absence of SWI/SNF complexes, than on mice or cells with disrupted SWI/ SNF alleles (de La Serna et al 2000;Liu et al 2001;Chi et al 2003;Chi 2004). As an alternative strategy for performing loss-of-function studies, we disrupted expression of remodeling complexes by retroviral delivery of small interfering RNAs (siRNAs) and focused on gene activation in response to LPS, an acute extracellular stimulus.…”

mentioning

confidence: 99%

Selective and antagonistic functions of SWI/SNF and Mi-2β nucleosome remodeling complexes during an inflammatory response

Ramirez-Carrozzi¹,

Nazarian²,

Li³

et al. 2006

Genes Dev.

390

418

View full text Add to dashboard Cite

Studies of mammalian genes activated in response to an acute stimulus have suggested diverse mechanisms through which chromatin structure and nucleosome remodeling events contribute to inducible gene transcription. However, because of this diversity, the logical organization of the genome with respect to nucleosome remodeling and gene induction has remained obscure. Numerous proinflammatory genes are rapidly induced in macrophages in response to microbial infection. Here, we show that in lipopolysaccharide-stimulated macrophages, the catalytic BRG1/BRM subunits of the SWI/SNF class of ATP-dependent nucleosome remodeling complexes are consistently required for the activation of secondary response genes and primary response genes induced with delayed kinetics, but not for rapidly induced primary response genes. Surprisingly, a Mi-2␤ complex was selectively recruited along with the SWI/SNF complexes to the control regions of secondary response and delayed primary response genes, with the Mi-2␤ complex acting antagonistically to limit the induction of these gene classes. SWI/SNF and Mi-2␤ complexes influenced cell size in a similarly antagonistic manner. These results provide insight into the differential contributions of nucleosome remodeling complexes to the rapid induction of defined classes of mammalian genes and reveal a robust anti-inflammatory function of Mi-2␤.[Keywords: Inflammation; cytokines; chromatin; SWI/SNF; NuRD] Supplemental material is available at http://www.genesdev.org.

show abstract

Sequential Roles of Brg, the ATPase Subunit of BAF Chromatin Remodeling Complexes, in Thymocyte Development

Cited by 149 publications

References 52 publications

Nucleoprotein structure of the CD4 locus: Implications for the mechanisms underlying CD4 regulation during T cell development

Nucleoprotein structure of the CD4 locus: Implications for the mechanisms underlying CD4 regulation during T cell development

The SWI/SNF complex and cancer

Selective and antagonistic functions of SWI/SNF and Mi-2β nucleosome remodeling complexes during an inflammatory response

Contact Info

Product

Resources

About