Sequential resolution of (S) and (R)-6-fluoro-chroman-2-carboxylic acid by two esterases in turn

Abstract

Optically pure 6-fluoro-chroman-2-carboxylic acids (FCCAs), including (S) and (R)- configuration, are pivotal chiral building blocks in the pharmaceutical industry. To date, the production of (S) and (R)-FCCA mainly depends on...

Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

“…388 Various biocatalytic methods for the synthesis of carboxylic acids have been developed, complementing nonenzymatic approaches. 389 These methods include the hydrolysis of nitriles by nitrilases, 390−392 the conversion of aldehydes by hydroxynitrile lyases with subsequent hydrolysis, 393 the hydrolysis of esters by esterases, 394 and the oxidation of alcohols or aldehydes by dehydrogenases and oxidases. 395 Glieder et al reported a hydroxynitrile lyase mutant from Prunus amygdalus for the enantioselective synthesis of α-hydroxyacids by the addition of HCN to aldehydes and the subsequent hydrolysis of the intermediately formed cyanohydrins.…”

“…Various biocatalytic methods for the synthesis of carboxylic acids have been developed, complementing nonenzymatic approaches . These methods include the hydrolysis of nitriles by nitrilases, − the conversion of aldehydes by hydroxynitrile lyases with subsequent hydrolysis, the hydrolysis of esters by esterases, and the oxidation of alcohols or aldehydes by dehydrogenases and oxidases …”